Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3440 and HP15_3441 are separated by 322 nucleotides HP15_3441 and HP15_3442 are separated by 82 nucleotides HP15_3442 and HP15_3443 are separated by 23 nucleotides HP15_3443 and HP15_3444 are separated by 95 nucleotides
HP15_3440: HP15_3440 - integral membrane protein, at 3,619,262 to 3,620,710
_3440
HP15_3441: HP15_3441 - OmpA/MotB domain protein, at 3,621,033 to 3,621,473
_3441
HP15_3442: HP15_3442 - conserved hypothetical protein, membrane, at 3,621,556 to 3,621,936
_3442
HP15_3443: HP15_3443 - transglycosylase-associated-like protein, at 3,621,960 to 3,622,202
_3443
HP15_3444: HP15_3444 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 3,622,298 to 3,622,996
_3444
Position (kb)
3621
3622 Strain fitness (log2 ratio)
-1
0
1
2 at 3620.056 kb on - strand, within HP15_3440 at 3620.056 kb on - strand, within HP15_3440 at 3620.104 kb on - strand, within HP15_3440 at 3620.104 kb on - strand, within HP15_3440 at 3620.141 kb on + strand, within HP15_3440 at 3620.141 kb on + strand, within HP15_3440 at 3620.191 kb on - strand, within HP15_3440 at 3620.193 kb on + strand, within HP15_3440 at 3620.239 kb on + strand, within HP15_3440 at 3620.334 kb on - strand, within HP15_3440 at 3620.472 kb on - strand, within HP15_3440 at 3620.491 kb on - strand, within HP15_3440 at 3620.503 kb on + strand, within HP15_3440 at 3620.570 kb on - strand at 3620.572 kb on + strand at 3620.572 kb on + strand at 3620.653 kb on + strand at 3620.653 kb on + strand at 3620.655 kb on - strand at 3620.655 kb on - strand at 3620.655 kb on - strand at 3620.665 kb on - strand at 3620.795 kb on - strand at 3620.927 kb on + strand at 3620.976 kb on + strand at 3621.063 kb on - strand at 3621.389 kb on + strand, within HP15_3441 at 3621.395 kb on + strand, within HP15_3441 at 3621.510 kb on + strand at 3621.627 kb on - strand, within HP15_3442 at 3621.682 kb on + strand, within HP15_3442 at 3621.746 kb on + strand, within HP15_3442 at 3621.754 kb on - strand, within HP15_3442 at 3621.766 kb on - strand, within HP15_3442 at 3621.799 kb on - strand, within HP15_3442 at 3621.819 kb on - strand, within HP15_3442 at 3621.819 kb on - strand, within HP15_3442 at 3622.222 kb on - strand at 3622.280 kb on + strand at 3622.280 kb on - strand at 3622.280 kb on - strand at 3622.280 kb on - strand at 3622.366 kb on - strand at 3622.388 kb on - strand, within HP15_3444 at 3622.423 kb on + strand, within HP15_3444
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 3,620,056 - HP15_3440 0.55 +0.2 3,620,056 - HP15_3440 0.55 +0.5 3,620,104 - HP15_3440 0.58 +2.2 3,620,104 - HP15_3440 0.58 +0.5 3,620,141 + HP15_3440 0.61 +0.0 3,620,141 + HP15_3440 0.61 +1.2 3,620,191 - HP15_3440 0.64 -0.3 3,620,193 + HP15_3440 0.64 +0.5 3,620,239 + HP15_3440 0.67 +1.2 3,620,334 - HP15_3440 0.74 -0.1 3,620,472 - HP15_3440 0.84 -0.0 3,620,491 - HP15_3440 0.85 -0.5 3,620,503 + HP15_3440 0.86 -0.2 3,620,570 - -0.1 3,620,572 + +0.1 3,620,572 + +0.5 3,620,653 + +0.1 3,620,653 + -0.0 3,620,655 - +0.6 3,620,655 - -0.1 3,620,655 - +0.6 3,620,665 - +0.6 3,620,795 - +0.5 3,620,927 + -0.6 3,620,976 + +1.3 3,621,063 - -0.5 3,621,389 + HP15_3441 0.81 -0.2 3,621,395 + HP15_3441 0.82 -1.1 3,621,510 + +0.4 3,621,627 - HP15_3442 0.19 -0.2 3,621,682 + HP15_3442 0.33 +0.7 3,621,746 + HP15_3442 0.50 -0.1 3,621,754 - HP15_3442 0.52 -0.3 3,621,766 - HP15_3442 0.55 +0.2 3,621,799 - HP15_3442 0.64 +0.8 3,621,819 - HP15_3442 0.69 -0.0 3,621,819 - HP15_3442 0.69 +0.1 3,622,222 - -0.0 3,622,280 + +0.0 3,622,280 - +1.0 3,622,280 - +0.5 3,622,280 - +0.3 3,622,366 - -0.8 3,622,388 - HP15_3444 0.13 +0.7 3,622,423 + HP15_3444 0.18 +0.5
Or see this region's nucleotide sequence