Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_p42g15 and HP15_p42g16 are separated by 41 nucleotides HP15_p42g16 and HP15_p42g17 are separated by 21 nucleotides
HP15_p42g15: HP15_p42g15 - KfrA protein, at 9,615 to 10,646
_p42g15
HP15_p42g16: HP15_p42g16 - conjugal transfer protein TraO, at 10,688 to 11,152
_p42g16
HP15_p42g17: HP15_p42g17 - traN-like protein, at 11,174 to 12,502
_p42g17
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 9.695 kb on + strand at 9.702 kb on + strand at 9.702 kb on + strand at 9.702 kb on + strand at 9.710 kb on - strand at 9.710 kb on - strand at 9.710 kb on - strand at 9.731 kb on - strand, within HP15_p42g15 at 9.758 kb on + strand, within HP15_p42g15 at 9.780 kb on - strand, within HP15_p42g15 at 9.827 kb on + strand, within HP15_p42g15 at 9.828 kb on - strand, within HP15_p42g15 at 9.852 kb on + strand, within HP15_p42g15 at 9.903 kb on - strand, within HP15_p42g15 at 10.014 kb on + strand, within HP15_p42g15 at 10.043 kb on + strand, within HP15_p42g15 at 10.066 kb on - strand, within HP15_p42g15 at 10.145 kb on - strand, within HP15_p42g15 at 10.189 kb on + strand, within HP15_p42g15 at 10.199 kb on - strand, within HP15_p42g15 at 10.244 kb on + strand, within HP15_p42g15 at 10.253 kb on - strand, within HP15_p42g15 at 10.298 kb on + strand, within HP15_p42g15 at 10.412 kb on + strand, within HP15_p42g15 at 10.412 kb on + strand, within HP15_p42g15 at 10.548 kb on + strand at 10.585 kb on + strand at 10.633 kb on - strand at 10.710 kb on + strand at 10.789 kb on + strand, within HP15_p42g16 at 10.789 kb on + strand, within HP15_p42g16 at 10.856 kb on + strand, within HP15_p42g16 at 10.864 kb on - strand, within HP15_p42g16 at 10.990 kb on + strand, within HP15_p42g16 at 11.106 kb on + strand at 11.106 kb on - strand at 11.188 kb on + strand at 11.283 kb on - strand at 11.363 kb on - strand, within HP15_p42g17 at 11.384 kb on + strand, within HP15_p42g17 at 11.384 kb on + strand, within HP15_p42g17 at 11.472 kb on + strand, within HP15_p42g17 at 11.480 kb on - strand, within HP15_p42g17 at 11.490 kb on + strand, within HP15_p42g17 at 11.503 kb on - strand, within HP15_p42g17 at 11.534 kb on + strand, within HP15_p42g17 at 11.537 kb on - strand, within HP15_p42g17 at 11.571 kb on + strand, within HP15_p42g17 at 11.689 kb on - strand, within HP15_p42g17 at 11.722 kb on + strand, within HP15_p42g17 at 11.966 kb on + strand, within HP15_p42g17 at 11.982 kb on + strand, within HP15_p42g17 at 11.990 kb on - strand, within HP15_p42g17 at 12.022 kb on + strand, within HP15_p42g17 at 12.050 kb on - strand, within HP15_p42g17 at 12.146 kb on - strand, within HP15_p42g17
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 9,695 + +0.3 9,702 + +0.1 9,702 + +1.5 9,702 + +0.9 9,710 - +0.4 9,710 - +0.9 9,710 - -1.7 9,731 - HP15_p42g15 0.11 -0.3 9,758 + HP15_p42g15 0.14 +0.6 9,780 - HP15_p42g15 0.16 +0.1 9,827 + HP15_p42g15 0.21 +0.6 9,828 - HP15_p42g15 0.21 +0.8 9,852 + HP15_p42g15 0.23 -0.1 9,903 - HP15_p42g15 0.28 -0.0 10,014 + HP15_p42g15 0.39 +0.5 10,043 + HP15_p42g15 0.41 -0.4 10,066 - HP15_p42g15 0.44 -0.9 10,145 - HP15_p42g15 0.51 -0.0 10,189 + HP15_p42g15 0.56 -0.1 10,199 - HP15_p42g15 0.57 -0.4 10,244 + HP15_p42g15 0.61 +1.3 10,253 - HP15_p42g15 0.62 -0.6 10,298 + HP15_p42g15 0.66 +0.8 10,412 + HP15_p42g15 0.77 -0.6 10,412 + HP15_p42g15 0.77 +0.7 10,548 + +0.6 10,585 + +1.0 10,633 - -1.2 10,710 + +0.9 10,789 + HP15_p42g16 0.22 -0.1 10,789 + HP15_p42g16 0.22 -0.8 10,856 + HP15_p42g16 0.36 -0.9 10,864 - HP15_p42g16 0.38 -0.7 10,990 + HP15_p42g16 0.65 -3.9 11,106 + -0.7 11,106 - -1.2 11,188 + +0.6 11,283 - -0.7 11,363 - HP15_p42g17 0.14 +0.7 11,384 + HP15_p42g17 0.16 +0.2 11,384 + HP15_p42g17 0.16 -0.2 11,472 + HP15_p42g17 0.22 +0.9 11,480 - HP15_p42g17 0.23 -0.2 11,490 + HP15_p42g17 0.24 +0.5 11,503 - HP15_p42g17 0.25 -0.9 11,534 + HP15_p42g17 0.27 +1.4 11,537 - HP15_p42g17 0.27 -0.6 11,571 + HP15_p42g17 0.30 -0.6 11,689 - HP15_p42g17 0.39 -1.5 11,722 + HP15_p42g17 0.41 +0.8 11,966 + HP15_p42g17 0.60 -1.1 11,982 + HP15_p42g17 0.61 -0.6 11,990 - HP15_p42g17 0.61 -0.1 12,022 + HP15_p42g17 0.64 +0.7 12,050 - HP15_p42g17 0.66 -1.2 12,146 - HP15_p42g17 0.73 +0.0
Or see this region's nucleotide sequence