Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2533 and HP15_2534 are separated by 18 nucleotides HP15_2534 and HP15_2535 are separated by 21 nucleotides HP15_2535 and HP15_2536 are separated by 106 nucleotides
HP15_2533: HP15_2533 - conserved hypothetical protein, secreted, at 2,677,937 to 2,679,001
_2533
HP15_2534: HP15_2534 - hypothetical protein, at 2,679,020 to 2,679,184
_2534
HP15_2535: HP15_2535 - transcriptional regulator, TetR family, at 2,679,206 to 2,679,865
_2535
HP15_2536: HP15_2536 - protein containing DUF1446, at 2,679,972 to 2,681,768
_2536
Position (kb)
2679
2680 Strain fitness (log2 ratio)
-1
0
1 at 2678.257 kb on - strand, within HP15_2533 at 2678.521 kb on - strand, within HP15_2533 at 2678.777 kb on - strand, within HP15_2533 at 2678.790 kb on + strand, within HP15_2533 at 2678.880 kb on - strand, within HP15_2533 at 2678.880 kb on - strand, within HP15_2533 at 2678.954 kb on - strand at 2678.954 kb on - strand at 2679.056 kb on - strand, within HP15_2534 at 2679.150 kb on + strand, within HP15_2534 at 2679.250 kb on + strand at 2679.250 kb on + strand at 2679.278 kb on - strand, within HP15_2535 at 2679.306 kb on + strand, within HP15_2535 at 2679.314 kb on - strand, within HP15_2535 at 2679.551 kb on + strand, within HP15_2535 at 2679.551 kb on + strand, within HP15_2535 at 2679.559 kb on - strand, within HP15_2535 at 2679.569 kb on - strand, within HP15_2535 at 2679.576 kb on + strand, within HP15_2535 at 2679.576 kb on + strand, within HP15_2535 at 2679.823 kb on - strand at 2679.983 kb on + strand at 2679.991 kb on - strand at 2680.143 kb on + strand at 2680.143 kb on + strand at 2680.143 kb on + strand at 2680.151 kb on - strand at 2680.158 kb on - strand, within HP15_2536 at 2680.199 kb on + strand, within HP15_2536 at 2680.460 kb on - strand, within HP15_2536 at 2680.605 kb on + strand, within HP15_2536 at 2680.611 kb on - strand, within HP15_2536 at 2680.613 kb on - strand, within HP15_2536 at 2680.613 kb on - strand, within HP15_2536 at 2680.830 kb on + strand, within HP15_2536 at 2680.831 kb on + strand, within HP15_2536 at 2680.839 kb on - strand, within HP15_2536 at 2680.839 kb on - strand, within HP15_2536 at 2680.841 kb on + strand, within HP15_2536
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,678,257 - HP15_2533 0.30 -0.5 2,678,521 - HP15_2533 0.55 +0.3 2,678,777 - HP15_2533 0.79 +0.1 2,678,790 + HP15_2533 0.80 +0.8 2,678,880 - HP15_2533 0.89 -0.1 2,678,880 - HP15_2533 0.89 +0.0 2,678,954 - -0.6 2,678,954 - -0.4 2,679,056 - HP15_2534 0.22 +0.4 2,679,150 + HP15_2534 0.79 -1.1 2,679,250 + +0.3 2,679,250 + +1.3 2,679,278 - HP15_2535 0.11 -0.5 2,679,306 + HP15_2535 0.15 -0.5 2,679,314 - HP15_2535 0.16 -0.7 2,679,551 + HP15_2535 0.52 +0.5 2,679,551 + HP15_2535 0.52 -1.7 2,679,559 - HP15_2535 0.53 -0.8 2,679,569 - HP15_2535 0.55 -1.0 2,679,576 + HP15_2535 0.56 -0.8 2,679,576 + HP15_2535 0.56 -0.6 2,679,823 - -0.2 2,679,983 + -0.2 2,679,991 - -0.6 2,680,143 + +0.6 2,680,143 + +0.1 2,680,143 + +0.6 2,680,151 - +0.6 2,680,158 - HP15_2536 0.10 +1.0 2,680,199 + HP15_2536 0.13 +0.7 2,680,460 - HP15_2536 0.27 +0.9 2,680,605 + HP15_2536 0.35 +0.7 2,680,611 - HP15_2536 0.36 +1.1 2,680,613 - HP15_2536 0.36 -0.5 2,680,613 - HP15_2536 0.36 -0.7 2,680,830 + HP15_2536 0.48 -0.4 2,680,831 + HP15_2536 0.48 -0.5 2,680,839 - HP15_2536 0.48 +0.5 2,680,839 - HP15_2536 0.48 +0.4 2,680,841 + HP15_2536 0.48 -0.6
Or see this region's nucleotide sequence