Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1214 and HP15_1215 are separated by 74 nucleotides HP15_1215 and HP15_1216 are separated by 74 nucleotides HP15_1216 and HP15_1217 overlap by 2 nucleotides HP15_1217 and HP15_1218 are separated by 11 nucleotides
HP15_1214: HP15_1214 - methyl-accepting chemotaxis sensory transducer, at 1,262,449 to 1,264,695
_1214
HP15_1215: HP15_1215 - ribonuclease T, at 1,264,770 to 1,265,396
_1215
HP15_1216: HP15_1216 - dihydroorotase, homodimeric type, at 1,265,471 to 1,266,502
_1216
HP15_1217: HP15_1217 - hypothetical protein, at 1,266,501 to 1,266,674
_1217
HP15_1218: HP15_1218 - OmpA/MotB domain protein, at 1,266,686 to 1,267,603
_1218
Position (kb)
1265
1266
1267 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1264.612 kb on + strand at 1264.620 kb on - strand at 1264.645 kb on + strand at 1264.789 kb on - strand at 1265.332 kb on + strand, within HP15_1215 at 1265.850 kb on + strand, within HP15_1216 at 1266.258 kb on + strand, within HP15_1216 at 1266.491 kb on - strand at 1266.717 kb on - strand at 1266.717 kb on - strand at 1266.802 kb on + strand, within HP15_1218 at 1266.869 kb on + strand, within HP15_1218 at 1266.994 kb on - strand, within HP15_1218 at 1267.112 kb on - strand, within HP15_1218 at 1267.137 kb on + strand, within HP15_1218 at 1267.241 kb on - strand, within HP15_1218 at 1267.246 kb on - strand, within HP15_1218 at 1267.246 kb on - strand, within HP15_1218 at 1267.297 kb on - strand, within HP15_1218 at 1267.302 kb on - strand, within HP15_1218 at 1267.436 kb on - strand, within HP15_1218
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,264,612 + +0.1 1,264,620 - +0.5 1,264,645 + -1.7 1,264,789 - +0.3 1,265,332 + HP15_1215 0.90 +0.3 1,265,850 + HP15_1216 0.37 -3.0 1,266,258 + HP15_1216 0.76 -1.8 1,266,491 - +0.6 1,266,717 - -0.4 1,266,717 - +0.6 1,266,802 + HP15_1218 0.13 +1.4 1,266,869 + HP15_1218 0.20 +0.6 1,266,994 - HP15_1218 0.34 +0.7 1,267,112 - HP15_1218 0.46 +0.1 1,267,137 + HP15_1218 0.49 +0.6 1,267,241 - HP15_1218 0.60 +0.1 1,267,246 - HP15_1218 0.61 +0.5 1,267,246 - HP15_1218 0.61 +0.3 1,267,297 - HP15_1218 0.67 +0.5 1,267,302 - HP15_1218 0.67 +0.3 1,267,436 - HP15_1218 0.82 +0.4
Or see this region's nucleotide sequence