Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2440 and HP15_2441 overlap by 23 nucleotides HP15_2441 and HP15_2442 are separated by 150 nucleotides HP15_2442 and HP15_2443 are separated by 100 nucleotides HP15_2443 and HP15_2444 are separated by 87 nucleotides HP15_2444 and HP15_2445 are separated by 22 nucleotides
HP15_2440: HP15_2440 - OstA family protein, at 2,578,484 to 2,579,050
_2440
HP15_2441: HP15_2441 - protein containing ABC transporter-like domain, at 2,579,028 to 2,579,753
_2441
HP15_2442: HP15_2442 - RNA polymerase, sigma 54 subunit, RpoN, at 2,579,904 to 2,581,430
_2442
HP15_2443: HP15_2443 - sigma 54 modulation protein/ribosomal protein S30EA, at 2,581,531 to 2,581,839
_2443
HP15_2444: HP15_2444 - PTS IIA-like nitrogen-regulatory protein PtsN, at 2,581,927 to 2,582,397
_2444
HP15_2445: HP15_2445 - protein yhbJ, at 2,582,420 to 2,583,310
_2445
Position (kb)
2579
2580
2581
2582 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2580.063 kb on - strand, within HP15_2442 at 2580.401 kb on - strand, within HP15_2442 at 2580.510 kb on - strand, within HP15_2442 at 2580.635 kb on - strand, within HP15_2442 at 2580.665 kb on + strand, within HP15_2442 at 2580.920 kb on - strand, within HP15_2442 at 2581.016 kb on + strand, within HP15_2442 at 2581.016 kb on + strand, within HP15_2442 at 2581.386 kb on + strand at 2581.452 kb on - strand at 2581.556 kb on + strand at 2581.710 kb on + strand, within HP15_2443
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,580,063 - HP15_2442 0.10 -0.4 2,580,401 - HP15_2442 0.33 -0.4 2,580,510 - HP15_2442 0.40 -0.6 2,580,635 - HP15_2442 0.48 -1.0 2,580,665 + HP15_2442 0.50 -0.8 2,580,920 - HP15_2442 0.67 -0.6 2,581,016 + HP15_2442 0.73 -2.1 2,581,016 + HP15_2442 0.73 -0.9 2,581,386 + +0.5 2,581,452 - -1.6 2,581,556 + +1.8 2,581,710 + HP15_2443 0.58 +0.0
Or see this region's nucleotide sequence