Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2017 and HP15_2018 overlap by 14 nucleotides HP15_2018 and HP15_2019 are separated by 9 nucleotides HP15_2019 and HP15_2020 are separated by 33 nucleotides
HP15_2017: HP15_2017 - branched-chain amino acid ABC transporter ATP-binding protein, at 2,118,142 to 2,118,945
_2017
HP15_2018: HP15_2018 - branched-chain amino acid ABC transporter, ATP-binding protein, at 2,118,932 to 2,119,588
_2018
HP15_2019: HP15_2019 - iron-containing alcohol dehydrogenase, at 2,119,598 to 2,120,755
_2019
HP15_2020: HP15_2020 - succinate-semialdehyde dehydrogenase I, at 2,120,789 to 2,122,258
_2020
Position (kb)
2119
2120
2121 Strain fitness (log2 ratio)
-2
-1
0
1 at 2118.601 kb on + strand, within HP15_2017 at 2118.726 kb on - strand, within HP15_2017 at 2118.774 kb on + strand, within HP15_2017 at 2118.774 kb on + strand, within HP15_2017 at 2118.782 kb on - strand, within HP15_2017 at 2118.808 kb on + strand, within HP15_2017 at 2118.833 kb on + strand, within HP15_2017 at 2119.041 kb on + strand, within HP15_2018 at 2119.232 kb on + strand, within HP15_2018 at 2119.330 kb on - strand, within HP15_2018 at 2119.358 kb on + strand, within HP15_2018 at 2119.366 kb on - strand, within HP15_2018 at 2119.366 kb on - strand, within HP15_2018 at 2119.366 kb on - strand, within HP15_2018 at 2119.417 kb on - strand, within HP15_2018 at 2119.495 kb on - strand, within HP15_2018 at 2119.503 kb on - strand, within HP15_2018 at 2119.523 kb on + strand at 2119.843 kb on - strand, within HP15_2019 at 2119.970 kb on - strand, within HP15_2019 at 2119.972 kb on + strand, within HP15_2019 at 2119.985 kb on + strand, within HP15_2019 at 2120.248 kb on - strand, within HP15_2019 at 2120.250 kb on + strand, within HP15_2019 at 2120.332 kb on + strand, within HP15_2019 at 2120.332 kb on + strand, within HP15_2019 at 2120.482 kb on + strand, within HP15_2019 at 2120.585 kb on - strand, within HP15_2019 at 2120.610 kb on + strand, within HP15_2019 at 2120.649 kb on - strand at 2120.701 kb on + strand at 2120.703 kb on + strand at 2120.824 kb on - strand at 2120.838 kb on - strand at 2120.867 kb on + strand at 2120.953 kb on + strand, within HP15_2020 at 2121.076 kb on - strand, within HP15_2020 at 2121.088 kb on + strand, within HP15_2020 at 2121.096 kb on - strand, within HP15_2020 at 2121.160 kb on + strand, within HP15_2020 at 2121.160 kb on + strand, within HP15_2020 at 2121.168 kb on - strand, within HP15_2020 at 2121.170 kb on + strand, within HP15_2020 at 2121.257 kb on + strand, within HP15_2020 at 2121.323 kb on + strand, within HP15_2020 at 2121.336 kb on - strand, within HP15_2020 at 2121.461 kb on - strand, within HP15_2020 at 2121.501 kb on + strand, within HP15_2020 at 2121.649 kb on + strand, within HP15_2020 at 2121.659 kb on + strand, within HP15_2020 at 2121.667 kb on - strand, within HP15_2020 at 2121.701 kb on + strand, within HP15_2020 at 2121.735 kb on - strand, within HP15_2020
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,118,601 + HP15_2017 0.57 -0.2 2,118,726 - HP15_2017 0.73 +0.5 2,118,774 + HP15_2017 0.79 -0.6 2,118,774 + HP15_2017 0.79 -0.1 2,118,782 - HP15_2017 0.80 -0.2 2,118,808 + HP15_2017 0.83 +0.3 2,118,833 + HP15_2017 0.86 -0.0 2,119,041 + HP15_2018 0.17 -1.8 2,119,232 + HP15_2018 0.46 -0.9 2,119,330 - HP15_2018 0.61 +0.2 2,119,358 + HP15_2018 0.65 +0.9 2,119,366 - HP15_2018 0.66 -0.2 2,119,366 - HP15_2018 0.66 +0.9 2,119,366 - HP15_2018 0.66 -1.3 2,119,417 - HP15_2018 0.74 -0.3 2,119,495 - HP15_2018 0.86 -0.9 2,119,503 - HP15_2018 0.87 -0.3 2,119,523 + +1.2 2,119,843 - HP15_2019 0.21 +0.5 2,119,970 - HP15_2019 0.32 -0.4 2,119,972 + HP15_2019 0.32 -0.7 2,119,985 + HP15_2019 0.33 +0.8 2,120,248 - HP15_2019 0.56 -0.5 2,120,250 + HP15_2019 0.56 +0.1 2,120,332 + HP15_2019 0.63 -0.5 2,120,332 + HP15_2019 0.63 +0.5 2,120,482 + HP15_2019 0.76 +0.5 2,120,585 - HP15_2019 0.85 +1.2 2,120,610 + HP15_2019 0.87 +0.6 2,120,649 - -0.3 2,120,701 + +0.2 2,120,703 + +1.2 2,120,824 - -0.4 2,120,838 - -0.5 2,120,867 + -0.2 2,120,953 + HP15_2020 0.11 -0.3 2,121,076 - HP15_2020 0.20 +0.0 2,121,088 + HP15_2020 0.20 -0.9 2,121,096 - HP15_2020 0.21 -0.6 2,121,160 + HP15_2020 0.25 -0.5 2,121,160 + HP15_2020 0.25 +0.3 2,121,168 - HP15_2020 0.26 +0.1 2,121,170 + HP15_2020 0.26 +0.8 2,121,257 + HP15_2020 0.32 +0.8 2,121,323 + HP15_2020 0.36 -0.4 2,121,336 - HP15_2020 0.37 -0.5 2,121,461 - HP15_2020 0.46 -0.0 2,121,501 + HP15_2020 0.48 +0.6 2,121,649 + HP15_2020 0.59 -0.0 2,121,659 + HP15_2020 0.59 +0.4 2,121,667 - HP15_2020 0.60 +0.7 2,121,701 + HP15_2020 0.62 -0.1 2,121,735 - HP15_2020 0.64 -0.1
Or see this region's nucleotide sequence