Experiment: m.b. Benzalkonium Chloride 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0025 and Dshi_0026 are separated by 73 nucleotides Dshi_0026 and Dshi_0027 are separated by 123 nucleotides Dshi_0027 and Dshi_0028 are separated by 7 nucleotides
Dshi_0025: Dshi_0025 - anti-ECFsigma factor, ChrR (RefSeq), at 29,143 to 29,814
_0025
Dshi_0026: Dshi_0026 - NAD-dependent epimerase/dehydratase (RefSeq), at 29,888 to 30,529
_0026
Dshi_0027: Dshi_0027 - Alkane 1-monooxygenase (RefSeq), at 30,653 to 31,807
_0027
Dshi_0028: Dshi_0028 - A/G-specific adenine glycosylase (RefSeq), at 31,815 to 32,909
_0028
Position (kb) 30
31
32 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 29.667 kb on + strand, within Dshi_0025 at 29.723 kb on + strand, within Dshi_0025 at 29.731 kb on - strand at 29.821 kb on - strand at 29.822 kb on - strand at 29.838 kb on - strand at 29.996 kb on + strand, within Dshi_0026 at 30.039 kb on - strand, within Dshi_0026 at 30.258 kb on - strand, within Dshi_0026 at 30.314 kb on + strand, within Dshi_0026 at 30.337 kb on - strand, within Dshi_0026 at 30.341 kb on + strand, within Dshi_0026 at 30.341 kb on + strand, within Dshi_0026 at 30.351 kb on + strand, within Dshi_0026 at 30.395 kb on + strand, within Dshi_0026 at 30.403 kb on - strand, within Dshi_0026 at 30.419 kb on + strand, within Dshi_0026 at 30.703 kb on - strand at 30.756 kb on + strand at 30.780 kb on + strand, within Dshi_0027 at 30.811 kb on + strand, within Dshi_0027 at 30.821 kb on + strand, within Dshi_0027 at 30.836 kb on + strand, within Dshi_0027 at 30.837 kb on + strand, within Dshi_0027 at 30.857 kb on - strand, within Dshi_0027 at 30.920 kb on + strand, within Dshi_0027 at 30.982 kb on - strand, within Dshi_0027 at 30.982 kb on - strand, within Dshi_0027 at 31.019 kb on - strand, within Dshi_0027 at 31.102 kb on - strand, within Dshi_0027 at 31.145 kb on + strand, within Dshi_0027 at 31.192 kb on + strand, within Dshi_0027 at 31.197 kb on + strand, within Dshi_0027 at 31.234 kb on + strand, within Dshi_0027 at 31.308 kb on + strand, within Dshi_0027 at 31.335 kb on + strand, within Dshi_0027 at 31.343 kb on - strand, within Dshi_0027 at 31.350 kb on + strand, within Dshi_0027 at 31.377 kb on - strand, within Dshi_0027 at 31.611 kb on + strand, within Dshi_0027 at 31.803 kb on + strand at 31.847 kb on - strand at 31.868 kb on - strand at 31.868 kb on - strand at 31.959 kb on + strand, within Dshi_0028 at 32.097 kb on + strand, within Dshi_0028 at 32.166 kb on - strand, within Dshi_0028 at 32.166 kb on - strand, within Dshi_0028 at 32.249 kb on + strand, within Dshi_0028 at 32.376 kb on - strand, within Dshi_0028 at 32.520 kb on - strand, within Dshi_0028 at 32.629 kb on + strand, within Dshi_0028 at 32.678 kb on + strand, within Dshi_0028
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Benzalkonium Chloride 0.0001 mg/ml remove 29,667 + Dshi_0025 0.78 +2.6 29,723 + Dshi_0025 0.86 +0.8 29,731 - +2.6 29,821 - +1.2 29,822 - -0.6 29,838 - +2.3 29,996 + Dshi_0026 0.17 -1.8 30,039 - Dshi_0026 0.24 +1.0 30,258 - Dshi_0026 0.58 +1.0 30,314 + Dshi_0026 0.66 +2.6 30,337 - Dshi_0026 0.70 +1.8 30,341 + Dshi_0026 0.71 -1.3 30,341 + Dshi_0026 0.71 -1.8 30,351 + Dshi_0026 0.72 +1.0 30,395 + Dshi_0026 0.79 -0.7 30,403 - Dshi_0026 0.80 -2.3 30,419 + Dshi_0026 0.83 -3.2 30,703 - +0.1 30,756 + +3.0 30,780 + Dshi_0027 0.11 -3.1 30,811 + Dshi_0027 0.14 -1.3 30,821 + Dshi_0027 0.15 +2.5 30,836 + Dshi_0027 0.16 +0.2 30,837 + Dshi_0027 0.16 +1.0 30,857 - Dshi_0027 0.18 +0.4 30,920 + Dshi_0027 0.23 +1.0 30,982 - Dshi_0027 0.28 -1.0 30,982 - Dshi_0027 0.28 -2.2 31,019 - Dshi_0027 0.32 +3.3 31,102 - Dshi_0027 0.39 +1.3 31,145 + Dshi_0027 0.43 -1.3 31,192 + Dshi_0027 0.47 +3.3 31,197 + Dshi_0027 0.47 +0.2 31,234 + Dshi_0027 0.50 +1.0 31,308 + Dshi_0027 0.57 +2.2 31,335 + Dshi_0027 0.59 -0.2 31,343 - Dshi_0027 0.60 +2.0 31,350 + Dshi_0027 0.60 +0.9 31,377 - Dshi_0027 0.63 +1.7 31,611 + Dshi_0027 0.83 +1.1 31,803 + +2.0 31,847 - -0.6 31,868 - +1.6 31,868 - -0.2 31,959 + Dshi_0028 0.13 +2.6 32,097 + Dshi_0028 0.26 -2.2 32,166 - Dshi_0028 0.32 -1.3 32,166 - Dshi_0028 0.32 +1.3 32,249 + Dshi_0028 0.40 -1.0 32,376 - Dshi_0028 0.51 +2.8 32,520 - Dshi_0028 0.64 +1.0 32,629 + Dshi_0028 0.74 -1.0 32,678 + Dshi_0028 0.79 -1.8
Or see this region's nucleotide sequence