Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1503 and HP15_1504 are separated by 53 nucleotides HP15_1504 and HP15_1505 are separated by 103 nucleotides HP15_1505 and HP15_1506 are separated by 145 nucleotides
HP15_1503: HP15_1503 - nucleoside-diphosphate sugar epimerase, at 1,579,904 to 1,580,830
_1503
HP15_1504: HP15_1504 - cobalamin (vitamin B12) biosynthesis CbiX protein, at 1,580,884 to 1,581,258
_1504
HP15_1505: HP15_1505 - protein containing DUF924, bacterial, at 1,581,362 to 1,581,964
_1505
HP15_1506: HP15_1506 - secreted protein containing YkuD domain, at 1,582,110 to 1,583,147
_1506
Position (kb)
1580
1581
1582 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1579.952 kb on - strand at 1579.954 kb on + strand at 1580.096 kb on + strand, within HP15_1503 at 1580.096 kb on + strand, within HP15_1503 at 1580.175 kb on - strand, within HP15_1503 at 1580.175 kb on - strand, within HP15_1503 at 1580.175 kb on - strand, within HP15_1503 at 1580.175 kb on - strand, within HP15_1503 at 1580.210 kb on + strand, within HP15_1503 at 1580.278 kb on + strand, within HP15_1503 at 1580.361 kb on - strand, within HP15_1503 at 1580.540 kb on - strand, within HP15_1503 at 1580.600 kb on + strand, within HP15_1503 at 1580.839 kb on + strand at 1580.867 kb on + strand at 1581.132 kb on + strand, within HP15_1504 at 1581.466 kb on - strand, within HP15_1505 at 1581.473 kb on - strand at 1581.665 kb on - strand, within HP15_1505 at 1581.674 kb on + strand, within HP15_1505 at 1581.739 kb on + strand, within HP15_1505 at 1581.754 kb on + strand, within HP15_1505 at 1581.798 kb on + strand, within HP15_1505 at 1581.959 kb on + strand at 1581.959 kb on - strand at 1581.969 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,579,952 - -0.6 1,579,954 + -1.3 1,580,096 + HP15_1503 0.21 -0.4 1,580,096 + HP15_1503 0.21 -0.7 1,580,175 - HP15_1503 0.29 -1.2 1,580,175 - HP15_1503 0.29 -0.0 1,580,175 - HP15_1503 0.29 -0.4 1,580,175 - HP15_1503 0.29 -0.8 1,580,210 + HP15_1503 0.33 -0.3 1,580,278 + HP15_1503 0.40 -0.4 1,580,361 - HP15_1503 0.49 -0.3 1,580,540 - HP15_1503 0.69 +0.5 1,580,600 + HP15_1503 0.75 -0.3 1,580,839 + -0.4 1,580,867 + -0.4 1,581,132 + HP15_1504 0.66 -2.9 1,581,466 - HP15_1505 0.17 -0.1 1,581,473 - +0.7 1,581,665 - HP15_1505 0.50 -0.3 1,581,674 + HP15_1505 0.52 +0.6 1,581,739 + HP15_1505 0.63 +0.5 1,581,754 + HP15_1505 0.65 -0.7 1,581,798 + HP15_1505 0.72 +0.6 1,581,959 + -1.3 1,581,959 - +0.4 1,581,969 + +0.5
Or see this region's nucleotide sequence