Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2465 and HP15_2466 are separated by 185 nucleotides HP15_2466 and HP15_2467 are separated by 444 nucleotides
HP15_2465: HP15_2465 - tRNA guanosine-2'-O-methyltransferase, at 2,605,002 to 2,605,739
_2465
HP15_2466: HP15_2466 - tRNA-i(6)A37 thiotransferase enzyme MiaB, at 2,605,925 to 2,607,271
_2466
HP15_2467: HP15_2467 - PhoH family protein, at 2,607,716 to 2,608,699
_2467
Position (kb)
2605
2606
2607
2608 Strain fitness (log2 ratio)
-2
-1
0
1 at 2605.031 kb on + strand at 2605.091 kb on - strand, within HP15_2465 at 2605.091 kb on - strand, within HP15_2465 at 2605.156 kb on + strand, within HP15_2465 at 2605.258 kb on + strand, within HP15_2465 at 2605.266 kb on - strand, within HP15_2465 at 2605.281 kb on + strand, within HP15_2465 at 2605.373 kb on - strand, within HP15_2465 at 2605.388 kb on + strand, within HP15_2465 at 2605.416 kb on - strand, within HP15_2465 at 2605.446 kb on + strand, within HP15_2465 at 2605.463 kb on + strand, within HP15_2465 at 2605.942 kb on + strand at 2605.949 kb on + strand at 2605.997 kb on - strand at 2605.997 kb on - strand at 2606.198 kb on + strand, within HP15_2466 at 2606.287 kb on + strand, within HP15_2466 at 2606.295 kb on + strand, within HP15_2466 at 2606.295 kb on - strand, within HP15_2466 at 2606.450 kb on - strand, within HP15_2466 at 2606.677 kb on - strand, within HP15_2466 at 2606.734 kb on - strand, within HP15_2466 at 2606.767 kb on + strand, within HP15_2466 at 2606.888 kb on - strand, within HP15_2466 at 2607.070 kb on + strand, within HP15_2466 at 2607.106 kb on + strand, within HP15_2466 at 2607.106 kb on + strand, within HP15_2466 at 2607.114 kb on - strand, within HP15_2466 at 2607.315 kb on + strand at 2607.316 kb on + strand at 2607.466 kb on + strand at 2607.478 kb on + strand at 2607.520 kb on - strand at 2607.585 kb on + strand at 2608.025 kb on + strand, within HP15_2467 at 2608.033 kb on + strand, within HP15_2467 at 2608.041 kb on - strand, within HP15_2467
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,605,031 + +0.3 2,605,091 - HP15_2465 0.12 +0.8 2,605,091 - HP15_2465 0.12 +1.1 2,605,156 + HP15_2465 0.21 -0.7 2,605,258 + HP15_2465 0.35 -0.5 2,605,266 - HP15_2465 0.36 +0.5 2,605,281 + HP15_2465 0.38 -0.4 2,605,373 - HP15_2465 0.50 +0.7 2,605,388 + HP15_2465 0.52 -0.0 2,605,416 - HP15_2465 0.56 -0.4 2,605,446 + HP15_2465 0.60 -0.8 2,605,463 + HP15_2465 0.62 +0.6 2,605,942 + -1.3 2,605,949 + +0.1 2,605,997 - -1.2 2,605,997 - -0.9 2,606,198 + HP15_2466 0.20 -1.4 2,606,287 + HP15_2466 0.27 -1.1 2,606,295 + HP15_2466 0.27 -0.7 2,606,295 - HP15_2466 0.27 +0.1 2,606,450 - HP15_2466 0.39 +0.3 2,606,677 - HP15_2466 0.56 -0.8 2,606,734 - HP15_2466 0.60 -0.3 2,606,767 + HP15_2466 0.63 -0.3 2,606,888 - HP15_2466 0.71 +0.2 2,607,070 + HP15_2466 0.85 +0.2 2,607,106 + HP15_2466 0.88 -1.7 2,607,106 + HP15_2466 0.88 -0.4 2,607,114 - HP15_2466 0.88 -0.8 2,607,315 + -0.2 2,607,316 + -0.4 2,607,466 + -1.9 2,607,478 + -0.4 2,607,520 - +0.1 2,607,585 + -0.8 2,608,025 + HP15_2467 0.31 +0.2 2,608,033 + HP15_2467 0.32 -0.3 2,608,041 - HP15_2467 0.33 +1.3
Or see this region's nucleotide sequence