Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2684 and HP15_2685 are separated by 31 nucleotides HP15_2685 and HP15_2686 overlap by 4 nucleotides HP15_2686 and HP15_2687 overlap by 11 nucleotides HP15_2687 and HP15_2688 are separated by 38 nucleotides
HP15_2684: HP15_2684 - TRAP-type C4-dicarboxylate transport system, large permease component, at 2,831,671 to 2,832,978
_2684
HP15_2685: HP15_2685 - UPF0271 protein, LamB/YcsF family, at 2,833,010 to 2,833,744
_2685
HP15_2686: HP15_2686 - allophanate hydrolase subunit 1, at 2,833,741 to 2,834,433
_2686
HP15_2687: HP15_2687 - urea amidolyase related protein, at 2,834,423 to 2,835,361
_2687
HP15_2688: HP15_2688 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 2,835,400 to 2,839,029
_2688
Position (kb)
2833
2834
2835 Strain fitness (log2 ratio)
-2
-1
0
1 at 2832.791 kb on + strand, within HP15_2684 at 2832.809 kb on - strand, within HP15_2684 at 2832.826 kb on - strand, within HP15_2684 at 2832.858 kb on - strand at 2833.008 kb on - strand at 2833.141 kb on - strand, within HP15_2685 at 2833.197 kb on + strand, within HP15_2685 at 2833.205 kb on - strand, within HP15_2685 at 2833.249 kb on + strand, within HP15_2685 at 2833.489 kb on + strand, within HP15_2685 at 2833.584 kb on + strand, within HP15_2685 at 2833.652 kb on - strand, within HP15_2685 at 2833.889 kb on + strand, within HP15_2686 at 2833.889 kb on + strand, within HP15_2686 at 2833.894 kb on + strand, within HP15_2686 at 2833.940 kb on + strand, within HP15_2686 at 2834.049 kb on - strand, within HP15_2686 at 2834.124 kb on + strand, within HP15_2686 at 2834.176 kb on - strand, within HP15_2686 at 2834.355 kb on + strand, within HP15_2686 at 2834.526 kb on + strand, within HP15_2687 at 2834.601 kb on + strand, within HP15_2687 at 2834.713 kb on + strand, within HP15_2687 at 2834.778 kb on + strand, within HP15_2687 at 2834.877 kb on - strand, within HP15_2687 at 2835.309 kb on + strand at 2835.351 kb on + strand at 2835.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,832,791 + HP15_2684 0.86 -0.5 2,832,809 - HP15_2684 0.87 -0.1 2,832,826 - HP15_2684 0.88 +0.9 2,832,858 - +0.5 2,833,008 - -0.3 2,833,141 - HP15_2685 0.18 -1.9 2,833,197 + HP15_2685 0.25 -0.7 2,833,205 - HP15_2685 0.27 -1.3 2,833,249 + HP15_2685 0.33 -0.2 2,833,489 + HP15_2685 0.65 +0.3 2,833,584 + HP15_2685 0.78 +0.2 2,833,652 - HP15_2685 0.87 -0.2 2,833,889 + HP15_2686 0.21 -0.6 2,833,889 + HP15_2686 0.21 -1.3 2,833,894 + HP15_2686 0.22 -0.6 2,833,940 + HP15_2686 0.29 -0.2 2,834,049 - HP15_2686 0.44 +1.3 2,834,124 + HP15_2686 0.55 -0.6 2,834,176 - HP15_2686 0.63 +0.1 2,834,355 + HP15_2686 0.89 -1.5 2,834,526 + HP15_2687 0.11 -1.7 2,834,601 + HP15_2687 0.19 -2.2 2,834,713 + HP15_2687 0.31 -0.5 2,834,778 + HP15_2687 0.38 +0.1 2,834,877 - HP15_2687 0.48 +1.5 2,835,309 + -0.3 2,835,351 + +0.9 2,835,359 - +0.3
Or see this region's nucleotide sequence