Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1052 and HP15_1053 are separated by 39 nucleotides HP15_1053 and HP15_1054 are separated by 45 nucleotides HP15_1054 and HP15_1055 overlap by 4 nucleotides
HP15_1052: HP15_1052 - peptidase S16, lon domain-containing protein, at 1,072,406 to 1,074,813
_1052
HP15_1053: HP15_1053 - two component transcriptional regulator, LuxR family, at 1,074,853 to 1,075,479
_1053
HP15_1054: HP15_1054 - multi-sensor signal transduction histidine kinase, at 1,075,525 to 1,077,102
_1054
HP15_1055: HP15_1055 - MaoC domain protein, at 1,077,099 to 1,077,925
_1055
Position (kb)
1075
1076
1077
1078 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1074.530 kb on - strand, within HP15_1052 at 1074.685 kb on + strand at 1074.704 kb on + strand at 1074.709 kb on + strand at 1074.838 kb on + strand at 1075.223 kb on + strand, within HP15_1053 at 1075.231 kb on - strand, within HP15_1053 at 1075.249 kb on - strand, within HP15_1053 at 1075.387 kb on - strand, within HP15_1053 at 1075.452 kb on - strand at 1075.659 kb on + strand at 1075.730 kb on + strand, within HP15_1054 at 1075.738 kb on - strand, within HP15_1054 at 1075.757 kb on - strand, within HP15_1054 at 1076.071 kb on + strand, within HP15_1054 at 1076.204 kb on + strand, within HP15_1054 at 1076.503 kb on - strand, within HP15_1054 at 1077.043 kb on + strand at 1077.105 kb on - strand at 1077.152 kb on + strand at 1077.165 kb on - strand at 1077.364 kb on - strand, within HP15_1055 at 1077.371 kb on - strand, within HP15_1055 at 1077.661 kb on + strand, within HP15_1055 at 1077.713 kb on - strand, within HP15_1055 at 1077.795 kb on + strand, within HP15_1055 at 1077.821 kb on - strand, within HP15_1055 at 1077.984 kb on - strand at 1078.085 kb on - strand at 1078.087 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,074,530 - HP15_1052 0.88 +0.5 1,074,685 + -0.2 1,074,704 + -0.4 1,074,709 + +0.7 1,074,838 + -0.1 1,075,223 + HP15_1053 0.59 -2.8 1,075,231 - HP15_1053 0.60 -0.6 1,075,249 - HP15_1053 0.63 -1.1 1,075,387 - HP15_1053 0.85 -0.6 1,075,452 - -1.3 1,075,659 + -0.4 1,075,730 + HP15_1054 0.13 -0.9 1,075,738 - HP15_1054 0.13 -1.3 1,075,757 - HP15_1054 0.15 -0.8 1,076,071 + HP15_1054 0.35 +0.4 1,076,204 + HP15_1054 0.43 -0.5 1,076,503 - HP15_1054 0.62 -1.9 1,077,043 + -0.4 1,077,105 - -0.0 1,077,152 + -0.1 1,077,165 - +0.8 1,077,364 - HP15_1055 0.32 +0.1 1,077,371 - HP15_1055 0.33 +0.0 1,077,661 + HP15_1055 0.68 -0.7 1,077,713 - HP15_1055 0.74 +0.5 1,077,795 + HP15_1055 0.84 +0.3 1,077,821 - HP15_1055 0.87 -1.6 1,077,984 - -0.7 1,078,085 - +0.9 1,078,087 + -0.4
Or see this region's nucleotide sequence