Experiment: m.b. Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0178 and Dshi_0179 are separated by 73 nucleotides Dshi_0179 and Dshi_0180 are separated by 72 nucleotides Dshi_0180 and Dshi_0181 overlap by 4 nucleotides
Dshi_0178: Dshi_0178 - aminotransferase class I and II (RefSeq), at 181,801 to 182,973
_0178
Dshi_0179: Dshi_0179 - peptide deformylase (RefSeq), at 183,047 to 183,610
_0179
Dshi_0180: Dshi_0180 - peptide deformylase (RefSeq), at 183,683 to 184,189
_0180
Dshi_0181: Dshi_0181 - methionyl-tRNA formyltransferase (RefSeq), at 184,186 to 185,085
_0181
Position (kb) 183
184
185 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 182.730 kb on - strand, within Dshi_0178 at 182.770 kb on + strand, within Dshi_0178 at 182.874 kb on + strand at 182.939 kb on + strand at 182.947 kb on - strand at 182.968 kb on + strand at 182.976 kb on - strand at 182.988 kb on - strand at 183.760 kb on + strand, within Dshi_0180 at 183.827 kb on + strand, within Dshi_0180 at 183.959 kb on - strand, within Dshi_0180 at 184.057 kb on + strand, within Dshi_0180 at 184.077 kb on - strand, within Dshi_0180 at 184.090 kb on + strand, within Dshi_0180 at 184.168 kb on + strand at 184.193 kb on - strand at 185.057 kb on + strand at 185.109 kb on + strand at 185.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzalkonium Chloride 0.0001 mg/ml remove 182,730 - Dshi_0178 0.79 -3.3 182,770 + Dshi_0178 0.83 -0.2 182,874 + +2.3 182,939 + -0.3 182,947 - +1.0 182,968 + +1.4 182,976 - -2.9 182,988 - +0.3 183,760 + Dshi_0180 0.15 -1.0 183,827 + Dshi_0180 0.28 -2.0 183,959 - Dshi_0180 0.54 +2.3 184,057 + Dshi_0180 0.74 -0.6 184,077 - Dshi_0180 0.78 +3.6 184,090 + Dshi_0180 0.80 -2.6 184,168 + +4.0 184,193 - -3.3 185,057 + -0.6 185,109 + +1.0 185,189 - +1.0
Or see this region's nucleotide sequence