Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_p187g25 and HP15_p187g26 are separated by 277 nucleotides HP15_p187g26 and HP15_p187g27 are separated by 124 nucleotides HP15_p187g27 and HP15_p187g28 are separated by 129 nucleotides HP15_p187g28 and HP15_p187g29 are separated by 34 nucleotides HP15_p187g29 and HP15_p187g30 are separated by 125 nucleotides HP15_p187g30 and HP15_p187g31 are separated by 214 nucleotides
HP15_p187g25: HP15_p187g25 - membrane protein, at 20,626 to 20,931
_p187g25
HP15_p187g26: HP15_p187g26 - hypothetical protein, at 21,209 to 21,370
_p187g26
HP15_p187g27: HP15_p187g27 - hypothetical protein, at 21,495 to 21,662
_p187g27
HP15_p187g28: HP15_p187g28 - hypothetical protein, at 21,792 to 21,929
_p187g28
HP15_p187g29: HP15_p187g29 - hypothetical protein, at 21,964 to 22,341
_p187g29
HP15_p187g30: HP15_p187g30 - conserved hypothetical protein, membrane, at 22,467 to 22,688
_p187g30
HP15_p187g31: HP15_p187g31 - protein containing nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase domain, at 22,903 to 24,063
_p187g31
Position (kb)
21
22 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 20.825 kb on + strand, within HP15_p187g25 at 20.862 kb on + strand, within HP15_p187g25 at 20.948 kb on - strand at 21.025 kb on - strand at 21.026 kb on + strand at 21.051 kb on + strand at 21.141 kb on + strand at 21.172 kb on + strand at 21.175 kb on - strand at 21.180 kb on - strand at 21.196 kb on - strand at 21.263 kb on - strand, within HP15_p187g26 at 21.265 kb on + strand, within HP15_p187g26 at 21.293 kb on + strand, within HP15_p187g26 at 21.311 kb on - strand, within HP15_p187g26 at 21.351 kb on + strand, within HP15_p187g26 at 21.356 kb on + strand at 21.364 kb on - strand at 21.382 kb on - strand at 21.431 kb on - strand at 21.518 kb on - strand, within HP15_p187g27 at 21.551 kb on + strand, within HP15_p187g27 at 21.606 kb on + strand, within HP15_p187g27 at 21.626 kb on + strand, within HP15_p187g27 at 21.626 kb on + strand, within HP15_p187g27 at 21.646 kb on + strand at 21.646 kb on + strand at 21.646 kb on + strand at 21.664 kb on - strand at 21.690 kb on + strand at 21.698 kb on - strand at 21.713 kb on + strand at 21.713 kb on + strand at 21.721 kb on - strand at 21.731 kb on - strand at 21.758 kb on + strand at 21.829 kb on - strand, within HP15_p187g28 at 21.829 kb on - strand, within HP15_p187g28 at 21.847 kb on + strand, within HP15_p187g28 at 21.847 kb on + strand, within HP15_p187g28 at 21.847 kb on + strand, within HP15_p187g28 at 21.855 kb on - strand, within HP15_p187g28 at 21.866 kb on - strand, within HP15_p187g28 at 21.928 kb on + strand at 21.928 kb on + strand at 21.999 kb on + strand at 21.999 kb on + strand at 22.007 kb on - strand, within HP15_p187g29 at 22.007 kb on - strand, within HP15_p187g29 at 22.021 kb on - strand, within HP15_p187g29 at 22.021 kb on - strand, within HP15_p187g29 at 22.057 kb on - strand, within HP15_p187g29 at 22.120 kb on + strand, within HP15_p187g29 at 22.223 kb on + strand, within HP15_p187g29 at 22.223 kb on + strand, within HP15_p187g29 at 22.225 kb on + strand, within HP15_p187g29 at 22.231 kb on - strand, within HP15_p187g29 at 22.260 kb on + strand, within HP15_p187g29 at 22.260 kb on + strand, within HP15_p187g29 at 22.260 kb on + strand, within HP15_p187g29 at 22.268 kb on - strand, within HP15_p187g29 at 22.268 kb on - strand, within HP15_p187g29 at 22.268 kb on - strand, within HP15_p187g29 at 22.313 kb on + strand at 22.313 kb on + strand at 22.313 kb on + strand at 22.321 kb on - strand at 22.321 kb on - strand at 22.321 kb on - strand at 22.321 kb on - strand at 22.321 kb on - strand at 22.326 kb on - strand at 22.331 kb on - strand at 22.413 kb on - strand at 22.413 kb on - strand at 22.478 kb on - strand at 22.483 kb on + strand at 22.525 kb on - strand, within HP15_p187g30 at 22.525 kb on - strand, within HP15_p187g30 at 22.617 kb on + strand, within HP15_p187g30 at 22.617 kb on + strand, within HP15_p187g30 at 22.632 kb on + strand, within HP15_p187g30 at 22.632 kb on + strand, within HP15_p187g30 at 22.632 kb on + strand, within HP15_p187g30 at 22.640 kb on - strand, within HP15_p187g30 at 22.651 kb on + strand, within HP15_p187g30 at 22.659 kb on - strand, within HP15_p187g30 at 22.735 kb on - strand at 22.737 kb on + strand at 22.737 kb on + strand at 22.744 kb on + strand at 22.744 kb on + strand at 22.744 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.749 kb on + strand at 22.757 kb on - strand at 22.757 kb on - strand at 22.757 kb on - strand at 22.757 kb on - strand at 22.757 kb on - strand at 22.757 kb on - strand at 22.880 kb on - strand at 22.886 kb on + strand at 22.894 kb on - strand at 22.894 kb on - strand at 22.904 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 20,825 + HP15_p187g25 0.65 -0.2 20,862 + HP15_p187g25 0.77 -0.1 20,948 - -0.7 21,025 - -0.2 21,026 + +0.9 21,051 + -0.0 21,141 + +0.4 21,172 + -0.3 21,175 - +0.6 21,180 - -0.2 21,196 - -0.2 21,263 - HP15_p187g26 0.33 +0.1 21,265 + HP15_p187g26 0.35 +1.9 21,293 + HP15_p187g26 0.52 -0.3 21,311 - HP15_p187g26 0.63 -2.6 21,351 + HP15_p187g26 0.88 -0.9 21,356 + -1.5 21,364 - -0.5 21,382 - -0.6 21,431 - -0.8 21,518 - HP15_p187g27 0.14 +0.0 21,551 + HP15_p187g27 0.33 +1.3 21,606 + HP15_p187g27 0.66 +0.3 21,626 + HP15_p187g27 0.78 -0.0 21,626 + HP15_p187g27 0.78 +0.2 21,646 + -0.2 21,646 + -0.7 21,646 + -2.4 21,664 - +0.6 21,690 + +0.5 21,698 - +0.3 21,713 + -0.7 21,713 + +0.1 21,721 - +1.4 21,731 - -0.4 21,758 + +1.1 21,829 - HP15_p187g28 0.27 -1.7 21,829 - HP15_p187g28 0.27 -1.0 21,847 + HP15_p187g28 0.40 -1.5 21,847 + HP15_p187g28 0.40 -0.4 21,847 + HP15_p187g28 0.40 -0.2 21,855 - HP15_p187g28 0.46 +0.4 21,866 - HP15_p187g28 0.54 -0.4 21,928 + +0.7 21,928 + +0.6 21,999 + +0.5 21,999 + -0.0 22,007 - HP15_p187g29 0.11 +0.6 22,007 - HP15_p187g29 0.11 +0.1 22,021 - HP15_p187g29 0.15 -0.3 22,021 - HP15_p187g29 0.15 +0.6 22,057 - HP15_p187g29 0.25 -0.0 22,120 + HP15_p187g29 0.41 +0.5 22,223 + HP15_p187g29 0.69 +0.3 22,223 + HP15_p187g29 0.69 +1.4 22,225 + HP15_p187g29 0.69 +1.1 22,231 - HP15_p187g29 0.71 -0.9 22,260 + HP15_p187g29 0.78 +0.6 22,260 + HP15_p187g29 0.78 +0.2 22,260 + HP15_p187g29 0.78 +0.7 22,268 - HP15_p187g29 0.80 -0.2 22,268 - HP15_p187g29 0.80 -0.4 22,268 - HP15_p187g29 0.80 -1.6 22,313 + +1.1 22,313 + -0.1 22,313 + +0.6 22,321 - +0.4 22,321 - +0.4 22,321 - +1.2 22,321 - +1.9 22,321 - -1.8 22,326 - +0.0 22,331 - -0.0 22,413 - +0.6 22,413 - -0.4 22,478 - -0.2 22,483 + -0.1 22,525 - HP15_p187g30 0.26 -0.3 22,525 - HP15_p187g30 0.26 -1.8 22,617 + HP15_p187g30 0.68 -0.5 22,617 + HP15_p187g30 0.68 -0.3 22,632 + HP15_p187g30 0.74 +0.1 22,632 + HP15_p187g30 0.74 +0.1 22,632 + HP15_p187g30 0.74 -1.6 22,640 - HP15_p187g30 0.78 +0.3 22,651 + HP15_p187g30 0.83 -0.5 22,659 - HP15_p187g30 0.86 -1.3 22,735 - +1.8 22,737 + +0.5 22,737 + -0.2 22,744 + -0.2 22,744 + +0.4 22,744 + +1.2 22,749 + +0.9 22,749 + +1.4 22,749 + -0.1 22,749 + +0.1 22,749 + +0.4 22,749 + +1.2 22,749 + +0.2 22,749 + +0.6 22,749 + +0.1 22,757 - +0.3 22,757 - +0.0 22,757 - -0.1 22,757 - -0.3 22,757 - -0.7 22,757 - -0.5 22,880 - -0.7 22,886 + -0.2 22,894 - -0.2 22,894 - +0.4 22,904 - -0.1
Or see this region's nucleotide sequence