Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2413 and HP15_2414 overlap by 1 nucleotides HP15_2414 and HP15_2415 are separated by 43 nucleotides HP15_2415 and HP15_2416 are separated by 95 nucleotides HP15_2416 and HP15_2417 are separated by 112 nucleotides
HP15_2413: HP15_2413 - conserved hypothetical protein, secreted, at 2,547,991 to 2,549,085
_2413
HP15_2414: HP15_2414 - protein containing uncharacterized protein family UPF0066 domains, at 2,549,085 to 2,549,828
_2414
HP15_2415: HP15_2415 - sensory box sensor histidine kinase/response regulator, at 2,549,872 to 2,552,223
_2415
HP15_2416: HP15_2416 - zinc-binding protein, at 2,552,319 to 2,552,474
_2416
HP15_2417: HP15_2417 - YfaZ family protein, at 2,552,587 to 2,553,144
_2417
Position (kb)
2549
2550
2551
2552
2553 Strain fitness (log2 ratio)
-1
0
1 at 2548.898 kb on - strand, within HP15_2413 at 2549.009 kb on + strand at 2549.017 kb on - strand at 2549.185 kb on + strand, within HP15_2414 at 2549.415 kb on - strand, within HP15_2414 at 2549.556 kb on + strand, within HP15_2414 at 2549.564 kb on - strand, within HP15_2414 at 2549.672 kb on + strand, within HP15_2414 at 2549.748 kb on + strand, within HP15_2414 at 2549.906 kb on - strand at 2549.936 kb on + strand at 2550.054 kb on - strand at 2550.177 kb on - strand, within HP15_2415 at 2550.306 kb on - strand, within HP15_2415 at 2550.316 kb on - strand, within HP15_2415 at 2550.339 kb on - strand, within HP15_2415 at 2550.702 kb on - strand, within HP15_2415 at 2550.702 kb on - strand, within HP15_2415 at 2550.742 kb on - strand, within HP15_2415 at 2551.056 kb on - strand, within HP15_2415 at 2551.058 kb on + strand, within HP15_2415 at 2551.205 kb on + strand, within HP15_2415 at 2551.213 kb on - strand, within HP15_2415 at 2551.405 kb on - strand, within HP15_2415 at 2551.607 kb on - strand, within HP15_2415 at 2551.860 kb on + strand, within HP15_2415 at 2551.936 kb on + strand, within HP15_2415 at 2552.004 kb on - strand at 2552.023 kb on - strand at 2552.298 kb on + strand at 2552.364 kb on - strand, within HP15_2416 at 2552.448 kb on - strand, within HP15_2416 at 2552.468 kb on + strand at 2552.476 kb on - strand at 2552.733 kb on + strand, within HP15_2417 at 2552.862 kb on - strand, within HP15_2417
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,548,898 - HP15_2413 0.83 -0.6 2,549,009 + -1.0 2,549,017 - +0.8 2,549,185 + HP15_2414 0.13 +0.5 2,549,415 - HP15_2414 0.44 +0.3 2,549,556 + HP15_2414 0.63 +0.9 2,549,564 - HP15_2414 0.64 -1.0 2,549,672 + HP15_2414 0.79 -0.5 2,549,748 + HP15_2414 0.89 +0.5 2,549,906 - -1.4 2,549,936 + -0.7 2,550,054 - -0.9 2,550,177 - HP15_2415 0.13 -0.5 2,550,306 - HP15_2415 0.18 -0.4 2,550,316 - HP15_2415 0.19 -1.1 2,550,339 - HP15_2415 0.20 +0.0 2,550,702 - HP15_2415 0.35 -0.0 2,550,702 - HP15_2415 0.35 -0.4 2,550,742 - HP15_2415 0.37 -1.0 2,551,056 - HP15_2415 0.50 +0.0 2,551,058 + HP15_2415 0.50 +0.3 2,551,205 + HP15_2415 0.57 -1.1 2,551,213 - HP15_2415 0.57 -0.1 2,551,405 - HP15_2415 0.65 -1.3 2,551,607 - HP15_2415 0.74 -1.0 2,551,860 + HP15_2415 0.85 +0.1 2,551,936 + HP15_2415 0.88 -0.1 2,552,004 - -1.0 2,552,023 - -0.5 2,552,298 + -0.2 2,552,364 - HP15_2416 0.29 -0.2 2,552,448 - HP15_2416 0.83 +0.6 2,552,468 + +0.5 2,552,476 - +0.2 2,552,733 + HP15_2417 0.26 -0.7 2,552,862 - HP15_2417 0.49 -1.1
Or see this region's nucleotide sequence