Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3116 and HP15_3117 overlap by 4 nucleotides HP15_3117 and HP15_3118 are separated by 47 nucleotides HP15_3118 and HP15_3119 are separated by 48 nucleotides
HP15_3116: HP15_3116 - response regulator receiver modulated CheW protein, at 3,266,309 to 3,267,196
_3116
HP15_3117: HP15_3117 - conserved hypothetical protein, at 3,267,193 to 3,268,056
_3117
HP15_3118: HP15_3118 - acetolactate synthase, large subunit, biosynthetic type, at 3,268,104 to 3,269,774
_3118
HP15_3119: HP15_3119 - conserved hypothetical protein, at 3,269,823 to 3,271,871
_3119
Position (kb)
3268
3269
3270 Strain fitness (log2 ratio)
-1
0
1
2 at 3267.120 kb on - strand at 3267.157 kb on + strand at 3267.180 kb on - strand at 3267.200 kb on - strand at 3267.205 kb on - strand at 3267.235 kb on + strand at 3267.243 kb on - strand at 3267.405 kb on + strand, within HP15_3117 at 3267.599 kb on - strand, within HP15_3117 at 3267.680 kb on + strand, within HP15_3117 at 3267.680 kb on + strand, within HP15_3117 at 3267.854 kb on + strand, within HP15_3117 at 3267.911 kb on - strand, within HP15_3117 at 3267.916 kb on - strand, within HP15_3117 at 3267.980 kb on + strand at 3267.980 kb on + strand at 3267.988 kb on - strand at 3268.102 kb on + strand at 3268.102 kb on + strand at 3268.110 kb on - strand at 3268.173 kb on + strand at 3268.192 kb on - strand at 3268.192 kb on - strand at 3268.195 kb on + strand at 3268.385 kb on + strand, within HP15_3118 at 3268.439 kb on - strand, within HP15_3118 at 3268.495 kb on - strand, within HP15_3118 at 3268.517 kb on - strand, within HP15_3118 at 3268.530 kb on + strand, within HP15_3118 at 3268.538 kb on - strand, within HP15_3118 at 3268.665 kb on + strand, within HP15_3118 at 3268.701 kb on - strand, within HP15_3118 at 3268.787 kb on - strand, within HP15_3118 at 3268.793 kb on + strand, within HP15_3118 at 3268.799 kb on - strand, within HP15_3118 at 3268.972 kb on - strand, within HP15_3118 at 3268.991 kb on - strand, within HP15_3118 at 3269.064 kb on - strand, within HP15_3118 at 3269.309 kb on + strand, within HP15_3118 at 3269.317 kb on - strand, within HP15_3118 at 3269.317 kb on - strand, within HP15_3118 at 3269.322 kb on + strand, within HP15_3118 at 3269.322 kb on + strand, within HP15_3118 at 3269.329 kb on - strand, within HP15_3118 at 3269.353 kb on + strand, within HP15_3118 at 3269.360 kb on - strand, within HP15_3118 at 3269.451 kb on - strand, within HP15_3118 at 3269.487 kb on + strand, within HP15_3118 at 3269.585 kb on - strand, within HP15_3118 at 3269.617 kb on - strand at 3269.769 kb on + strand at 3269.782 kb on + strand at 3269.923 kb on - strand at 3269.949 kb on + strand at 3269.983 kb on + strand at 3269.991 kb on - strand at 3270.001 kb on - strand at 3270.066 kb on + strand, within HP15_3119 at 3270.069 kb on - strand, within HP15_3119 at 3270.074 kb on - strand, within HP15_3119 at 3270.242 kb on + strand, within HP15_3119 at 3270.444 kb on - strand, within HP15_3119 at 3270.554 kb on - strand, within HP15_3119 at 3270.554 kb on - strand, within HP15_3119 at 3270.564 kb on - strand, within HP15_3119 at 3270.642 kb on + strand, within HP15_3119 at 3270.661 kb on + strand, within HP15_3119 at 3270.663 kb on - strand, within HP15_3119 at 3270.721 kb on + strand, within HP15_3119 at 3270.729 kb on - strand, within HP15_3119 at 3270.739 kb on - strand, within HP15_3119
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 3,267,120 - +0.6 3,267,157 + -0.7 3,267,180 - +0.2 3,267,200 - -0.2 3,267,205 - -0.4 3,267,235 + -0.2 3,267,243 - +0.7 3,267,405 + HP15_3117 0.25 -0.2 3,267,599 - HP15_3117 0.47 -0.5 3,267,680 + HP15_3117 0.56 -0.1 3,267,680 + HP15_3117 0.56 +0.5 3,267,854 + HP15_3117 0.77 -0.1 3,267,911 - HP15_3117 0.83 +0.8 3,267,916 - HP15_3117 0.84 -0.5 3,267,980 + -0.2 3,267,980 + +0.2 3,267,988 - -0.2 3,268,102 + +0.2 3,268,102 + -0.9 3,268,110 - -0.2 3,268,173 + -0.7 3,268,192 - -0.1 3,268,192 - -0.7 3,268,195 + -0.4 3,268,385 + HP15_3118 0.17 +0.1 3,268,439 - HP15_3118 0.20 +0.7 3,268,495 - HP15_3118 0.23 -0.4 3,268,517 - HP15_3118 0.25 +0.4 3,268,530 + HP15_3118 0.25 -0.3 3,268,538 - HP15_3118 0.26 -0.4 3,268,665 + HP15_3118 0.34 -0.0 3,268,701 - HP15_3118 0.36 -0.2 3,268,787 - HP15_3118 0.41 +0.9 3,268,793 + HP15_3118 0.41 -0.3 3,268,799 - HP15_3118 0.42 -0.4 3,268,972 - HP15_3118 0.52 +0.6 3,268,991 - HP15_3118 0.53 +0.7 3,269,064 - HP15_3118 0.57 -1.3 3,269,309 + HP15_3118 0.72 +0.4 3,269,317 - HP15_3118 0.73 +0.6 3,269,317 - HP15_3118 0.73 +0.1 3,269,322 + HP15_3118 0.73 +1.3 3,269,322 + HP15_3118 0.73 +0.7 3,269,329 - HP15_3118 0.73 -0.8 3,269,353 + HP15_3118 0.75 -0.2 3,269,360 - HP15_3118 0.75 +0.6 3,269,451 - HP15_3118 0.81 -0.5 3,269,487 + HP15_3118 0.83 -0.2 3,269,585 - HP15_3118 0.89 -1.3 3,269,617 - -0.8 3,269,769 + +0.7 3,269,782 + -1.0 3,269,923 - +0.9 3,269,949 + +0.4 3,269,983 + +0.2 3,269,991 - -0.9 3,270,001 - -0.7 3,270,066 + HP15_3119 0.12 -0.8 3,270,069 - HP15_3119 0.12 -0.1 3,270,074 - HP15_3119 0.12 +0.7 3,270,242 + HP15_3119 0.20 -0.0 3,270,444 - HP15_3119 0.30 +0.3 3,270,554 - HP15_3119 0.36 +0.6 3,270,554 - HP15_3119 0.36 -0.3 3,270,564 - HP15_3119 0.36 +0.1 3,270,642 + HP15_3119 0.40 +1.1 3,270,661 + HP15_3119 0.41 +0.8 3,270,663 - HP15_3119 0.41 +0.3 3,270,721 + HP15_3119 0.44 -0.1 3,270,729 - HP15_3119 0.44 +2.4 3,270,739 - HP15_3119 0.45 -0.7
Or see this region's nucleotide sequence