Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3713 and HP15_3714 overlap by 4 nucleotides HP15_3714 and HP15_3715 are separated by 155 nucleotides HP15_3715 and HP15_3716 are separated by 18 nucleotides
HP15_3713: HP15_3713 - exodeoxyribonuclease V, beta subunit, at 3,923,533 to 3,927,237
_3713
HP15_3714: HP15_3714 - exodeoxyribonuclease V, gamma subunit, at 3,927,234 to 3,930,860
_3714
HP15_3715: HP15_3715 - short-chain dehydrogenase/reductase SDR, at 3,931,016 to 3,931,801
_3715
HP15_3716: HP15_3716 - conserved hypothetical protein, at 3,931,820 to 3,932,110
_3716
Position (kb)
3927
3928
3929
3930
3931 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3927.303 kb on - strand at 3928.082 kb on + strand, within HP15_3714 at 3929.055 kb on - strand, within HP15_3714 at 3929.131 kb on + strand, within HP15_3714 at 3929.961 kb on - strand, within HP15_3714 at 3929.961 kb on - strand, within HP15_3714 at 3930.349 kb on + strand, within HP15_3714 at 3930.811 kb on + strand at 3930.812 kb on - strand at 3930.878 kb on + strand at 3930.883 kb on + strand at 3930.883 kb on + strand at 3930.922 kb on + strand at 3930.932 kb on + strand at 3931.065 kb on + strand at 3931.067 kb on - strand at 3931.087 kb on - strand at 3931.087 kb on - strand at 3931.087 kb on - strand at 3931.091 kb on + strand at 3931.099 kb on - strand, within HP15_3715 at 3931.137 kb on + strand, within HP15_3715 at 3931.187 kb on - strand, within HP15_3715 at 3931.193 kb on - strand, within HP15_3715 at 3931.270 kb on - strand, within HP15_3715 at 3931.272 kb on + strand, within HP15_3715 at 3931.277 kb on + strand, within HP15_3715 at 3931.285 kb on - strand, within HP15_3715 at 3931.308 kb on + strand, within HP15_3715 at 3931.308 kb on + strand, within HP15_3715 at 3931.316 kb on - strand, within HP15_3715 at 3931.341 kb on - strand, within HP15_3715 at 3931.441 kb on + strand, within HP15_3715 at 3931.503 kb on - strand, within HP15_3715 at 3931.525 kb on + strand, within HP15_3715 at 3931.534 kb on - strand, within HP15_3715 at 3931.573 kb on + strand, within HP15_3715 at 3931.587 kb on - strand, within HP15_3715 at 3931.625 kb on + strand, within HP15_3715 at 3931.688 kb on - strand, within HP15_3715 at 3931.748 kb on + strand at 3931.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 3,927,303 - +0.3 3,928,082 + HP15_3714 0.23 -1.4 3,929,055 - HP15_3714 0.50 -0.4 3,929,131 + HP15_3714 0.52 -1.0 3,929,961 - HP15_3714 0.75 +0.5 3,929,961 - HP15_3714 0.75 -1.0 3,930,349 + HP15_3714 0.86 -1.5 3,930,811 + -0.4 3,930,812 - -0.2 3,930,878 + -0.0 3,930,883 + -0.9 3,930,883 + +0.0 3,930,922 + +0.6 3,930,932 + +2.7 3,931,065 + -0.5 3,931,067 - +0.2 3,931,087 - +0.2 3,931,087 - -0.7 3,931,087 - -0.5 3,931,091 + +0.5 3,931,099 - HP15_3715 0.11 +0.8 3,931,137 + HP15_3715 0.15 -0.4 3,931,187 - HP15_3715 0.22 -0.2 3,931,193 - HP15_3715 0.23 -0.1 3,931,270 - HP15_3715 0.32 -0.7 3,931,272 + HP15_3715 0.33 +0.4 3,931,277 + HP15_3715 0.33 +0.2 3,931,285 - HP15_3715 0.34 -0.2 3,931,308 + HP15_3715 0.37 +1.3 3,931,308 + HP15_3715 0.37 +0.2 3,931,316 - HP15_3715 0.38 -0.4 3,931,341 - HP15_3715 0.41 -0.7 3,931,441 + HP15_3715 0.54 -0.1 3,931,503 - HP15_3715 0.62 +1.2 3,931,525 + HP15_3715 0.65 +0.2 3,931,534 - HP15_3715 0.66 -0.0 3,931,573 + HP15_3715 0.71 +0.7 3,931,587 - HP15_3715 0.73 -1.8 3,931,625 + HP15_3715 0.77 -0.3 3,931,688 - HP15_3715 0.85 -0.2 3,931,748 + +0.1 3,931,809 - +0.6
Or see this region's nucleotide sequence