Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2776 and HP15_2777 are separated by 110 nucleotides HP15_2777 and HP15_2778 are separated by 24 nucleotides HP15_2778 and HP15_2779 are separated by 40 nucleotides
HP15_2776: HP15_2776 - membrane protein containing DUF6, transmembrane, at 2,928,482 to 2,929,384
_2776
HP15_2777: HP15_2777 - penicillin-binding protein 1B, at 2,929,495 to 2,929,707
_2777
HP15_2778: HP15_2778 - transcriptional regulator, at 2,929,732 to 2,930,400
_2778
HP15_2779: HP15_2779 - GTP cyclohydrolase II, at 2,930,441 to 2,930,920
_2779
Position (kb)
2929
2930 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2928.508 kb on - strand at 2928.567 kb on + strand at 2928.567 kb on + strand at 2928.631 kb on - strand, within HP15_2776 at 2928.636 kb on - strand, within HP15_2776 at 2928.679 kb on + strand, within HP15_2776 at 2928.759 kb on + strand, within HP15_2776 at 2928.759 kb on + strand, within HP15_2776 at 2928.813 kb on + strand, within HP15_2776 at 2928.813 kb on + strand, within HP15_2776 at 2928.816 kb on + strand, within HP15_2776 at 2928.820 kb on - strand, within HP15_2776 at 2928.820 kb on - strand, within HP15_2776 at 2928.831 kb on + strand, within HP15_2776 at 2928.999 kb on - strand, within HP15_2776 at 2929.058 kb on - strand, within HP15_2776 at 2929.127 kb on - strand, within HP15_2776 at 2929.152 kb on + strand, within HP15_2776 at 2929.232 kb on - strand, within HP15_2776 at 2929.422 kb on - strand at 2929.422 kb on - strand at 2929.422 kb on - strand at 2929.422 kb on - strand at 2929.477 kb on + strand at 2929.477 kb on + strand at 2929.477 kb on + strand at 2929.485 kb on - strand at 2929.485 kb on - strand at 2929.651 kb on - strand, within HP15_2777 at 2929.743 kb on - strand at 2929.799 kb on + strand, within HP15_2778 at 2929.871 kb on - strand, within HP15_2778 at 2929.886 kb on - strand, within HP15_2778 at 2929.903 kb on - strand, within HP15_2778 at 2930.088 kb on - strand, within HP15_2778 at 2930.115 kb on - strand, within HP15_2778 at 2930.202 kb on + strand, within HP15_2778 at 2930.208 kb on + strand, within HP15_2778 at 2930.210 kb on - strand, within HP15_2778 at 2930.265 kb on + strand, within HP15_2778 at 2930.267 kb on - strand, within HP15_2778 at 2930.305 kb on + strand, within HP15_2778 at 2930.402 kb on - strand at 2930.413 kb on + strand at 2930.494 kb on - strand, within HP15_2779 at 2930.588 kb on + strand, within HP15_2779 at 2930.603 kb on - strand, within HP15_2779 at 2930.652 kb on + strand, within HP15_2779 at 2930.662 kb on + strand, within HP15_2779 at 2930.687 kb on + strand, within HP15_2779
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,928,508 - -0.1 2,928,567 + +1.8 2,928,567 + +0.8 2,928,631 - HP15_2776 0.17 +0.2 2,928,636 - HP15_2776 0.17 +0.9 2,928,679 + HP15_2776 0.22 +1.3 2,928,759 + HP15_2776 0.31 +0.1 2,928,759 + HP15_2776 0.31 -0.4 2,928,813 + HP15_2776 0.37 +0.1 2,928,813 + HP15_2776 0.37 +0.8 2,928,816 + HP15_2776 0.37 -0.0 2,928,820 - HP15_2776 0.37 +0.3 2,928,820 - HP15_2776 0.37 +1.5 2,928,831 + HP15_2776 0.39 -0.3 2,928,999 - HP15_2776 0.57 +0.0 2,929,058 - HP15_2776 0.64 -1.8 2,929,127 - HP15_2776 0.71 +0.6 2,929,152 + HP15_2776 0.74 +1.7 2,929,232 - HP15_2776 0.83 -0.0 2,929,422 - -0.4 2,929,422 - +0.4 2,929,422 - +0.4 2,929,422 - -0.0 2,929,477 + +0.3 2,929,477 + +1.8 2,929,477 + +0.8 2,929,485 - +0.3 2,929,485 - -0.8 2,929,651 - HP15_2777 0.73 -0.4 2,929,743 - +1.1 2,929,799 + HP15_2778 0.10 +0.8 2,929,871 - HP15_2778 0.21 +1.0 2,929,886 - HP15_2778 0.23 -0.3 2,929,903 - HP15_2778 0.26 -0.2 2,930,088 - HP15_2778 0.53 +0.1 2,930,115 - HP15_2778 0.57 +0.6 2,930,202 + HP15_2778 0.70 -0.7 2,930,208 + HP15_2778 0.71 +1.7 2,930,210 - HP15_2778 0.71 -0.1 2,930,265 + HP15_2778 0.80 -0.2 2,930,267 - HP15_2778 0.80 -0.6 2,930,305 + HP15_2778 0.86 -0.7 2,930,402 - +0.2 2,930,413 + +0.3 2,930,494 - HP15_2779 0.11 -0.3 2,930,588 + HP15_2779 0.31 -0.3 2,930,603 - HP15_2779 0.34 +0.9 2,930,652 + HP15_2779 0.44 -0.8 2,930,662 + HP15_2779 0.46 -0.0 2,930,687 + HP15_2779 0.51 +0.3
Or see this region's nucleotide sequence