Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1366 and HP15_1367 are separated by 134 nucleotides HP15_1367 and HP15_1368 are separated by 59 nucleotides HP15_1368 and HP15_1369 overlap by 1 nucleotides HP15_1369 and HP15_1370 are separated by 26 nucleotides
HP15_1366: HP15_1366 - ribosomal S1 synthesis/modification protein, at 1,438,618 to 1,440,312
_1366
HP15_1367: HP15_1367 - integration host factor, beta subunit, at 1,440,447 to 1,440,743
_1367
HP15_1368: HP15_1368 - membrane protein containing DUF1049, at 1,440,803 to 1,441,105
_1368
HP15_1369: HP15_1369 - conserved hypothetical protein, at 1,441,105 to 1,442,274
_1369
HP15_1370: HP15_1370 - orotidine 5'-phosphate decarboxylase, at 1,442,301 to 1,443,017
_1370
Position (kb)
1441
1442
1443 Strain fitness (log2 ratio)
-2
-1
0
1 at 1440.776 kb on + strand at 1441.256 kb on + strand, within HP15_1369 at 1441.519 kb on + strand, within HP15_1369 at 1441.535 kb on + strand, within HP15_1369 at 1441.554 kb on + strand, within HP15_1369 at 1441.647 kb on + strand, within HP15_1369 at 1441.796 kb on + strand, within HP15_1369 at 1441.883 kb on - strand, within HP15_1369 at 1442.064 kb on + strand, within HP15_1369 at 1442.064 kb on + strand, within HP15_1369 at 1442.097 kb on + strand, within HP15_1369 at 1442.269 kb on + strand at 1442.269 kb on + strand at 1443.137 kb on + strand at 1443.139 kb on + strand at 1443.177 kb on - strand at 1443.182 kb on - strand at 1443.229 kb on + strand at 1443.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,440,776 + -0.1 1,441,256 + HP15_1369 0.13 -0.4 1,441,519 + HP15_1369 0.35 +0.2 1,441,535 + HP15_1369 0.37 -0.9 1,441,554 + HP15_1369 0.38 +0.0 1,441,647 + HP15_1369 0.46 -1.0 1,441,796 + HP15_1369 0.59 -1.2 1,441,883 - HP15_1369 0.66 -0.5 1,442,064 + HP15_1369 0.82 +0.3 1,442,064 + HP15_1369 0.82 -1.1 1,442,097 + HP15_1369 0.85 +0.0 1,442,269 + +0.4 1,442,269 + -0.3 1,443,137 + +0.3 1,443,139 + -0.7 1,443,177 - +0.3 1,443,182 - -2.3 1,443,229 + -0.3 1,443,237 - -0.4
Or see this region's nucleotide sequence