Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1623 and HP15_1624 are separated by 30 nucleotides HP15_1624 and HP15_1625 are separated by 193 nucleotides HP15_1625 and HP15_1626 are separated by 167 nucleotides HP15_1626 and HP15_1627 are separated by 170 nucleotides HP15_1627 and HP15_1628 are separated by 251 nucleotides
HP15_1623: HP15_1623 - conserved hypothetical protein, at 1,720,662 to 1,723,118
_1623
HP15_1624: HP15_1624 - YebG, at 1,723,149 to 1,723,376
_1624
HP15_1625: HP15_1625 - 3-oxoacyl-(acyl carrier protein) synthase III, at 1,723,570 to 1,724,538
_1625
HP15_1626: HP15_1626 - conserved hypothetical protein, at 1,724,706 to 1,724,957
_1626
HP15_1627: HP15_1627 - conserved hypothetical protein, at 1,725,128 to 1,725,280
_1627
HP15_1628: HP15_1628 - peptidoglycan-binding domain 1 protein-like protein, at 1,725,532 to 1,726,653
_1628
Position (kb)
1723
1724
1725 Strain fitness (log2 ratio)
-1
0
1
2 at 1722.589 kb on + strand, within HP15_1623 at 1722.589 kb on + strand, within HP15_1623 at 1722.597 kb on - strand, within HP15_1623 at 1722.606 kb on + strand, within HP15_1623 at 1722.758 kb on - strand, within HP15_1623 at 1722.834 kb on + strand, within HP15_1623 at 1722.890 kb on + strand at 1722.963 kb on + strand at 1722.963 kb on + strand at 1723.021 kb on - strand at 1723.076 kb on - strand at 1723.138 kb on + strand at 1723.202 kb on + strand, within HP15_1624 at 1723.309 kb on + strand, within HP15_1624 at 1723.376 kb on + strand at 1723.434 kb on + strand at 1723.584 kb on + strand at 1723.592 kb on - strand at 1723.664 kb on + strand at 1723.676 kb on - strand, within HP15_1625 at 1723.923 kb on - strand, within HP15_1625 at 1724.547 kb on - strand at 1724.563 kb on - strand at 1724.572 kb on + strand at 1724.576 kb on - strand at 1724.591 kb on + strand at 1724.647 kb on + strand at 1724.666 kb on + strand at 1724.671 kb on + strand at 1724.671 kb on + strand at 1724.671 kb on + strand at 1724.674 kb on - strand at 1724.674 kb on - strand at 1724.679 kb on - strand at 1724.906 kb on + strand, within HP15_1626 at 1724.914 kb on - strand, within HP15_1626 at 1724.936 kb on - strand at 1724.960 kb on - strand at 1725.010 kb on + strand at 1725.187 kb on + strand, within HP15_1627 at 1725.273 kb on - strand at 1725.473 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,722,589 + HP15_1623 0.78 +0.3 1,722,589 + HP15_1623 0.78 +1.2 1,722,597 - HP15_1623 0.79 +0.1 1,722,606 + HP15_1623 0.79 +0.7 1,722,758 - HP15_1623 0.85 -0.0 1,722,834 + HP15_1623 0.88 -0.0 1,722,890 + -0.6 1,722,963 + +0.3 1,722,963 + +0.5 1,723,021 - +0.5 1,723,076 - -0.5 1,723,138 + +0.5 1,723,202 + HP15_1624 0.23 +0.0 1,723,309 + HP15_1624 0.70 -1.3 1,723,376 + -0.2 1,723,434 + +0.1 1,723,584 + -0.8 1,723,592 - -0.7 1,723,664 + -0.2 1,723,676 - HP15_1625 0.11 -0.6 1,723,923 - HP15_1625 0.36 -1.4 1,724,547 - -0.1 1,724,563 - +2.0 1,724,572 + +0.3 1,724,576 - -0.6 1,724,591 + -1.0 1,724,647 + -0.4 1,724,666 + +0.2 1,724,671 + +0.9 1,724,671 + -0.2 1,724,671 + +0.6 1,724,674 - -0.2 1,724,674 - +0.5 1,724,679 - +0.0 1,724,906 + HP15_1626 0.79 +0.4 1,724,914 - HP15_1626 0.83 -0.7 1,724,936 - -0.8 1,724,960 - -0.5 1,725,010 + +0.2 1,725,187 + HP15_1627 0.39 +0.6 1,725,273 - -0.2 1,725,473 + -0.2
Or see this region's nucleotide sequence