Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1398 and HP15_1399 are separated by 153 nucleotides HP15_1399 and HP15_1400 are separated by 30 nucleotides
HP15_1398: HP15_1398 - tRNA-dihydrouridine synthase C, at 1,474,244 to 1,475,215
_1398
HP15_1399: HP15_1399 - metal-dependent hydrolase-like protein, at 1,475,369 to 1,476,334
_1399
HP15_1400: HP15_1400 - fructose-bisphosphate aldolase B, at 1,476,365 to 1,477,429
_1400
Position (kb)
1475
1476
1477 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1474.431 kb on + strand, within HP15_1398 at 1474.506 kb on + strand, within HP15_1398 at 1474.558 kb on + strand, within HP15_1398 at 1474.566 kb on - strand, within HP15_1398 at 1474.577 kb on - strand, within HP15_1398 at 1474.582 kb on - strand, within HP15_1398 at 1474.621 kb on + strand, within HP15_1398 at 1474.719 kb on + strand, within HP15_1398 at 1474.817 kb on + strand, within HP15_1398 at 1474.866 kb on + strand, within HP15_1398 at 1474.875 kb on + strand, within HP15_1398 at 1474.883 kb on - strand, within HP15_1398 at 1474.934 kb on + strand, within HP15_1398 at 1474.989 kb on - strand, within HP15_1398 at 1475.000 kb on - strand, within HP15_1398 at 1475.051 kb on - strand, within HP15_1398 at 1475.172 kb on + strand at 1475.180 kb on - strand at 1475.251 kb on - strand at 1475.265 kb on + strand at 1475.453 kb on + strand at 1475.461 kb on - strand at 1475.461 kb on - strand at 1475.499 kb on - strand, within HP15_1399 at 1475.503 kb on - strand, within HP15_1399 at 1475.667 kb on + strand, within HP15_1399 at 1475.675 kb on - strand, within HP15_1399 at 1475.835 kb on - strand, within HP15_1399 at 1475.937 kb on - strand, within HP15_1399 at 1475.969 kb on + strand, within HP15_1399 at 1475.999 kb on + strand, within HP15_1399 at 1476.212 kb on + strand, within HP15_1399 at 1476.340 kb on + strand at 1476.438 kb on + strand at 1476.525 kb on + strand, within HP15_1400 at 1476.526 kb on + strand, within HP15_1400 at 1476.533 kb on - strand, within HP15_1400 at 1476.791 kb on + strand, within HP15_1400 at 1476.814 kb on - strand, within HP15_1400 at 1476.928 kb on - strand, within HP15_1400 at 1477.012 kb on + strand, within HP15_1400 at 1477.034 kb on + strand, within HP15_1400 at 1477.096 kb on + strand, within HP15_1400 at 1477.216 kb on + strand, within HP15_1400 at 1477.238 kb on + strand, within HP15_1400 at 1477.269 kb on + strand, within HP15_1400 at 1477.270 kb on - strand, within HP15_1400
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,474,431 + HP15_1398 0.19 -0.6 1,474,506 + HP15_1398 0.27 +0.7 1,474,558 + HP15_1398 0.32 -0.2 1,474,566 - HP15_1398 0.33 -2.0 1,474,577 - HP15_1398 0.34 -0.5 1,474,582 - HP15_1398 0.35 -0.2 1,474,621 + HP15_1398 0.39 +0.2 1,474,719 + HP15_1398 0.49 -0.4 1,474,817 + HP15_1398 0.59 +1.4 1,474,866 + HP15_1398 0.64 -0.6 1,474,875 + HP15_1398 0.65 -0.1 1,474,883 - HP15_1398 0.66 -0.2 1,474,934 + HP15_1398 0.71 +0.8 1,474,989 - HP15_1398 0.77 +0.1 1,475,000 - HP15_1398 0.78 +0.4 1,475,051 - HP15_1398 0.83 -0.3 1,475,172 + -1.3 1,475,180 - +0.1 1,475,251 - -0.8 1,475,265 + +0.1 1,475,453 + +0.8 1,475,461 - -3.0 1,475,461 - -0.7 1,475,499 - HP15_1399 0.13 -0.1 1,475,503 - HP15_1399 0.14 -1.4 1,475,667 + HP15_1399 0.31 -0.6 1,475,675 - HP15_1399 0.32 -0.4 1,475,835 - HP15_1399 0.48 -0.2 1,475,937 - HP15_1399 0.59 +0.0 1,475,969 + HP15_1399 0.62 -1.0 1,475,999 + HP15_1399 0.65 -0.3 1,476,212 + HP15_1399 0.87 -0.4 1,476,340 + +0.1 1,476,438 + +0.3 1,476,525 + HP15_1400 0.15 -0.4 1,476,526 + HP15_1400 0.15 -0.2 1,476,533 - HP15_1400 0.16 -0.6 1,476,791 + HP15_1400 0.40 +0.2 1,476,814 - HP15_1400 0.42 -0.2 1,476,928 - HP15_1400 0.53 +0.0 1,477,012 + HP15_1400 0.61 +0.6 1,477,034 + HP15_1400 0.63 +0.7 1,477,096 + HP15_1400 0.69 -0.1 1,477,216 + HP15_1400 0.80 +0.5 1,477,238 + HP15_1400 0.82 -0.1 1,477,269 + HP15_1400 0.85 -0.4 1,477,270 - HP15_1400 0.85 +1.3
Or see this region's nucleotide sequence