Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4128 and HP15_4129 are separated by 26 nucleotides HP15_4129 and HP15_4130 overlap by 4 nucleotides HP15_4130 and HP15_4131 are separated by 12 nucleotides HP15_4131 and HP15_4132 are separated by 32 nucleotides
HP15_4128: HP15_4128 - molybdopterin biosynthesis protein MoeA-like protein, at 4,363,739 to 4,364,986
_4128
HP15_4129: HP15_4129 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 4,365,013 to 4,365,504
_4129
HP15_4130: HP15_4130 - molybdopterin-guanine dinucleotide biosynthesis protein A, at 4,365,501 to 4,366,088
_4130
HP15_4131: HP15_4131 - formate dehydrogenase family accessory protein FdhD, at 4,366,101 to 4,366,970
_4131
HP15_4132: HP15_4132 - hypothetical protein, at 4,367,003 to 4,367,113
_4132
Position (kb)
4365
4366
4367 Strain fitness (log2 ratio)
-1
0
1 at 4364.517 kb on + strand, within HP15_4128 at 4364.617 kb on + strand, within HP15_4128 at 4364.681 kb on + strand, within HP15_4128 at 4364.689 kb on - strand, within HP15_4128 at 4364.759 kb on + strand, within HP15_4128 at 4364.774 kb on + strand, within HP15_4128 at 4364.836 kb on + strand, within HP15_4128 at 4364.855 kb on + strand, within HP15_4128 at 4364.881 kb on + strand at 4364.887 kb on - strand at 4364.946 kb on + strand at 4364.964 kb on - strand at 4365.148 kb on + strand, within HP15_4129 at 4365.222 kb on + strand, within HP15_4129 at 4365.227 kb on - strand, within HP15_4129 at 4365.270 kb on + strand, within HP15_4129 at 4365.285 kb on - strand, within HP15_4129 at 4365.290 kb on + strand, within HP15_4129 at 4365.353 kb on - strand, within HP15_4129 at 4365.456 kb on + strand at 4365.520 kb on + strand at 4365.528 kb on - strand at 4365.533 kb on - strand at 4365.674 kb on + strand, within HP15_4130 at 4365.682 kb on - strand, within HP15_4130 at 4365.773 kb on + strand, within HP15_4130 at 4365.789 kb on - strand, within HP15_4130 at 4366.106 kb on - strand at 4366.152 kb on - strand at 4366.169 kb on - strand at 4366.190 kb on - strand, within HP15_4131 at 4366.232 kb on + strand, within HP15_4131 at 4366.305 kb on + strand, within HP15_4131 at 4366.306 kb on + strand, within HP15_4131 at 4366.346 kb on + strand, within HP15_4131 at 4366.346 kb on + strand, within HP15_4131 at 4366.354 kb on + strand, within HP15_4131 at 4366.359 kb on - strand, within HP15_4131 at 4366.395 kb on + strand, within HP15_4131 at 4366.465 kb on + strand, within HP15_4131 at 4366.465 kb on + strand, within HP15_4131 at 4366.482 kb on + strand, within HP15_4131 at 4366.483 kb on - strand, within HP15_4131 at 4366.552 kb on + strand, within HP15_4131 at 4366.565 kb on - strand, within HP15_4131 at 4366.575 kb on - strand, within HP15_4131 at 4366.690 kb on - strand, within HP15_4131 at 4366.820 kb on - strand, within HP15_4131 at 4367.027 kb on + strand, within HP15_4132 at 4367.037 kb on - strand, within HP15_4132
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 4,364,517 + HP15_4128 0.62 +0.0 4,364,617 + HP15_4128 0.70 +0.0 4,364,681 + HP15_4128 0.75 -0.3 4,364,689 - HP15_4128 0.76 -0.3 4,364,759 + HP15_4128 0.82 +0.4 4,364,774 + HP15_4128 0.83 +1.6 4,364,836 + HP15_4128 0.88 +0.7 4,364,855 + HP15_4128 0.89 -0.0 4,364,881 + -0.3 4,364,887 - +0.3 4,364,946 + +0.3 4,364,964 - -0.5 4,365,148 + HP15_4129 0.27 -0.1 4,365,222 + HP15_4129 0.42 +0.0 4,365,227 - HP15_4129 0.43 +0.6 4,365,270 + HP15_4129 0.52 -0.3 4,365,285 - HP15_4129 0.55 -1.0 4,365,290 + HP15_4129 0.56 +1.0 4,365,353 - HP15_4129 0.69 -0.4 4,365,456 + +0.6 4,365,520 + -0.3 4,365,528 - -0.3 4,365,533 - -0.9 4,365,674 + HP15_4130 0.29 -0.6 4,365,682 - HP15_4130 0.31 +0.0 4,365,773 + HP15_4130 0.46 +0.1 4,365,789 - HP15_4130 0.49 -0.7 4,366,106 - +0.3 4,366,152 - +0.2 4,366,169 - +1.2 4,366,190 - HP15_4131 0.10 -0.5 4,366,232 + HP15_4131 0.15 -0.6 4,366,305 + HP15_4131 0.23 -0.1 4,366,306 + HP15_4131 0.24 +0.6 4,366,346 + HP15_4131 0.28 -0.2 4,366,346 + HP15_4131 0.28 +0.2 4,366,354 + HP15_4131 0.29 -0.6 4,366,359 - HP15_4131 0.30 -0.2 4,366,395 + HP15_4131 0.34 +0.6 4,366,465 + HP15_4131 0.42 +0.3 4,366,465 + HP15_4131 0.42 +0.5 4,366,482 + HP15_4131 0.44 -0.1 4,366,483 - HP15_4131 0.44 +1.0 4,366,552 + HP15_4131 0.52 -0.7 4,366,565 - HP15_4131 0.53 -0.1 4,366,575 - HP15_4131 0.54 -0.9 4,366,690 - HP15_4131 0.68 +0.2 4,366,820 - HP15_4131 0.83 +0.7 4,367,027 + HP15_4132 0.22 -0.3 4,367,037 - HP15_4132 0.31 +0.1
Or see this region's nucleotide sequence