Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1832 and HP15_1833 are separated by 42 nucleotides HP15_1833 and HP15_1834 are separated by 16 nucleotides HP15_1834 and HP15_1835 are separated by 347 nucleotides
HP15_1832: HP15_1832 - 3-isopropylmalate dehydrogenase, at 1,931,640 to 1,932,731
_1832
HP15_1833: HP15_1833 - isopropylmalate isomerase small subunit, at 1,932,774 to 1,933,421
_1833
HP15_1834: HP15_1834 - protein containing 3-isopropylmalate dehydratase, large subunit region domain, at 1,933,438 to 1,934,856
_1834
HP15_1835: HP15_1835 - transcriptional regulator LysR family protein, at 1,935,204 to 1,936,154
_1835
Position (kb)
1933
1934
1935 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1932.449 kb on - strand, within HP15_1832 at 1932.584 kb on + strand, within HP15_1832 at 1932.711 kb on - strand at 1932.842 kb on - strand, within HP15_1833 at 1933.149 kb on + strand, within HP15_1833 at 1933.182 kb on + strand, within HP15_1833 at 1933.201 kb on + strand, within HP15_1833 at 1933.367 kb on + strand at 1933.423 kb on + strand at 1933.449 kb on - strand at 1933.547 kb on + strand at 1933.568 kb on + strand at 1933.666 kb on + strand, within HP15_1834 at 1933.726 kb on - strand, within HP15_1834 at 1933.873 kb on - strand, within HP15_1834 at 1934.062 kb on + strand, within HP15_1834 at 1934.331 kb on - strand, within HP15_1834 at 1934.424 kb on - strand, within HP15_1834 at 1934.439 kb on - strand, within HP15_1834 at 1934.445 kb on + strand, within HP15_1834 at 1934.546 kb on + strand, within HP15_1834 at 1934.649 kb on - strand, within HP15_1834 at 1934.734 kb on + strand at 1934.745 kb on + strand at 1934.836 kb on + strand at 1934.863 kb on + strand at 1934.871 kb on + strand at 1934.879 kb on - strand at 1935.003 kb on + strand at 1935.011 kb on - strand at 1935.164 kb on + strand at 1935.209 kb on - strand at 1935.249 kb on - strand at 1935.287 kb on - strand at 1935.486 kb on + strand, within HP15_1835 at 1935.543 kb on - strand, within HP15_1835 at 1935.639 kb on - strand, within HP15_1835 at 1935.664 kb on - strand, within HP15_1835 at 1935.841 kb on + strand, within HP15_1835
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,932,449 - HP15_1832 0.74 -1.0 1,932,584 + HP15_1832 0.86 -1.9 1,932,711 - -0.2 1,932,842 - HP15_1833 0.10 -2.5 1,933,149 + HP15_1833 0.58 -3.8 1,933,182 + HP15_1833 0.63 +1.3 1,933,201 + HP15_1833 0.66 -3.3 1,933,367 + -2.4 1,933,423 + -0.7 1,933,449 - -0.5 1,933,547 + -3.7 1,933,568 + -1.7 1,933,666 + HP15_1834 0.16 -3.6 1,933,726 - HP15_1834 0.20 -0.7 1,933,873 - HP15_1834 0.31 +1.1 1,934,062 + HP15_1834 0.44 -2.2 1,934,331 - HP15_1834 0.63 -0.2 1,934,424 - HP15_1834 0.69 -2.5 1,934,439 - HP15_1834 0.71 -0.9 1,934,445 + HP15_1834 0.71 -1.2 1,934,546 + HP15_1834 0.78 -2.8 1,934,649 - HP15_1834 0.85 -1.1 1,934,734 + -1.4 1,934,745 + -1.0 1,934,836 + -1.4 1,934,863 + -0.4 1,934,871 + -3.1 1,934,879 - -1.8 1,935,003 + +0.3 1,935,011 - +0.7 1,935,164 + -0.3 1,935,209 - +0.5 1,935,249 - +0.3 1,935,287 - +0.4 1,935,486 + HP15_1835 0.30 +0.5 1,935,543 - HP15_1835 0.36 -0.1 1,935,639 - HP15_1835 0.46 +0.4 1,935,664 - HP15_1835 0.48 +0.2 1,935,841 + HP15_1835 0.67 +0.4
Or see this region's nucleotide sequence