Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1972 and HP15_1973 overlap by 11 nucleotides HP15_1973 and HP15_1974 are separated by 1 nucleotides HP15_1974 and HP15_1975 overlap by 4 nucleotides HP15_1975 and HP15_1976 are separated by 107 nucleotides
HP15_1972: HP15_1972 - fibronectin, type III domain protein-like protein, at 2,089,769 to 2,090,875
_1972
HP15_1973: HP15_1973 - hypothetical protein, at 2,090,865 to 2,090,984
_1973
HP15_1974: HP15_1974 - hypothetical protein, at 2,090,986 to 2,091,447
_1974
HP15_1975: HP15_1975 - conserved hypothetical protein, at 2,091,444 to 2,091,581
_1975
HP15_1976: HP15_1976 - hypothetical protein, at 2,091,689 to 2,092,069
_1976
Position (kb)
2090
2091 Strain fitness (log2 ratio)
-2
-1
0
1 at 2089.885 kb on + strand, within HP15_1972 at 2089.893 kb on - strand, within HP15_1972 at 2089.893 kb on - strand, within HP15_1972 at 2089.968 kb on + strand, within HP15_1972 at 2090.083 kb on + strand, within HP15_1972 at 2090.083 kb on + strand, within HP15_1972 at 2090.083 kb on + strand, within HP15_1972 at 2090.091 kb on - strand, within HP15_1972 at 2090.091 kb on - strand, within HP15_1972 at 2090.124 kb on - strand, within HP15_1972 at 2090.344 kb on + strand, within HP15_1972 at 2090.362 kb on - strand, within HP15_1972 at 2090.559 kb on - strand, within HP15_1972 at 2090.573 kb on + strand, within HP15_1972 at 2090.695 kb on - strand, within HP15_1972 at 2090.775 kb on + strand at 2090.777 kb on + strand at 2090.783 kb on - strand at 2090.783 kb on - strand at 2090.783 kb on - strand at 2090.809 kb on - strand at 2090.828 kb on - strand at 2090.931 kb on - strand, within HP15_1973 at 2090.958 kb on + strand, within HP15_1973 at 2091.104 kb on + strand, within HP15_1974 at 2091.241 kb on - strand, within HP15_1974 at 2091.252 kb on + strand, within HP15_1974 at 2091.463 kb on + strand, within HP15_1975 at 2091.471 kb on - strand, within HP15_1975 at 2091.615 kb on + strand at 2091.660 kb on + strand at 2091.665 kb on + strand at 2091.673 kb on - strand at 2091.738 kb on + strand, within HP15_1976 at 2091.738 kb on + strand, within HP15_1976 at 2091.738 kb on + strand, within HP15_1976 at 2091.787 kb on + strand, within HP15_1976 at 2091.795 kb on - strand, within HP15_1976 at 2091.795 kb on - strand, within HP15_1976 at 2091.795 kb on - strand, within HP15_1976 at 2091.795 kb on - strand, within HP15_1976 at 2091.807 kb on + strand, within HP15_1976 at 2091.975 kb on + strand, within HP15_1976 at 2091.975 kb on + strand, within HP15_1976 at 2091.975 kb on + strand, within HP15_1976 at 2091.976 kb on - strand, within HP15_1976 at 2091.983 kb on - strand, within HP15_1976
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 2,089,885 + HP15_1972 0.10 +0.5 2,089,893 - HP15_1972 0.11 -0.6 2,089,893 - HP15_1972 0.11 +0.7 2,089,968 + HP15_1972 0.18 +0.0 2,090,083 + HP15_1972 0.28 +0.0 2,090,083 + HP15_1972 0.28 -0.4 2,090,083 + HP15_1972 0.28 -0.8 2,090,091 - HP15_1972 0.29 +0.5 2,090,091 - HP15_1972 0.29 +0.9 2,090,124 - HP15_1972 0.32 +0.5 2,090,344 + HP15_1972 0.52 +0.1 2,090,362 - HP15_1972 0.54 +0.9 2,090,559 - HP15_1972 0.71 -0.3 2,090,573 + HP15_1972 0.73 +0.4 2,090,695 - HP15_1972 0.84 -0.4 2,090,775 + -2.5 2,090,777 + +0.7 2,090,783 - -0.0 2,090,783 - -0.2 2,090,783 - +0.5 2,090,809 - +0.8 2,090,828 - +0.3 2,090,931 - HP15_1973 0.55 -0.6 2,090,958 + HP15_1973 0.78 +0.2 2,091,104 + HP15_1974 0.26 -1.3 2,091,241 - HP15_1974 0.55 +0.4 2,091,252 + HP15_1974 0.58 +0.4 2,091,463 + HP15_1975 0.14 -1.2 2,091,471 - HP15_1975 0.20 +0.5 2,091,615 + +1.3 2,091,660 + +1.4 2,091,665 + -0.4 2,091,673 - +1.0 2,091,738 + HP15_1976 0.13 +0.4 2,091,738 + HP15_1976 0.13 -0.2 2,091,738 + HP15_1976 0.13 +0.2 2,091,787 + HP15_1976 0.26 +0.5 2,091,795 - HP15_1976 0.28 +0.8 2,091,795 - HP15_1976 0.28 +1.2 2,091,795 - HP15_1976 0.28 +0.8 2,091,795 - HP15_1976 0.28 -0.2 2,091,807 + HP15_1976 0.31 -1.4 2,091,975 + HP15_1976 0.75 -0.4 2,091,975 + HP15_1976 0.75 +0.1 2,091,975 + HP15_1976 0.75 +0.8 2,091,976 - HP15_1976 0.75 -0.3 2,091,983 - HP15_1976 0.77 +0.5
Or see this region's nucleotide sequence