Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2916 and HP15_2917 are separated by 12 nucleotides HP15_2917 and HP15_2918 are separated by 250 nucleotides
HP15_2916: HP15_2916 - adenosylhomocysteinase, at 3,055,331 to 3,056,725
_2916
HP15_2917: HP15_2917 - 5,10-methylenetetrahydrofolate reductase-like protein, at 3,056,738 to 3,057,607
_2917
HP15_2918: HP15_2918 - glutamine ABC transporter, periplasmic glutamine-binding protein, at 3,057,858 to 3,058,616
_2918
Position (kb)
3056
3057
3058 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3056.796 kb on - strand at 3056.811 kb on - strand at 3056.892 kb on + strand, within HP15_2917 at 3057.055 kb on + strand, within HP15_2917 at 3057.070 kb on - strand, within HP15_2917 at 3057.080 kb on + strand, within HP15_2917 at 3057.143 kb on - strand, within HP15_2917 at 3057.181 kb on - strand, within HP15_2917 at 3057.220 kb on + strand, within HP15_2917 at 3057.221 kb on + strand, within HP15_2917 at 3057.229 kb on - strand, within HP15_2917 at 3057.229 kb on - strand, within HP15_2917 at 3057.229 kb on - strand, within HP15_2917 at 3057.229 kb on - strand, within HP15_2917 at 3057.229 kb on - strand, within HP15_2917 at 3057.229 kb on - strand, within HP15_2917 at 3057.248 kb on + strand, within HP15_2917 at 3057.343 kb on - strand, within HP15_2917 at 3057.387 kb on + strand, within HP15_2917 at 3057.443 kb on + strand, within HP15_2917 at 3057.493 kb on + strand, within HP15_2917 at 3057.521 kb on + strand at 3057.539 kb on - strand at 3057.539 kb on - strand at 3057.654 kb on - strand at 3057.782 kb on - strand at 3057.886 kb on + strand at 3057.892 kb on + strand at 3058.069 kb on - strand, within HP15_2918 at 3058.149 kb on - strand, within HP15_2918 at 3058.287 kb on + strand, within HP15_2918 at 3058.287 kb on + strand, within HP15_2918 at 3058.327 kb on + strand, within HP15_2918 at 3058.341 kb on - strand, within HP15_2918 at 3058.348 kb on + strand, within HP15_2918 at 3058.348 kb on + strand, within HP15_2918 at 3058.356 kb on - strand, within HP15_2918 at 3058.356 kb on - strand, within HP15_2918 at 3058.392 kb on + strand, within HP15_2918 at 3058.400 kb on - strand, within HP15_2918 at 3058.402 kb on - strand, within HP15_2918 at 3058.430 kb on + strand, within HP15_2918 at 3058.451 kb on - strand, within HP15_2918 at 3058.484 kb on - strand, within HP15_2918 at 3058.512 kb on - strand, within HP15_2918 at 3058.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 3,056,796 - -3.2 3,056,811 - -1.1 3,056,892 + HP15_2917 0.18 -3.1 3,057,055 + HP15_2917 0.36 -3.0 3,057,070 - HP15_2917 0.38 -1.7 3,057,080 + HP15_2917 0.39 -1.5 3,057,143 - HP15_2917 0.47 -2.9 3,057,181 - HP15_2917 0.51 -2.3 3,057,220 + HP15_2917 0.55 -0.5 3,057,221 + HP15_2917 0.56 -3.6 3,057,229 - HP15_2917 0.56 -2.5 3,057,229 - HP15_2917 0.56 -4.1 3,057,229 - HP15_2917 0.56 -2.3 3,057,229 - HP15_2917 0.56 -2.1 3,057,229 - HP15_2917 0.56 -3.5 3,057,229 - HP15_2917 0.56 -1.7 3,057,248 + HP15_2917 0.59 -1.1 3,057,343 - HP15_2917 0.70 -1.2 3,057,387 + HP15_2917 0.75 -1.8 3,057,443 + HP15_2917 0.81 -1.4 3,057,493 + HP15_2917 0.87 -1.6 3,057,521 + -3.7 3,057,539 - -2.5 3,057,539 - -1.5 3,057,654 - +0.1 3,057,782 - -1.3 3,057,886 + -0.2 3,057,892 + -0.4 3,058,069 - HP15_2918 0.28 -0.1 3,058,149 - HP15_2918 0.38 -0.5 3,058,287 + HP15_2918 0.57 +0.7 3,058,287 + HP15_2918 0.57 +1.0 3,058,327 + HP15_2918 0.62 +0.1 3,058,341 - HP15_2918 0.64 +0.3 3,058,348 + HP15_2918 0.65 -0.3 3,058,348 + HP15_2918 0.65 +0.6 3,058,356 - HP15_2918 0.66 -0.4 3,058,356 - HP15_2918 0.66 -0.7 3,058,392 + HP15_2918 0.70 +0.7 3,058,400 - HP15_2918 0.71 -0.1 3,058,402 - HP15_2918 0.72 +1.0 3,058,430 + HP15_2918 0.75 +0.7 3,058,451 - HP15_2918 0.78 +0.3 3,058,484 - HP15_2918 0.82 +0.1 3,058,512 - HP15_2918 0.86 -0.9 3,058,553 + -0.3
Or see this region's nucleotide sequence