Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3822 and HP15_3823 are separated by 238 nucleotides HP15_3823 and HP15_3824 are separated by 52 nucleotides HP15_3824 and HP15_3825 are separated by 123 nucleotides
HP15_3822: HP15_3822 - drug resistance transporter, EmrB/QacA family, at 4,048,000 to 4,049,391
_3822
HP15_3823: HP15_3823 - uncharacterized protein family UPF0016-like protein, at 4,049,630 to 4,050,199
_3823
HP15_3824: HP15_3824 - ErfK/YbiS/YcfS/YnhG family protein, at 4,050,252 to 4,051,298
_3824
HP15_3825: HP15_3825 - NfeD family protein, at 4,051,422 to 4,052,891
_3825
Position (kb)
4050
4051
4052 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4049.294 kb on - strand at 4049.299 kb on - strand at 4049.299 kb on - strand at 4049.385 kb on + strand at 4049.385 kb on + strand at 4049.412 kb on - strand at 4049.443 kb on + strand at 4049.451 kb on - strand at 4049.525 kb on - strand at 4049.784 kb on - strand, within HP15_3823 at 4049.807 kb on + strand, within HP15_3823 at 4049.833 kb on - strand, within HP15_3823 at 4049.895 kb on - strand, within HP15_3823 at 4049.914 kb on + strand, within HP15_3823 at 4049.986 kb on + strand, within HP15_3823 at 4050.035 kb on + strand, within HP15_3823 at 4050.038 kb on - strand, within HP15_3823 at 4050.042 kb on + strand, within HP15_3823 at 4050.077 kb on - strand, within HP15_3823 at 4050.113 kb on + strand, within HP15_3823 at 4050.171 kb on + strand at 4050.171 kb on + strand at 4050.404 kb on - strand, within HP15_3824 at 4050.470 kb on + strand, within HP15_3824 at 4050.478 kb on - strand, within HP15_3824 at 4050.503 kb on + strand, within HP15_3824 at 4050.511 kb on - strand, within HP15_3824 at 4050.661 kb on - strand, within HP15_3824 at 4050.693 kb on + strand, within HP15_3824 at 4050.693 kb on + strand, within HP15_3824 at 4050.763 kb on - strand, within HP15_3824 at 4050.775 kb on - strand, within HP15_3824 at 4050.846 kb on - strand, within HP15_3824 at 4050.980 kb on + strand, within HP15_3824 at 4050.998 kb on - strand, within HP15_3824 at 4051.090 kb on + strand, within HP15_3824 at 4051.109 kb on + strand, within HP15_3824 at 4051.278 kb on + strand at 4051.328 kb on + strand at 4051.328 kb on + strand at 4051.328 kb on + strand at 4051.328 kb on + strand at 4051.328 kb on + strand at 4051.336 kb on - strand at 4051.336 kb on - strand at 4051.336 kb on - strand at 4051.336 kb on - strand at 4051.336 kb on - strand at 4051.396 kb on - strand at 4051.417 kb on - strand at 4051.461 kb on + strand at 4051.469 kb on + strand at 4051.531 kb on - strand at 4051.557 kb on + strand at 4051.652 kb on + strand, within HP15_3825 at 4051.740 kb on - strand, within HP15_3825 at 4051.872 kb on - strand, within HP15_3825 at 4051.966 kb on - strand, within HP15_3825 at 4052.018 kb on - strand, within HP15_3825 at 4052.045 kb on + strand, within HP15_3825 at 4052.160 kb on - strand, within HP15_3825 at 4052.160 kb on - strand, within HP15_3825 at 4052.167 kb on - strand, within HP15_3825 at 4052.213 kb on + strand, within HP15_3825
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 4,049,294 - +0.0 4,049,299 - +0.3 4,049,299 - -0.7 4,049,385 + +0.6 4,049,385 + +0.7 4,049,412 - +0.0 4,049,443 + +0.2 4,049,451 - -0.2 4,049,525 - +0.8 4,049,784 - HP15_3823 0.27 -0.5 4,049,807 + HP15_3823 0.31 -0.2 4,049,833 - HP15_3823 0.36 -0.1 4,049,895 - HP15_3823 0.46 -0.2 4,049,914 + HP15_3823 0.50 +0.8 4,049,986 + HP15_3823 0.62 -0.3 4,050,035 + HP15_3823 0.71 +0.2 4,050,038 - HP15_3823 0.72 -0.5 4,050,042 + HP15_3823 0.72 +0.8 4,050,077 - HP15_3823 0.78 +0.3 4,050,113 + HP15_3823 0.85 -0.5 4,050,171 + +0.3 4,050,171 + +0.0 4,050,404 - HP15_3824 0.15 -0.6 4,050,470 + HP15_3824 0.21 +0.4 4,050,478 - HP15_3824 0.22 -0.6 4,050,503 + HP15_3824 0.24 -3.0 4,050,511 - HP15_3824 0.25 -0.6 4,050,661 - HP15_3824 0.39 -0.1 4,050,693 + HP15_3824 0.42 +0.4 4,050,693 + HP15_3824 0.42 -1.3 4,050,763 - HP15_3824 0.49 -0.6 4,050,775 - HP15_3824 0.50 +1.2 4,050,846 - HP15_3824 0.57 -1.2 4,050,980 + HP15_3824 0.70 -0.6 4,050,998 - HP15_3824 0.71 -2.1 4,051,090 + HP15_3824 0.80 +0.5 4,051,109 + HP15_3824 0.82 +0.1 4,051,278 + -0.0 4,051,328 + +1.6 4,051,328 + -0.1 4,051,328 + -0.3 4,051,328 + +0.2 4,051,328 + -0.2 4,051,336 - +0.8 4,051,336 - +0.1 4,051,336 - -0.6 4,051,336 - +0.3 4,051,336 - -1.3 4,051,396 - +0.4 4,051,417 - +0.0 4,051,461 + -0.2 4,051,469 + +0.1 4,051,531 - -0.4 4,051,557 + -0.8 4,051,652 + HP15_3825 0.16 -0.3 4,051,740 - HP15_3825 0.22 +0.5 4,051,872 - HP15_3825 0.31 -0.1 4,051,966 - HP15_3825 0.37 +0.3 4,052,018 - HP15_3825 0.41 +0.2 4,052,045 + HP15_3825 0.42 -0.4 4,052,160 - HP15_3825 0.50 +0.8 4,052,160 - HP15_3825 0.50 +0.1 4,052,167 - HP15_3825 0.51 -0.5 4,052,213 + HP15_3825 0.54 -0.6
Or see this region's nucleotide sequence