Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_720 and HP15_721 are separated by 14 nucleotides HP15_721 and HP15_722 are separated by 224 nucleotides HP15_722 and HP15_723 are separated by 90 nucleotides
HP15_720: HP15_720 - succinylglutamate desuccinylase/aspartoacylase family protein, at 728,614 to 729,678
_720
HP15_721: HP15_721 - protein belonging to uncharacterized protein family UPF0047, at 729,693 to 730,118
_721
HP15_722: HP15_722 - TRAP transporter for fumarate, succinate, L-malate, and 2-oxoglutarate, solute receptor component <small>(from data)</small>, at 730,343 to 731,320
_722
HP15_723: HP15_723 - TRAP transporter for fumarate, succinate, L-malate, and 2-oxoglutarate, fused 4TM/12TM components <small>(from data)</small>, at 731,411 to 733,600
_723
Position (kb) 730
731
732 Strain fitness (log2 ratio) -2
-1
0
1 at 729.427 kb on + strand, within HP15_720 at 729.428 kb on + strand, within HP15_720 at 729.435 kb on - strand, within HP15_720 at 729.481 kb on + strand, within HP15_720 at 729.494 kb on - strand, within HP15_720 at 729.654 kb on + strand at 729.654 kb on + strand at 729.654 kb on + strand at 729.654 kb on + strand at 729.664 kb on - strand at 729.698 kb on - strand at 729.731 kb on + strand at 729.739 kb on + strand, within HP15_721 at 729.783 kb on - strand, within HP15_721 at 729.914 kb on - strand, within HP15_721 at 730.305 kb on - strand at 730.417 kb on - strand at 730.417 kb on - strand at 730.417 kb on - strand at 730.556 kb on + strand, within HP15_722 at 730.564 kb on - strand, within HP15_722 at 730.564 kb on - strand, within HP15_722 at 730.683 kb on - strand, within HP15_722 at 730.739 kb on - strand, within HP15_722 at 730.828 kb on + strand, within HP15_722 at 730.869 kb on + strand, within HP15_722 at 730.877 kb on - strand, within HP15_722 at 730.906 kb on - strand, within HP15_722 at 730.941 kb on - strand, within HP15_722 at 730.962 kb on - strand, within HP15_722 at 731.022 kb on + strand, within HP15_722 at 731.113 kb on + strand, within HP15_722 at 731.339 kb on - strand at 731.341 kb on + strand at 731.445 kb on + strand at 731.489 kb on - strand at 731.551 kb on + strand at 731.551 kb on + strand at 731.731 kb on - strand, within HP15_723 at 731.731 kb on - strand, within HP15_723 at 731.834 kb on - strand, within HP15_723 at 731.876 kb on + strand, within HP15_723 at 731.892 kb on + strand, within HP15_723 at 731.900 kb on - strand, within HP15_723 at 731.939 kb on - strand, within HP15_723 at 732.062 kb on + strand, within HP15_723 at 732.097 kb on - strand, within HP15_723 at 732.124 kb on - strand, within HP15_723 at 732.208 kb on + strand, within HP15_723 at 732.216 kb on - strand, within HP15_723 at 732.216 kb on - strand, within HP15_723 at 732.216 kb on - strand, within HP15_723 at 732.228 kb on + strand, within HP15_723 at 732.300 kb on + strand, within HP15_723
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 729,427 + HP15_720 0.76 +0.3 729,428 + HP15_720 0.76 +0.2 729,435 - HP15_720 0.77 +1.3 729,481 + HP15_720 0.81 -0.5 729,494 - HP15_720 0.83 -0.4 729,654 + +1.4 729,654 + +0.6 729,654 + +0.4 729,654 + +0.1 729,664 - -0.5 729,698 - +0.4 729,731 + +0.6 729,739 + HP15_721 0.11 -1.6 729,783 - HP15_721 0.21 +0.1 729,914 - HP15_721 0.52 +0.1 730,305 - -1.4 730,417 - -0.7 730,417 - -1.1 730,417 - +0.4 730,556 + HP15_722 0.22 +0.2 730,564 - HP15_722 0.23 -0.8 730,564 - HP15_722 0.23 -1.3 730,683 - HP15_722 0.35 -0.0 730,739 - HP15_722 0.40 -1.0 730,828 + HP15_722 0.50 -0.9 730,869 + HP15_722 0.54 -1.6 730,877 - HP15_722 0.55 -1.1 730,906 - HP15_722 0.58 +1.0 730,941 - HP15_722 0.61 -0.8 730,962 - HP15_722 0.63 -1.1 731,022 + HP15_722 0.69 -0.0 731,113 + HP15_722 0.79 -0.5 731,339 - -0.9 731,341 + -2.3 731,445 + -1.9 731,489 - -2.1 731,551 + -0.9 731,551 + +0.4 731,731 - HP15_723 0.15 -1.3 731,731 - HP15_723 0.15 -1.4 731,834 - HP15_723 0.19 -0.5 731,876 + HP15_723 0.21 +0.0 731,892 + HP15_723 0.22 -0.7 731,900 - HP15_723 0.22 -0.8 731,939 - HP15_723 0.24 +0.3 732,062 + HP15_723 0.30 -1.1 732,097 - HP15_723 0.31 -0.6 732,124 - HP15_723 0.33 -0.4 732,208 + HP15_723 0.36 +0.1 732,216 - HP15_723 0.37 -1.6 732,216 - HP15_723 0.37 -1.4 732,216 - HP15_723 0.37 -0.4 732,228 + HP15_723 0.37 -1.7 732,300 + HP15_723 0.41 +1.2
Or see this region's nucleotide sequence