Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3274 and HP15_3275 are separated by 66 nucleotides HP15_3275 and HP15_3276 are separated by 25 nucleotides HP15_3276 and HP15_3277 are separated by 43 nucleotides HP15_3277 and HP15_3278 overlap by 8 nucleotides HP15_3278 and HP15_3279 overlap by 4 nucleotides
HP15_3274: HP15_3274 - thiazole synthase, at 3,432,815 to 3,433,624
_3274
HP15_3275: HP15_3275 - ThiS, thiamine-biosynthesis, at 3,433,691 to 3,433,891
_3275
HP15_3276: HP15_3276 - membrane protein containing DUF423, at 3,433,917 to 3,434,276
_3276
HP15_3277: HP15_3277 - diadenosine tetraphosphatase, at 3,434,320 to 3,435,120
_3277
HP15_3278: HP15_3278 - dimethyladenosine transferase, at 3,435,113 to 3,435,943
_3278
HP15_3279: HP15_3279 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,435,940 to 3,436,950
_3279
Position (kb)
3434
3435
3436 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3433.620 kb on + strand at 3433.628 kb on - strand at 3433.628 kb on - strand at 3433.661 kb on - strand at 3433.683 kb on - strand at 3433.687 kb on + strand at 3433.689 kb on - strand at 3433.950 kb on - strand at 3434.277 kb on - strand at 3434.577 kb on - strand, within HP15_3277 at 3434.727 kb on - strand, within HP15_3277 at 3435.058 kb on + strand at 3435.245 kb on - strand, within HP15_3278 at 3435.266 kb on + strand, within HP15_3278 at 3435.490 kb on - strand, within HP15_3278 at 3435.569 kb on - strand, within HP15_3278 at 3435.811 kb on - strand, within HP15_3278 at 3435.811 kb on - strand, within HP15_3278 at 3435.811 kb on - strand, within HP15_3278 at 3435.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 3,433,620 + -0.4 3,433,628 - -1.0 3,433,628 - +1.0 3,433,661 - +0.0 3,433,683 - -0.6 3,433,687 + -0.2 3,433,689 - -0.2 3,433,950 - -0.6 3,434,277 - +0.0 3,434,577 - HP15_3277 0.32 -1.9 3,434,727 - HP15_3277 0.51 -1.8 3,435,058 + -3.5 3,435,245 - HP15_3278 0.16 +0.0 3,435,266 + HP15_3278 0.18 +0.3 3,435,490 - HP15_3278 0.45 +1.3 3,435,569 - HP15_3278 0.55 +0.0 3,435,811 - HP15_3278 0.84 +0.1 3,435,811 - HP15_3278 0.84 +0.6 3,435,811 - HP15_3278 0.84 -0.1 3,435,868 + -0.8
Or see this region's nucleotide sequence