Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1859 and HP15_1860 are separated by 29 nucleotides HP15_1860 and HP15_1861 are separated by 143 nucleotides HP15_1861 and HP15_1862 are separated by 12 nucleotides
HP15_1859: HP15_1859 - patatin-like phospholipase domain-containing protein, at 1,961,853 to 1,963,340
_1859
HP15_1860: HP15_1860 - VacJ family lipoprotein, at 1,963,370 to 1,964,161
_1860
HP15_1861: HP15_1861 - thioesterase superfamily protein, at 1,964,305 to 1,964,655
_1861
HP15_1862: HP15_1862 - 3-oxoacyl-(acyl carrier protein) synthase III, at 1,964,668 to 1,965,789
_1862
Position (kb)
1963
1964
1965 Strain fitness (log2 ratio)
-1
0
1 at 1962.423 kb on - strand, within HP15_1859 at 1962.480 kb on + strand, within HP15_1859 at 1962.495 kb on + strand, within HP15_1859 at 1962.549 kb on + strand, within HP15_1859 at 1962.778 kb on - strand, within HP15_1859 at 1962.811 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.003 kb on + strand, within HP15_1859 at 1963.011 kb on - strand, within HP15_1859 at 1963.011 kb on - strand, within HP15_1859 at 1963.016 kb on - strand, within HP15_1859 at 1963.059 kb on + strand, within HP15_1859 at 1963.059 kb on + strand, within HP15_1859 at 1963.067 kb on - strand, within HP15_1859 at 1963.105 kb on - strand, within HP15_1859 at 1963.106 kb on - strand, within HP15_1859 at 1963.113 kb on - strand, within HP15_1859 at 1963.115 kb on - strand, within HP15_1859 at 1963.267 kb on + strand at 1963.348 kb on - strand at 1963.637 kb on + strand, within HP15_1860 at 1963.637 kb on + strand, within HP15_1860 at 1963.749 kb on - strand, within HP15_1860 at 1963.777 kb on + strand, within HP15_1860 at 1963.777 kb on + strand, within HP15_1860 at 1963.784 kb on + strand, within HP15_1860 at 1963.867 kb on + strand, within HP15_1860 at 1964.035 kb on - strand, within HP15_1860 at 1964.249 kb on + strand at 1964.274 kb on + strand at 1964.284 kb on - strand at 1964.343 kb on - strand, within HP15_1861 at 1964.381 kb on + strand, within HP15_1861 at 1964.425 kb on + strand, within HP15_1861 at 1964.503 kb on + strand, within HP15_1861 at 1964.808 kb on + strand, within HP15_1862 at 1964.855 kb on + strand, within HP15_1862 at 1964.856 kb on - strand, within HP15_1862 at 1964.882 kb on - strand, within HP15_1862 at 1965.128 kb on + strand, within HP15_1862
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,962,423 - HP15_1859 0.38 +0.4 1,962,480 + HP15_1859 0.42 -0.3 1,962,495 + HP15_1859 0.43 -0.0 1,962,549 + HP15_1859 0.47 +1.0 1,962,778 - HP15_1859 0.62 +0.1 1,962,811 + HP15_1859 0.64 +0.2 1,963,003 + HP15_1859 0.77 +0.1 1,963,003 + HP15_1859 0.77 -0.1 1,963,003 + HP15_1859 0.77 +0.5 1,963,003 + HP15_1859 0.77 -0.1 1,963,003 + HP15_1859 0.77 -0.2 1,963,003 + HP15_1859 0.77 -0.4 1,963,003 + HP15_1859 0.77 -0.2 1,963,003 + HP15_1859 0.77 -0.5 1,963,011 - HP15_1859 0.78 +0.3 1,963,011 - HP15_1859 0.78 +0.4 1,963,016 - HP15_1859 0.78 +1.0 1,963,059 + HP15_1859 0.81 +0.4 1,963,059 + HP15_1859 0.81 +0.0 1,963,067 - HP15_1859 0.82 -0.2 1,963,105 - HP15_1859 0.84 +0.2 1,963,106 - HP15_1859 0.84 +0.9 1,963,113 - HP15_1859 0.85 -0.3 1,963,115 - HP15_1859 0.85 +0.3 1,963,267 + -0.2 1,963,348 - -0.1 1,963,637 + HP15_1860 0.34 -0.2 1,963,637 + HP15_1860 0.34 -0.3 1,963,749 - HP15_1860 0.48 -1.3 1,963,777 + HP15_1860 0.51 -1.4 1,963,777 + HP15_1860 0.51 -0.7 1,963,784 + HP15_1860 0.52 -1.0 1,963,867 + HP15_1860 0.63 +0.5 1,964,035 - HP15_1860 0.84 -0.0 1,964,249 + +0.6 1,964,274 + +0.0 1,964,284 - +0.4 1,964,343 - HP15_1861 0.11 -0.2 1,964,381 + HP15_1861 0.22 -1.0 1,964,425 + HP15_1861 0.34 +0.7 1,964,503 + HP15_1861 0.56 +0.6 1,964,808 + HP15_1862 0.12 -0.0 1,964,855 + HP15_1862 0.17 +0.5 1,964,856 - HP15_1862 0.17 -0.5 1,964,882 - HP15_1862 0.19 -0.4 1,965,128 + HP15_1862 0.41 +0.2
Or see this region's nucleotide sequence