Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2459 and HP15_2460 are separated by 44 nucleotides HP15_2460 and HP15_2461 are separated by 151 nucleotides HP15_2461 and HP15_2462 are separated by 160 nucleotides HP15_2462 and HP15_2463 are separated by 1 nucleotides
HP15_2459: HP15_2459 - MiaB-like tRNA modifying enzyme YliG, at 2,598,609 to 2,599,955
_2459
HP15_2460: HP15_2460 - hypothetical protein, at 2,600,000 to 2,600,119
_2460
HP15_2461: HP15_2461 - type IV pilus assembly PilZ, at 2,600,271 to 2,600,681
_2461
HP15_2462: HP15_2462 - sulfate adenylyltransferase, small subunit, at 2,600,842 to 2,601,750
_2462
HP15_2463: HP15_2463 - sulfate adenylyltransferase, large subunit, at 2,601,752 to 2,603,410
_2463
Position (kb)
2600
2601
2602 Strain fitness (log2 ratio)
-2
-1
0
1 at 2599.848 kb on - strand at 2599.848 kb on - strand at 2599.848 kb on - strand at 2599.885 kb on + strand at 2599.936 kb on - strand at 2599.949 kb on - strand at 2600.032 kb on - strand, within HP15_2460 at 2600.263 kb on - strand at 2600.376 kb on + strand, within HP15_2461 at 2600.453 kb on + strand, within HP15_2461 at 2600.550 kb on + strand, within HP15_2461 at 2600.578 kb on + strand, within HP15_2461 at 2600.675 kb on + strand at 2600.718 kb on - strand at 2600.775 kb on - strand at 2600.798 kb on + strand at 2600.816 kb on - strand at 2600.966 kb on - strand, within HP15_2462 at 2600.977 kb on - strand, within HP15_2462 at 2600.977 kb on - strand, within HP15_2462 at 2600.980 kb on + strand, within HP15_2462 at 2601.087 kb on - strand, within HP15_2462 at 2601.092 kb on + strand, within HP15_2462 at 2601.124 kb on + strand, within HP15_2462 at 2601.159 kb on - strand, within HP15_2462 at 2601.234 kb on + strand, within HP15_2462 at 2601.301 kb on + strand, within HP15_2462 at 2601.372 kb on + strand at 2601.380 kb on - strand, within HP15_2462 at 2601.390 kb on - strand, within HP15_2462 at 2601.415 kb on - strand, within HP15_2462 at 2601.598 kb on + strand, within HP15_2462 at 2601.603 kb on + strand, within HP15_2462 at 2601.747 kb on - strand at 2601.762 kb on + strand at 2601.770 kb on - strand at 2601.785 kb on + strand at 2601.795 kb on + strand at 2601.795 kb on + strand at 2601.966 kb on + strand, within HP15_2463 at 2602.023 kb on + strand, within HP15_2463 at 2602.102 kb on - strand, within HP15_2463 at 2602.172 kb on + strand, within HP15_2463 at 2602.319 kb on + strand, within HP15_2463 at 2602.346 kb on + strand, within HP15_2463 at 2602.360 kb on + strand, within HP15_2463 at 2602.440 kb on + strand, within HP15_2463 at 2602.578 kb on - strand, within HP15_2463 at 2602.585 kb on - strand, within HP15_2463 at 2602.650 kb on - strand, within HP15_2463
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,599,848 - -1.1 2,599,848 - -1.9 2,599,848 - -0.0 2,599,885 + -0.5 2,599,936 - -0.3 2,599,949 - +0.7 2,600,032 - HP15_2460 0.27 -0.7 2,600,263 - +0.2 2,600,376 + HP15_2461 0.26 +0.1 2,600,453 + HP15_2461 0.44 +0.0 2,600,550 + HP15_2461 0.68 +0.1 2,600,578 + HP15_2461 0.75 -0.2 2,600,675 + -0.4 2,600,718 - +0.1 2,600,775 - -0.0 2,600,798 + +0.2 2,600,816 - -1.1 2,600,966 - HP15_2462 0.14 +0.2 2,600,977 - HP15_2462 0.15 +0.1 2,600,977 - HP15_2462 0.15 +1.5 2,600,980 + HP15_2462 0.15 -0.9 2,601,087 - HP15_2462 0.27 -1.1 2,601,092 + HP15_2462 0.28 -0.2 2,601,124 + HP15_2462 0.31 -2.6 2,601,159 - HP15_2462 0.35 -1.5 2,601,234 + HP15_2462 0.43 -1.5 2,601,301 + HP15_2462 0.50 -0.1 2,601,372 + -1.1 2,601,380 - HP15_2462 0.59 -0.7 2,601,390 - HP15_2462 0.60 -1.2 2,601,415 - HP15_2462 0.63 -0.4 2,601,598 + HP15_2462 0.83 -1.1 2,601,603 + HP15_2462 0.84 -0.6 2,601,747 - -2.1 2,601,762 + -1.3 2,601,770 - -1.2 2,601,785 + -0.8 2,601,795 + +0.1 2,601,795 + -0.4 2,601,966 + HP15_2463 0.13 -1.7 2,602,023 + HP15_2463 0.16 -0.9 2,602,102 - HP15_2463 0.21 -1.6 2,602,172 + HP15_2463 0.25 -0.3 2,602,319 + HP15_2463 0.34 -1.3 2,602,346 + HP15_2463 0.36 -1.7 2,602,360 + HP15_2463 0.37 -1.6 2,602,440 + HP15_2463 0.41 -1.0 2,602,578 - HP15_2463 0.50 -0.9 2,602,585 - HP15_2463 0.50 +0.2 2,602,650 - HP15_2463 0.54 -0.1
Or see this region's nucleotide sequence