Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_765 and HP15_766 are separated by 6 nucleotides HP15_766 and HP15_767 are separated by 134 nucleotides
HP15_765: HP15_765 - threonine synthase, at 774,334 to 775,734
_765
HP15_766: HP15_766 - bacterial RecJ exonuclease, at 775,741 to 777,474
_766
HP15_767: HP15_767 - peptide chain release factor 2, at 777,609 to 778,664
_767
Position (kb) 775
776
777
778 Strain fitness (log2 ratio) -1
0
1
2 at 775.767 kb on + strand at 775.832 kb on - strand at 775.879 kb on - strand at 775.915 kb on - strand, within HP15_766 at 775.915 kb on - strand, within HP15_766 at 775.993 kb on - strand, within HP15_766 at 776.000 kb on + strand, within HP15_766 at 776.125 kb on + strand, within HP15_766 at 776.273 kb on + strand, within HP15_766 at 776.330 kb on + strand, within HP15_766 at 776.338 kb on - strand, within HP15_766 at 776.361 kb on + strand, within HP15_766 at 776.624 kb on + strand, within HP15_766 at 776.677 kb on - strand, within HP15_766 at 776.677 kb on - strand, within HP15_766 at 776.807 kb on - strand, within HP15_766 at 776.807 kb on - strand, within HP15_766 at 776.867 kb on + strand, within HP15_766 at 776.879 kb on + strand, within HP15_766 at 776.887 kb on - strand, within HP15_766 at 776.887 kb on - strand, within HP15_766 at 776.887 kb on - strand, within HP15_766 at 776.898 kb on - strand, within HP15_766 at 776.933 kb on + strand, within HP15_766 at 776.940 kb on - strand, within HP15_766 at 777.076 kb on + strand, within HP15_766 at 777.190 kb on - strand, within HP15_766 at 777.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 775,767 + +0.2 775,832 - +1.3 775,879 - -0.7 775,915 - HP15_766 0.10 -0.5 775,915 - HP15_766 0.10 -0.1 775,993 - HP15_766 0.15 -0.5 776,000 + HP15_766 0.15 +0.8 776,125 + HP15_766 0.22 -0.1 776,273 + HP15_766 0.31 +0.0 776,330 + HP15_766 0.34 -0.5 776,338 - HP15_766 0.34 +0.3 776,361 + HP15_766 0.36 +0.0 776,624 + HP15_766 0.51 +0.5 776,677 - HP15_766 0.54 -0.9 776,677 - HP15_766 0.54 +1.1 776,807 - HP15_766 0.61 -0.1 776,807 - HP15_766 0.61 +0.5 776,867 + HP15_766 0.65 +0.8 776,879 + HP15_766 0.66 +0.1 776,887 - HP15_766 0.66 +0.9 776,887 - HP15_766 0.66 +0.6 776,887 - HP15_766 0.66 +0.5 776,898 - HP15_766 0.67 -0.1 776,933 + HP15_766 0.69 +0.9 776,940 - HP15_766 0.69 +0.4 777,076 + HP15_766 0.77 +0.5 777,190 - HP15_766 0.84 +2.3 777,391 - -0.2
Or see this region's nucleotide sequence