Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2166 and HP15_2167 are separated by 204 nucleotides HP15_2167 and HP15_2168 are separated by 185 nucleotides
HP15_2166: HP15_2166 - glycerol kinase, at 2,273,876 to 2,275,348
_2166
HP15_2167: HP15_2167 - transcriptional repressor for the dissimilation of sn-glycerol 3-phosphate, DeoR family protein, at 2,275,553 to 2,276,309
_2167
HP15_2168: HP15_2168 - FAD dependent oxidoreductase, at 2,276,495 to 2,278,018
_2168
Position (kb)
2275
2276
2277 Strain fitness (log2 ratio)
-1
0
1 at 2274.583 kb on + strand, within HP15_2166 at 2274.632 kb on + strand, within HP15_2166 at 2274.728 kb on + strand, within HP15_2166 at 2274.784 kb on + strand, within HP15_2166 at 2274.810 kb on + strand, within HP15_2166 at 2274.851 kb on + strand, within HP15_2166 at 2274.990 kb on - strand, within HP15_2166 at 2274.996 kb on - strand, within HP15_2166 at 2274.996 kb on - strand, within HP15_2166 at 2275.027 kb on - strand, within HP15_2166 at 2275.116 kb on - strand, within HP15_2166 at 2275.127 kb on + strand, within HP15_2166 at 2275.300 kb on - strand at 2275.300 kb on - strand at 2275.324 kb on - strand at 2275.614 kb on + strand at 2275.643 kb on - strand, within HP15_2167 at 2275.649 kb on + strand, within HP15_2167 at 2275.649 kb on + strand, within HP15_2167 at 2275.708 kb on + strand, within HP15_2167 at 2275.828 kb on - strand, within HP15_2167 at 2276.029 kb on - strand, within HP15_2167 at 2276.188 kb on - strand, within HP15_2167 at 2276.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,274,583 + HP15_2166 0.48 -0.2 2,274,632 + HP15_2166 0.51 +0.8 2,274,728 + HP15_2166 0.58 +0.4 2,274,784 + HP15_2166 0.62 -0.1 2,274,810 + HP15_2166 0.63 +1.0 2,274,851 + HP15_2166 0.66 -0.4 2,274,990 - HP15_2166 0.76 +0.1 2,274,996 - HP15_2166 0.76 -0.3 2,274,996 - HP15_2166 0.76 -1.0 2,275,027 - HP15_2166 0.78 +1.0 2,275,116 - HP15_2166 0.84 -0.4 2,275,127 + HP15_2166 0.85 -0.1 2,275,300 - +0.1 2,275,300 - -0.5 2,275,324 - +1.2 2,275,614 + -0.9 2,275,643 - HP15_2167 0.12 -0.2 2,275,649 + HP15_2167 0.13 +1.4 2,275,649 + HP15_2167 0.13 -0.2 2,275,708 + HP15_2167 0.20 +0.1 2,275,828 - HP15_2167 0.36 +0.2 2,276,029 - HP15_2167 0.63 +0.4 2,276,188 - HP15_2167 0.84 -0.8 2,276,295 - -1.1
Or see this region's nucleotide sequence