Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2423 and HP15_2424 are separated by 129 nucleotides HP15_2424 and HP15_2425 are separated by 195 nucleotides HP15_2425 and HP15_2426 are separated by 227 nucleotides
HP15_2423: HP15_2423 - acyl-CoA thiolase, at 2,564,023 to 2,565,162
_2423
HP15_2424: HP15_2424 - AFG1-family ATPase, at 2,565,292 to 2,566,425
_2424
HP15_2425: HP15_2425 - membrane or secreted protein containing DUF1043, at 2,566,621 to 2,567,061
_2425
HP15_2426: HP15_2426 - NGG1p interacting factor 3, NIF3, at 2,567,289 to 2,568,032
_2426
Position (kb)
2565
2566
2567 Strain fitness (log2 ratio)
-2
-1
0
1 at 2564.345 kb on + strand, within HP15_2423 at 2564.391 kb on - strand, within HP15_2423 at 2564.447 kb on - strand, within HP15_2423 at 2564.609 kb on + strand, within HP15_2423 at 2564.649 kb on + strand, within HP15_2423 at 2564.654 kb on - strand, within HP15_2423 at 2564.686 kb on + strand, within HP15_2423 at 2564.694 kb on - strand, within HP15_2423 at 2564.751 kb on + strand, within HP15_2423 at 2564.759 kb on - strand, within HP15_2423 at 2564.767 kb on + strand, within HP15_2423 at 2564.791 kb on + strand, within HP15_2423 at 2564.848 kb on - strand, within HP15_2423 at 2564.899 kb on - strand, within HP15_2423 at 2565.078 kb on - strand at 2565.085 kb on + strand at 2565.119 kb on - strand at 2565.134 kb on - strand at 2565.136 kb on + strand at 2565.218 kb on + strand at 2565.257 kb on + strand at 2565.265 kb on - strand at 2565.265 kb on - strand at 2565.265 kb on - strand at 2565.272 kb on + strand at 2565.301 kb on + strand at 2565.316 kb on - strand at 2566.288 kb on + strand, within HP15_2424 at 2566.392 kb on - strand at 2566.431 kb on - strand at 2566.571 kb on - strand at 2566.684 kb on - strand, within HP15_2425 at 2566.903 kb on - strand, within HP15_2425 at 2566.988 kb on - strand, within HP15_2425 at 2567.101 kb on + strand at 2567.327 kb on + strand at 2567.381 kb on - strand, within HP15_2426 at 2567.410 kb on + strand, within HP15_2426
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,564,345 + HP15_2423 0.28 +0.5 2,564,391 - HP15_2423 0.32 -1.4 2,564,447 - HP15_2423 0.37 +0.7 2,564,609 + HP15_2423 0.51 +0.8 2,564,649 + HP15_2423 0.55 -0.3 2,564,654 - HP15_2423 0.55 +0.2 2,564,686 + HP15_2423 0.58 -0.9 2,564,694 - HP15_2423 0.59 +0.6 2,564,751 + HP15_2423 0.64 -0.3 2,564,759 - HP15_2423 0.65 -0.5 2,564,767 + HP15_2423 0.65 +1.0 2,564,791 + HP15_2423 0.67 -0.2 2,564,848 - HP15_2423 0.72 -1.0 2,564,899 - HP15_2423 0.77 +1.2 2,565,078 - -0.5 2,565,085 + +0.1 2,565,119 - -0.3 2,565,134 - -0.6 2,565,136 + -0.3 2,565,218 + -0.6 2,565,257 + -0.2 2,565,265 - -0.5 2,565,265 - -0.6 2,565,265 - -0.4 2,565,272 + +0.5 2,565,301 + -0.1 2,565,316 - -0.1 2,566,288 + HP15_2424 0.88 -2.0 2,566,392 - -0.9 2,566,431 - -0.7 2,566,571 - -0.6 2,566,684 - HP15_2425 0.14 +0.1 2,566,903 - HP15_2425 0.64 +0.2 2,566,988 - HP15_2425 0.83 -0.5 2,567,101 + +0.5 2,567,327 + -0.3 2,567,381 - HP15_2426 0.12 +0.4 2,567,410 + HP15_2426 0.16 +0.0
Or see this region's nucleotide sequence