Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1831 and HP15_1832 are separated by 164 nucleotides HP15_1832 and HP15_1833 are separated by 42 nucleotides HP15_1833 and HP15_1834 are separated by 16 nucleotides
HP15_1831: HP15_1831 - aspartate-semialdehyde dehydrogenase, at 1,930,393 to 1,931,475
_1831
HP15_1832: HP15_1832 - 3-isopropylmalate dehydrogenase, at 1,931,640 to 1,932,731
_1832
HP15_1833: HP15_1833 - isopropylmalate isomerase small subunit, at 1,932,774 to 1,933,421
_1833
HP15_1834: HP15_1834 - protein containing 3-isopropylmalate dehydratase, large subunit region domain, at 1,933,438 to 1,934,856
_1834
Position (kb)
1931
1932
1933 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1931.635 kb on + strand at 1931.641 kb on - strand at 1931.902 kb on - strand, within HP15_1832 at 1932.029 kb on - strand, within HP15_1832 at 1932.062 kb on + strand, within HP15_1832 at 1932.225 kb on + strand, within HP15_1832 at 1932.233 kb on - strand, within HP15_1832 at 1932.234 kb on + strand, within HP15_1832 at 1932.386 kb on + strand, within HP15_1832 at 1932.421 kb on - strand, within HP15_1832 at 1932.449 kb on - strand, within HP15_1832 at 1932.584 kb on + strand, within HP15_1832 at 1932.711 kb on - strand at 1932.842 kb on - strand, within HP15_1833 at 1933.149 kb on + strand, within HP15_1833 at 1933.182 kb on + strand, within HP15_1833 at 1933.201 kb on + strand, within HP15_1833 at 1933.367 kb on + strand at 1933.423 kb on + strand at 1933.449 kb on - strand at 1933.547 kb on + strand at 1933.568 kb on + strand at 1933.666 kb on + strand, within HP15_1834 at 1933.726 kb on - strand, within HP15_1834
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,931,635 + -0.7 1,931,641 - -1.8 1,931,902 - HP15_1832 0.24 -1.4 1,932,029 - HP15_1832 0.36 -1.0 1,932,062 + HP15_1832 0.39 -2.5 1,932,225 + HP15_1832 0.54 -3.0 1,932,233 - HP15_1832 0.54 -2.1 1,932,234 + HP15_1832 0.54 -2.0 1,932,386 + HP15_1832 0.68 -1.3 1,932,421 - HP15_1832 0.72 -2.2 1,932,449 - HP15_1832 0.74 -1.0 1,932,584 + HP15_1832 0.86 -1.9 1,932,711 - -0.2 1,932,842 - HP15_1833 0.10 -2.5 1,933,149 + HP15_1833 0.58 -3.8 1,933,182 + HP15_1833 0.63 +1.3 1,933,201 + HP15_1833 0.66 -3.3 1,933,367 + -2.4 1,933,423 + -0.7 1,933,449 - -0.5 1,933,547 + -3.7 1,933,568 + -1.7 1,933,666 + HP15_1834 0.16 -3.6 1,933,726 - HP15_1834 0.20 -0.7
Or see this region's nucleotide sequence