Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3519 and HP15_3520 are separated by 11 nucleotides HP15_3520 and HP15_3521 are separated by 73 nucleotides HP15_3521 and HP15_3522 overlap by 8 nucleotides HP15_3522 and HP15_3523 overlap by 14 nucleotides
HP15_3519: HP15_3519 - CheB methylesterase, at 3,708,128 to 3,709,180
_3519
HP15_3520: HP15_3520 - protein containing CheW-like domain, at 3,709,192 to 3,709,653
_3520
HP15_3521: HP15_3521 - zinc uptake regulation protein, at 3,709,727 to 3,710,218
_3521
HP15_3522: HP15_3522 - zinc import ATP-binding protein znuC, at 3,710,211 to 3,710,978
_3522
HP15_3523: HP15_3523 - zinc ABC transporter, permease protein, at 3,710,965 to 3,711,777
_3523
Position (kb)
3709
3710
3711 Strain fitness (log2 ratio)
-2
-1
0
1 at 3708.788 kb on - strand, within HP15_3519 at 3708.873 kb on + strand, within HP15_3519 at 3708.918 kb on + strand, within HP15_3519 at 3708.926 kb on - strand, within HP15_3519 at 3709.022 kb on - strand, within HP15_3519 at 3709.203 kb on + strand at 3709.257 kb on + strand, within HP15_3520 at 3709.499 kb on + strand, within HP15_3520 at 3709.932 kb on - strand, within HP15_3521 at 3710.097 kb on + strand, within HP15_3521 at 3710.097 kb on + strand, within HP15_3521 at 3710.105 kb on - strand, within HP15_3521 at 3710.105 kb on - strand, within HP15_3521 at 3710.109 kb on + strand, within HP15_3521 at 3710.759 kb on + strand, within HP15_3522 at 3710.914 kb on + strand at 3710.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 3,708,788 - HP15_3519 0.63 +0.4 3,708,873 + HP15_3519 0.71 -0.4 3,708,918 + HP15_3519 0.75 +0.6 3,708,926 - HP15_3519 0.76 +0.7 3,709,022 - HP15_3519 0.85 +0.3 3,709,203 + +0.0 3,709,257 + HP15_3520 0.14 +0.1 3,709,499 + HP15_3520 0.66 -0.9 3,709,932 - HP15_3521 0.42 +0.1 3,710,097 + HP15_3521 0.75 -1.3 3,710,097 + HP15_3521 0.75 +0.2 3,710,105 - HP15_3521 0.77 -2.3 3,710,105 - HP15_3521 0.77 -0.2 3,710,109 + HP15_3521 0.78 -1.0 3,710,759 + HP15_3522 0.71 -1.1 3,710,914 + -0.7 3,710,922 - -2.2
Or see this region's nucleotide sequence