Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1743 and HP15_1744 are separated by 15 nucleotides HP15_1744 and HP15_tRNA33 are separated by 193 nucleotides HP15_tRNA33 and HP15_1745 are separated by 75 nucleotides
HP15_1743: HP15_1743 - conserved hypothetical protein, at 1,839,647 to 1,842,152
_1743
HP15_1744: HP15_1744 - AAA ATPase central domain protein, at 1,842,168 to 1,843,142
_1744
HP15_tRNA33: HP15_tRNA33 - tRNA-Leu, at 1,843,336 to 1,843,422
_tRNA33
HP15_1745: HP15_1745 - methyltransferase type 11, at 1,843,498 to 1,844,151
_1745
Position (kb)
1842
1843
1844 Strain fitness (log2 ratio)
-1
0
1 at 1841.371 kb on + strand, within HP15_1743 at 1841.456 kb on - strand, within HP15_1743 at 1841.459 kb on + strand, within HP15_1743 at 1841.591 kb on - strand, within HP15_1743 at 1841.663 kb on - strand, within HP15_1743 at 1841.749 kb on - strand, within HP15_1743 at 1841.758 kb on - strand, within HP15_1743 at 1841.758 kb on - strand, within HP15_1743 at 1841.924 kb on + strand at 1841.932 kb on - strand at 1841.947 kb on + strand at 1841.974 kb on + strand at 1842.013 kb on - strand at 1842.013 kb on - strand at 1842.018 kb on - strand at 1842.411 kb on + strand, within HP15_1744 at 1842.691 kb on + strand, within HP15_1744 at 1843.117 kb on + strand at 1843.152 kb on - strand at 1843.311 kb on + strand at 1843.511 kb on - strand at 1843.513 kb on + strand at 1843.523 kb on + strand at 1843.576 kb on - strand, within HP15_1745 at 1843.576 kb on - strand, within HP15_1745 at 1843.634 kb on - strand, within HP15_1745 at 1843.634 kb on - strand, within HP15_1745 at 1843.787 kb on - strand, within HP15_1745 at 1843.813 kb on - strand, within HP15_1745 at 1843.815 kb on + strand, within HP15_1745 at 1843.815 kb on + strand, within HP15_1745 at 1843.815 kb on + strand, within HP15_1745 at 1843.815 kb on + strand, within HP15_1745 at 1843.815 kb on + strand, within HP15_1745 at 1843.823 kb on - strand, within HP15_1745 at 1843.823 kb on - strand, within HP15_1745 at 1843.823 kb on - strand, within HP15_1745 at 1843.823 kb on - strand, within HP15_1745 at 1843.823 kb on - strand, within HP15_1745 at 1843.835 kb on - strand, within HP15_1745 at 1843.835 kb on - strand, within HP15_1745 at 1843.919 kb on + strand, within HP15_1745 at 1843.921 kb on - strand, within HP15_1745 at 1843.941 kb on + strand, within HP15_1745 at 1844.123 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,841,371 + HP15_1743 0.69 -0.6 1,841,456 - HP15_1743 0.72 +0.4 1,841,459 + HP15_1743 0.72 -0.6 1,841,591 - HP15_1743 0.78 +0.3 1,841,663 - HP15_1743 0.80 +0.2 1,841,749 - HP15_1743 0.84 +0.4 1,841,758 - HP15_1743 0.84 +0.2 1,841,758 - HP15_1743 0.84 +0.2 1,841,924 + +0.4 1,841,932 - +0.3 1,841,947 + +0.1 1,841,974 + +0.3 1,842,013 - -0.5 1,842,013 - +0.9 1,842,018 - +0.2 1,842,411 + HP15_1744 0.25 -0.4 1,842,691 + HP15_1744 0.54 -0.6 1,843,117 + +0.2 1,843,152 - -0.1 1,843,311 + +1.2 1,843,511 - -1.2 1,843,513 + +0.7 1,843,523 + -0.2 1,843,576 - HP15_1745 0.12 +0.1 1,843,576 - HP15_1745 0.12 +0.4 1,843,634 - HP15_1745 0.21 +0.3 1,843,634 - HP15_1745 0.21 -0.8 1,843,787 - HP15_1745 0.44 -0.5 1,843,813 - HP15_1745 0.48 -0.1 1,843,815 + HP15_1745 0.48 -1.5 1,843,815 + HP15_1745 0.48 -0.5 1,843,815 + HP15_1745 0.48 -0.3 1,843,815 + HP15_1745 0.48 +0.3 1,843,815 + HP15_1745 0.48 +0.2 1,843,823 - HP15_1745 0.50 +0.9 1,843,823 - HP15_1745 0.50 +0.4 1,843,823 - HP15_1745 0.50 -0.4 1,843,823 - HP15_1745 0.50 +0.7 1,843,823 - HP15_1745 0.50 +0.4 1,843,835 - HP15_1745 0.52 +0.6 1,843,835 - HP15_1745 0.52 +0.1 1,843,919 + HP15_1745 0.64 +1.3 1,843,921 - HP15_1745 0.65 -0.0 1,843,941 + HP15_1745 0.68 +0.3 1,844,123 - +0.3
Or see this region's nucleotide sequence