Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1289 and HP15_1290 are separated by 86 nucleotides HP15_1290 and HP15_1291 are separated by 184 nucleotides HP15_1291 and HP15_1292 are separated by 45 nucleotides
HP15_1289: HP15_1289 - electron transport complex, RnfABCDGE type, C subunit, at 1,348,616 to 1,350,358
_1289
HP15_1290: HP15_1290 - electron transport complex, RnfABCDGE type, B subunit, at 1,350,445 to 1,350,867
_1290
HP15_1291: HP15_1291 - electron transport complex, RnfA, at 1,351,052 to 1,351,633
_1291
HP15_1292: HP15_1292 - methionyl-tRNA synthetase, at 1,351,679 to 1,353,709
_1292
Position (kb)
1350
1351 Strain fitness (log2 ratio)
-2
-1
0
1 at 1349.612 kb on - strand, within HP15_1289 at 1349.717 kb on - strand, within HP15_1289 at 1349.733 kb on + strand, within HP15_1289 at 1349.814 kb on + strand, within HP15_1289 at 1349.967 kb on + strand, within HP15_1289 at 1350.116 kb on + strand, within HP15_1289 at 1350.305 kb on - strand at 1350.384 kb on + strand at 1350.392 kb on - strand at 1350.528 kb on - strand, within HP15_1290 at 1350.603 kb on - strand, within HP15_1290 at 1350.649 kb on + strand, within HP15_1290 at 1350.712 kb on - strand, within HP15_1290 at 1350.924 kb on - strand at 1350.928 kb on + strand at 1350.936 kb on - strand at 1350.936 kb on - strand at 1350.936 kb on - strand at 1350.936 kb on - strand at 1351.280 kb on + strand, within HP15_1291 at 1351.280 kb on + strand, within HP15_1291 at 1351.280 kb on + strand, within HP15_1291 at 1351.406 kb on + strand, within HP15_1291 at 1351.413 kb on + strand, within HP15_1291 at 1351.459 kb on - strand, within HP15_1291 at 1351.553 kb on - strand, within HP15_1291 at 1351.569 kb on + strand, within HP15_1291 at 1351.602 kb on + strand at 1351.684 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,349,612 - HP15_1289 0.57 -0.5 1,349,717 - HP15_1289 0.63 -0.5 1,349,733 + HP15_1289 0.64 +0.6 1,349,814 + HP15_1289 0.69 +0.3 1,349,967 + HP15_1289 0.78 -0.5 1,350,116 + HP15_1289 0.86 -0.2 1,350,305 - -0.5 1,350,384 + -0.9 1,350,392 - +0.2 1,350,528 - HP15_1290 0.20 +0.4 1,350,603 - HP15_1290 0.37 -1.7 1,350,649 + HP15_1290 0.48 -1.2 1,350,712 - HP15_1290 0.63 -0.6 1,350,924 - +0.3 1,350,928 + -0.5 1,350,936 - -0.1 1,350,936 - -0.1 1,350,936 - -0.5 1,350,936 - -1.8 1,351,280 + HP15_1291 0.39 +0.1 1,351,280 + HP15_1291 0.39 -2.0 1,351,280 + HP15_1291 0.39 -0.2 1,351,406 + HP15_1291 0.61 -1.2 1,351,413 + HP15_1291 0.62 -0.7 1,351,459 - HP15_1291 0.70 -0.3 1,351,553 - HP15_1291 0.86 -0.2 1,351,569 + HP15_1291 0.89 +0.4 1,351,602 + -0.3 1,351,684 - -0.2
Or see this region's nucleotide sequence