Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3845 and HP15_3846 are separated by 43 nucleotides HP15_3846 and HP15_3847 overlap by 4 nucleotides HP15_3847 and HP15_3848 overlap by 7 nucleotides HP15_3848 and HP15_3849 are separated by 254 nucleotides
HP15_3845: HP15_3845 - ADP-ribosylation/crystallin J1-like protein, at 4,071,539 to 4,072,711
_3845
HP15_3846: HP15_3846 - ethyl tert-butyl ether degradation EthD, at 4,072,755 to 4,073,066
_3846
HP15_3847: HP15_3847 - transmembrane transcriptional regulator (anti-Sigma-factor), at 4,073,063 to 4,073,329
_3847
HP15_3848: HP15_3848 - RNA polymerase, sigma-24 subunit, ECF subfamily, at 4,073,323 to 4,073,940
_3848
HP15_3849: HP15_3849 - pyridine nucleotide-disulfide oxidoreductase dimerization region, at 4,074,195 to 4,076,384
_3849
Position (kb)
4073
4074 Strain fitness (log2 ratio)
-1
0
1 at 4072.068 kb on - strand, within HP15_3845 at 4072.116 kb on + strand, within HP15_3845 at 4072.226 kb on + strand, within HP15_3845 at 4072.305 kb on + strand, within HP15_3845 at 4072.424 kb on - strand, within HP15_3845 at 4072.542 kb on - strand, within HP15_3845 at 4072.588 kb on + strand, within HP15_3845 at 4072.588 kb on + strand, within HP15_3845 at 4072.607 kb on + strand at 4072.701 kb on + strand at 4072.706 kb on + strand at 4072.781 kb on - strand at 4072.781 kb on - strand at 4072.811 kb on - strand, within HP15_3846 at 4072.889 kb on + strand, within HP15_3846 at 4072.970 kb on + strand, within HP15_3846 at 4073.015 kb on + strand, within HP15_3846 at 4073.183 kb on + strand, within HP15_3847 at 4073.266 kb on - strand, within HP15_3847 at 4073.408 kb on - strand, within HP15_3848 at 4073.408 kb on - strand, within HP15_3848 at 4073.480 kb on - strand, within HP15_3848 at 4073.494 kb on - strand, within HP15_3848 at 4073.494 kb on - strand, within HP15_3848 at 4073.496 kb on + strand, within HP15_3848 at 4073.506 kb on - strand, within HP15_3848 at 4073.627 kb on + strand, within HP15_3848 at 4073.638 kb on - strand, within HP15_3848 at 4073.666 kb on - strand, within HP15_3848 at 4073.708 kb on - strand, within HP15_3848 at 4073.766 kb on + strand, within HP15_3848 at 4073.897 kb on + strand at 4074.084 kb on + strand at 4074.103 kb on - strand at 4074.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 4,072,068 - HP15_3845 0.45 -0.1 4,072,116 + HP15_3845 0.49 +1.0 4,072,226 + HP15_3845 0.59 -0.1 4,072,305 + HP15_3845 0.65 +0.5 4,072,424 - HP15_3845 0.75 -0.4 4,072,542 - HP15_3845 0.86 -0.4 4,072,588 + HP15_3845 0.89 -0.3 4,072,588 + HP15_3845 0.89 -0.3 4,072,607 + +0.9 4,072,701 + -0.5 4,072,706 + -0.4 4,072,781 - -0.2 4,072,781 - +1.7 4,072,811 - HP15_3846 0.18 -0.4 4,072,889 + HP15_3846 0.43 -1.0 4,072,970 + HP15_3846 0.69 +0.6 4,073,015 + HP15_3846 0.83 +0.6 4,073,183 + HP15_3847 0.45 -0.3 4,073,266 - HP15_3847 0.76 -0.6 4,073,408 - HP15_3848 0.14 +1.2 4,073,408 - HP15_3848 0.14 -0.6 4,073,480 - HP15_3848 0.25 -0.4 4,073,494 - HP15_3848 0.28 +1.2 4,073,494 - HP15_3848 0.28 -0.1 4,073,496 + HP15_3848 0.28 -0.4 4,073,506 - HP15_3848 0.30 +1.0 4,073,627 + HP15_3848 0.49 +0.9 4,073,638 - HP15_3848 0.51 +0.1 4,073,666 - HP15_3848 0.56 -0.2 4,073,708 - HP15_3848 0.62 -1.7 4,073,766 + HP15_3848 0.72 -0.0 4,073,897 + +0.0 4,074,084 + +0.0 4,074,103 - +0.2 4,074,281 - -0.3
Or see this region's nucleotide sequence