Experiment: m.b. Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1709 and Dshi_1710 are separated by 230 nucleotides Dshi_1710 and Dshi_1711 overlap by 8 nucleotides Dshi_1711 and Dshi_1712 overlap by 1 nucleotides Dshi_1712 and Dshi_1713 are separated by 98 nucleotides
Dshi_1709: Dshi_1709 - MscS Mechanosensitive ion channel (RefSeq), at 1,776,333 to 1,777,817
_1709
Dshi_1710: Dshi_1710 - glutaredoxin-like protein (RefSeq), at 1,778,048 to 1,778,410
_1710
Dshi_1711: Dshi_1711 - hypothetical protein (RefSeq), at 1,778,403 to 1,778,669
_1711
Dshi_1712: Dshi_1712 - BolA family protein (RefSeq), at 1,778,669 to 1,778,902
_1712
Dshi_1713: Dshi_1713 - phosphoribosylformylglycinamidine synthase II (RefSeq), at 1,779,001 to 1,781,172
_1713
Position (kb)
1778
1779 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1777.443 kb on - strand, within Dshi_1709 at 1777.572 kb on - strand, within Dshi_1709 at 1777.647 kb on + strand, within Dshi_1709 at 1777.806 kb on - strand at 1777.893 kb on + strand at 1778.098 kb on - strand, within Dshi_1710 at 1778.420 kb on - strand at 1778.610 kb on + strand, within Dshi_1711 at 1778.662 kb on - strand at 1778.964 kb on + strand at 1779.024 kb on + strand at 1779.405 kb on - strand, within Dshi_1713 at 1779.525 kb on + strand, within Dshi_1713 at 1779.576 kb on + strand, within Dshi_1713
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzalkonium Chloride 0.0001 mg/ml remove 1,777,443 - Dshi_1709 0.75 +0.8 1,777,572 - Dshi_1709 0.83 +0.2 1,777,647 + Dshi_1709 0.88 +1.6 1,777,806 - -0.2 1,777,893 + +0.1 1,778,098 - Dshi_1710 0.14 +0.8 1,778,420 - +1.2 1,778,610 + Dshi_1711 0.78 -4.0 1,778,662 - -0.5 1,778,964 + +0.8 1,779,024 + +3.4 1,779,405 - Dshi_1713 0.19 -0.2 1,779,525 + Dshi_1713 0.24 +1.8 1,779,576 + Dshi_1713 0.26 -1.2
Or see this region's nucleotide sequence