Experiment: m.b. Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0218 and Dshi_0219 are separated by 3 nucleotides Dshi_0219 and Dshi_0220 are separated by 84 nucleotides Dshi_0220 and Dshi_0221 are separated by 93 nucleotides
Dshi_0218: Dshi_0218 - ATP--cobalamin adenosyltransferase (RefSeq), at 216,003 to 216,575
_0218
Dshi_0219: Dshi_0219 - hypothetical protein (RefSeq), at 216,579 to 216,794
_0219
Dshi_0220: Dshi_0220 - short-chain dehydrogenase/reductase SDR (RefSeq), at 216,879 to 217,712
_0220
Dshi_0221: Dshi_0221 - DNA topoisomerase IV, A subunit (RefSeq), at 217,806 to 220,139
_0221
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 215.952 kb on - strand at 216.009 kb on - strand at 216.079 kb on - strand, within Dshi_0218 at 216.156 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.364 kb on - strand, within Dshi_0218 at 216.564 kb on + strand at 216.564 kb on + strand at 216.579 kb on - strand at 216.579 kb on - strand at 216.782 kb on - strand at 216.830 kb on + strand at 216.833 kb on - strand at 216.834 kb on + strand at 216.838 kb on - strand at 216.927 kb on - strand at 216.985 kb on - strand, within Dshi_0220 at 216.990 kb on - strand, within Dshi_0220 at 217.008 kb on - strand, within Dshi_0220 at 217.018 kb on + strand, within Dshi_0220 at 217.018 kb on + strand, within Dshi_0220 at 217.037 kb on + strand, within Dshi_0220 at 217.045 kb on - strand, within Dshi_0220 at 217.178 kb on + strand, within Dshi_0220 at 217.240 kb on - strand, within Dshi_0220 at 217.290 kb on - strand, within Dshi_0220 at 217.300 kb on - strand, within Dshi_0220 at 217.302 kb on + strand, within Dshi_0220 at 217.310 kb on - strand, within Dshi_0220 at 217.310 kb on - strand, within Dshi_0220 at 217.423 kb on + strand, within Dshi_0220 at 217.468 kb on + strand, within Dshi_0220 at 217.480 kb on + strand, within Dshi_0220 at 217.525 kb on + strand, within Dshi_0220 at 217.529 kb on + strand, within Dshi_0220 at 217.587 kb on - strand, within Dshi_0220 at 217.626 kb on - strand, within Dshi_0220 at 217.677 kb on + strand at 217.687 kb on + strand at 217.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzalkonium Chloride 0.0001 mg/ml remove 215,952 - -0.1 216,009 - +0.6 216,079 - Dshi_0218 0.13 +2.1 216,156 - Dshi_0218 0.27 +1.3 216,359 - Dshi_0218 0.62 -1.8 216,359 - Dshi_0218 0.62 +0.6 216,364 - Dshi_0218 0.63 +1.9 216,564 + -1.5 216,564 + -1.8 216,579 - -0.3 216,579 - +1.2 216,782 - +0.0 216,830 + -1.0 216,833 - +3.0 216,834 + +0.8 216,838 - -0.5 216,927 - -1.0 216,985 - Dshi_0220 0.13 -1.3 216,990 - Dshi_0220 0.13 +0.1 217,008 - Dshi_0220 0.15 -0.3 217,018 + Dshi_0220 0.17 +0.4 217,018 + Dshi_0220 0.17 +1.0 217,037 + Dshi_0220 0.19 -0.4 217,045 - Dshi_0220 0.20 -0.3 217,178 + Dshi_0220 0.36 -0.6 217,240 - Dshi_0220 0.43 +3.0 217,290 - Dshi_0220 0.49 +1.0 217,300 - Dshi_0220 0.50 -1.2 217,302 + Dshi_0220 0.51 -3.3 217,310 - Dshi_0220 0.52 -0.3 217,310 - Dshi_0220 0.52 +0.5 217,423 + Dshi_0220 0.65 -1.8 217,468 + Dshi_0220 0.71 +1.1 217,480 + Dshi_0220 0.72 -0.1 217,525 + Dshi_0220 0.77 -2.0 217,529 + Dshi_0220 0.78 +0.0 217,587 - Dshi_0220 0.85 +0.3 217,626 - Dshi_0220 0.90 +1.0 217,677 + -0.1 217,687 + -2.7 217,777 - +0.8
Or see this region's nucleotide sequence