Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_670 and HP15_671 overlap by 23 nucleotides HP15_671 and HP15_672 overlap by 8 nucleotides HP15_672 and HP15_673 are separated by 3 nucleotides
HP15_670: HP15_670 - peptidase U35, phage prohead, at 688,489 to 690,591
_670
HP15_671: HP15_671 - phage portal protein, lambda family, at 690,569 to 692,146
_671
HP15_672: HP15_672 - conserved hypothetical protein, at 692,139 to 692,681
_672
HP15_673: HP15_673 - phage terminase GpA, at 692,685 to 694,646
_673
Position (kb) 690
691
692
693 Strain fitness (log2 ratio) -2
-1
0
1
2 at 689.651 kb on - strand, within HP15_670 at 689.731 kb on + strand, within HP15_670 at 689.768 kb on + strand, within HP15_670 at 689.776 kb on - strand, within HP15_670 at 689.831 kb on + strand, within HP15_670 at 689.846 kb on + strand, within HP15_670 at 689.854 kb on - strand, within HP15_670 at 690.048 kb on - strand, within HP15_670 at 690.054 kb on - strand, within HP15_670 at 690.147 kb on + strand, within HP15_670 at 690.147 kb on + strand, within HP15_670 at 690.147 kb on + strand, within HP15_670 at 690.155 kb on - strand, within HP15_670 at 690.170 kb on - strand, within HP15_670 at 690.175 kb on - strand, within HP15_670 at 690.230 kb on - strand, within HP15_670 at 690.313 kb on + strand, within HP15_670 at 690.364 kb on - strand, within HP15_670 at 690.533 kb on + strand at 690.652 kb on - strand at 690.734 kb on - strand, within HP15_671 at 690.754 kb on + strand, within HP15_671 at 690.820 kb on + strand, within HP15_671 at 690.921 kb on + strand, within HP15_671 at 690.921 kb on + strand, within HP15_671 at 690.929 kb on - strand, within HP15_671 at 690.931 kb on + strand, within HP15_671 at 690.961 kb on - strand, within HP15_671 at 691.026 kb on + strand, within HP15_671 at 691.080 kb on - strand, within HP15_671 at 691.085 kb on - strand, within HP15_671 at 691.203 kb on - strand, within HP15_671 at 691.210 kb on + strand, within HP15_671 at 691.226 kb on - strand, within HP15_671 at 691.253 kb on + strand, within HP15_671 at 691.254 kb on - strand, within HP15_671 at 691.358 kb on + strand, within HP15_671 at 691.374 kb on + strand, within HP15_671 at 691.413 kb on - strand, within HP15_671 at 691.624 kb on + strand, within HP15_671 at 691.827 kb on + strand, within HP15_671 at 691.827 kb on + strand, within HP15_671 at 691.827 kb on + strand, within HP15_671 at 691.891 kb on - strand, within HP15_671 at 691.972 kb on + strand, within HP15_671 at 692.019 kb on + strand at 692.019 kb on + strand at 692.027 kb on - strand at 692.027 kb on - strand at 692.092 kb on + strand at 692.150 kb on + strand at 692.157 kb on - strand at 692.204 kb on - strand, within HP15_672 at 692.284 kb on - strand, within HP15_672 at 692.301 kb on + strand, within HP15_672 at 692.309 kb on - strand, within HP15_672 at 692.503 kb on + strand, within HP15_672 at 692.503 kb on + strand, within HP15_672 at 692.506 kb on - strand, within HP15_672 at 692.540 kb on - strand, within HP15_672 at 692.540 kb on - strand, within HP15_672 at 692.595 kb on - strand, within HP15_672 at 692.848 kb on + strand at 692.856 kb on - strand at 692.856 kb on - strand at 692.856 kb on - strand at 692.970 kb on - strand, within HP15_673 at 693.044 kb on + strand, within HP15_673 at 693.052 kb on - strand, within HP15_673
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 689,651 - HP15_670 0.55 -0.3 689,731 + HP15_670 0.59 -0.3 689,768 + HP15_670 0.61 -0.1 689,776 - HP15_670 0.61 -0.3 689,831 + HP15_670 0.64 +0.5 689,846 + HP15_670 0.65 +0.2 689,854 - HP15_670 0.65 -2.2 690,048 - HP15_670 0.74 +0.5 690,054 - HP15_670 0.74 +0.6 690,147 + HP15_670 0.79 -0.0 690,147 + HP15_670 0.79 -0.3 690,147 + HP15_670 0.79 -0.1 690,155 - HP15_670 0.79 -0.1 690,170 - HP15_670 0.80 +0.4 690,175 - HP15_670 0.80 -0.4 690,230 - HP15_670 0.83 +0.5 690,313 + HP15_670 0.87 +0.1 690,364 - HP15_670 0.89 -0.3 690,533 + +1.0 690,652 - +0.9 690,734 - HP15_671 0.10 -2.3 690,754 + HP15_671 0.12 -0.2 690,820 + HP15_671 0.16 +1.0 690,921 + HP15_671 0.22 +1.3 690,921 + HP15_671 0.22 +0.6 690,929 - HP15_671 0.23 -0.3 690,931 + HP15_671 0.23 -0.1 690,961 - HP15_671 0.25 +0.7 691,026 + HP15_671 0.29 +0.9 691,080 - HP15_671 0.32 -0.3 691,085 - HP15_671 0.33 +2.1 691,203 - HP15_671 0.40 +2.6 691,210 + HP15_671 0.41 -0.0 691,226 - HP15_671 0.42 +0.8 691,253 + HP15_671 0.43 -0.2 691,254 - HP15_671 0.43 +0.2 691,358 + HP15_671 0.50 -0.1 691,374 + HP15_671 0.51 +1.5 691,413 - HP15_671 0.53 +1.1 691,624 + HP15_671 0.67 +1.3 691,827 + HP15_671 0.80 +1.0 691,827 + HP15_671 0.80 +1.4 691,827 + HP15_671 0.80 -0.3 691,891 - HP15_671 0.84 +0.6 691,972 + HP15_671 0.89 -1.2 692,019 + -0.5 692,019 + -0.1 692,027 - +1.1 692,027 - -1.7 692,092 + -0.2 692,150 + -0.2 692,157 - +0.2 692,204 - HP15_672 0.12 -0.2 692,284 - HP15_672 0.27 +0.1 692,301 + HP15_672 0.30 -0.2 692,309 - HP15_672 0.31 -0.3 692,503 + HP15_672 0.67 -0.5 692,503 + HP15_672 0.67 -0.3 692,506 - HP15_672 0.68 +1.2 692,540 - HP15_672 0.74 +1.1 692,540 - HP15_672 0.74 +0.1 692,595 - HP15_672 0.84 +1.1 692,848 + +1.1 692,856 - -2.0 692,856 - -0.1 692,856 - +0.0 692,970 - HP15_673 0.15 -0.7 693,044 + HP15_673 0.18 +1.1 693,052 - HP15_673 0.19 -1.7
Or see this region's nucleotide sequence