Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4076 and HP15_4077 are separated by 45 nucleotides HP15_4077 and HP15_4078 are separated by 32 nucleotides HP15_4078 and HP15_4079 overlap by 4 nucleotides
HP15_4076: HP15_4076 - TRAP dicarboxylate transporter, DctP subunit, at 4,309,735 to 4,310,859
_4076
HP15_4077: HP15_4077 - conserved hypothetical protein, at 4,310,905 to 4,311,531
_4077
HP15_4078: HP15_4078 - glutathione peroxidase, at 4,311,564 to 4,312,136
_4078
HP15_4079: HP15_4079 - flavin dependent oxidoreductase, at 4,312,133 to 4,313,116
_4079
Position (kb) 4310
4311
4312 Strain fitness (log2 ratio) -1
0
1 at 4309.952 kb on - strand, within HP15_4076 at 4309.978 kb on + strand, within HP15_4076 at 4310.157 kb on - strand, within HP15_4076 at 4310.159 kb on + strand, within HP15_4076 at 4310.162 kb on + strand, within HP15_4076 at 4310.170 kb on - strand, within HP15_4076 at 4310.170 kb on - strand, within HP15_4076 at 4310.179 kb on + strand, within HP15_4076 at 4310.182 kb on - strand, within HP15_4076 at 4310.188 kb on + strand, within HP15_4076 at 4310.244 kb on - strand, within HP15_4076 at 4310.351 kb on + strand, within HP15_4076 at 4310.366 kb on - strand, within HP15_4076 at 4310.439 kb on + strand, within HP15_4076 at 4310.475 kb on + strand, within HP15_4076 at 4310.501 kb on - strand, within HP15_4076 at 4310.506 kb on + strand, within HP15_4076 at 4310.588 kb on + strand, within HP15_4076 at 4310.591 kb on - strand, within HP15_4076 at 4310.698 kb on + strand, within HP15_4076 at 4310.710 kb on + strand, within HP15_4076 at 4310.721 kb on + strand, within HP15_4076 at 4310.876 kb on - strand at 4311.056 kb on + strand, within HP15_4077 at 4311.096 kb on + strand, within HP15_4077 at 4311.101 kb on + strand, within HP15_4077 at 4311.118 kb on + strand, within HP15_4077 at 4311.308 kb on + strand, within HP15_4077 at 4311.445 kb on + strand, within HP15_4077 at 4311.499 kb on + strand at 4311.504 kb on + strand at 4311.504 kb on + strand at 4311.507 kb on - strand at 4311.519 kb on - strand at 4311.641 kb on + strand, within HP15_4078 at 4311.715 kb on + strand, within HP15_4078 at 4311.723 kb on - strand, within HP15_4078 at 4311.723 kb on - strand, within HP15_4078 at 4311.758 kb on + strand, within HP15_4078 at 4311.792 kb on - strand, within HP15_4078 at 4311.803 kb on + strand, within HP15_4078 at 4312.062 kb on + strand, within HP15_4078 at 4312.082 kb on - strand at 4312.272 kb on - strand, within HP15_4079 at 4312.305 kb on - strand, within HP15_4079 at 4312.370 kb on + strand, within HP15_4079 at 4312.493 kb on + strand, within HP15_4079 at 4312.506 kb on - strand, within HP15_4079
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 4,309,952 - HP15_4076 0.19 -0.9 4,309,978 + HP15_4076 0.22 -0.3 4,310,157 - HP15_4076 0.38 +1.0 4,310,159 + HP15_4076 0.38 -0.5 4,310,162 + HP15_4076 0.38 -0.2 4,310,170 - HP15_4076 0.39 +0.3 4,310,170 - HP15_4076 0.39 +0.8 4,310,179 + HP15_4076 0.39 +1.2 4,310,182 - HP15_4076 0.40 -0.6 4,310,188 + HP15_4076 0.40 -0.4 4,310,244 - HP15_4076 0.45 -0.9 4,310,351 + HP15_4076 0.55 +0.5 4,310,366 - HP15_4076 0.56 -0.4 4,310,439 + HP15_4076 0.63 +0.7 4,310,475 + HP15_4076 0.66 +0.1 4,310,501 - HP15_4076 0.68 +0.9 4,310,506 + HP15_4076 0.69 +0.6 4,310,588 + HP15_4076 0.76 -0.4 4,310,591 - HP15_4076 0.76 -0.5 4,310,698 + HP15_4076 0.86 +0.8 4,310,710 + HP15_4076 0.87 -0.5 4,310,721 + HP15_4076 0.88 +0.7 4,310,876 - +1.0 4,311,056 + HP15_4077 0.24 -0.5 4,311,096 + HP15_4077 0.30 -1.3 4,311,101 + HP15_4077 0.31 +0.1 4,311,118 + HP15_4077 0.34 -0.6 4,311,308 + HP15_4077 0.64 +0.6 4,311,445 + HP15_4077 0.86 -1.4 4,311,499 + +0.5 4,311,504 + +0.4 4,311,504 + -0.1 4,311,507 - -0.9 4,311,519 - -0.6 4,311,641 + HP15_4078 0.13 +0.2 4,311,715 + HP15_4078 0.26 +1.2 4,311,723 - HP15_4078 0.28 -0.1 4,311,723 - HP15_4078 0.28 +0.5 4,311,758 + HP15_4078 0.34 -0.6 4,311,792 - HP15_4078 0.40 +0.5 4,311,803 + HP15_4078 0.42 -1.3 4,312,062 + HP15_4078 0.87 -0.6 4,312,082 - -0.7 4,312,272 - HP15_4079 0.14 -0.1 4,312,305 - HP15_4079 0.17 +1.1 4,312,370 + HP15_4079 0.24 +1.0 4,312,493 + HP15_4079 0.37 -0.2 4,312,506 - HP15_4079 0.38 +0.3
Or see this region's nucleotide sequence