Experiment: L-Isoleucine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1023 and HP15_1024 are separated by 90 nucleotides HP15_1024 and HP15_1025 overlap by 15 nucleotides HP15_1025 and HP15_1026 are separated by 20 nucleotides HP15_1026 and HP15_1027 are separated by 49 nucleotides
HP15_1023: HP15_1023 - 6-aminohexanoate-cyclic-dimer hydrolase, at 1,039,936 to 1,041,432
_1023
HP15_1024: HP15_1024 - hypothetical protein, at 1,041,523 to 1,041,708
_1024
HP15_1025: HP15_1025 - isoprenylcysteine carboxyl methyltransferase-like protein, at 1,041,694 to 1,042,203
_1025
HP15_1026: HP15_1026 - DnaJ-like membrane chaperone protein, at 1,042,224 to 1,043,003
_1026
HP15_1027: HP15_1027 - chemotactic transducer-related protein, at 1,043,053 to 1,045,878
_1027
Position (kb)
1041
1042
1043 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1040.731 kb on + strand, within HP15_1023 at 1040.731 kb on + strand, within HP15_1023 at 1040.739 kb on - strand, within HP15_1023 at 1040.819 kb on - strand, within HP15_1023 at 1040.823 kb on + strand, within HP15_1023 at 1040.823 kb on + strand, within HP15_1023 at 1040.860 kb on - strand, within HP15_1023 at 1040.906 kb on - strand, within HP15_1023 at 1040.970 kb on + strand, within HP15_1023 at 1041.190 kb on - strand, within HP15_1023 at 1041.195 kb on - strand, within HP15_1023 at 1041.213 kb on - strand, within HP15_1023 at 1041.314 kb on - strand at 1041.415 kb on - strand at 1041.514 kb on + strand at 1041.682 kb on - strand, within HP15_1024 at 1041.682 kb on - strand, within HP15_1024 at 1041.684 kb on + strand, within HP15_1024 at 1041.692 kb on - strand at 1041.708 kb on + strand at 1041.716 kb on - strand at 1041.763 kb on - strand, within HP15_1025 at 1041.796 kb on + strand, within HP15_1025 at 1041.978 kb on + strand, within HP15_1025 at 1042.025 kb on - strand, within HP15_1025 at 1042.214 kb on + strand at 1042.369 kb on - strand, within HP15_1026 at 1042.418 kb on - strand, within HP15_1026 at 1042.465 kb on - strand, within HP15_1026 at 1042.491 kb on + strand, within HP15_1026 at 1042.538 kb on - strand, within HP15_1026 at 1042.623 kb on + strand, within HP15_1026 at 1042.631 kb on - strand, within HP15_1026 at 1042.653 kb on + strand, within HP15_1026 at 1042.718 kb on - strand, within HP15_1026 at 1042.779 kb on - strand, within HP15_1026 at 1042.813 kb on + strand, within HP15_1026 at 1042.813 kb on + strand, within HP15_1026 at 1042.825 kb on + strand, within HP15_1026 at 1042.826 kb on - strand, within HP15_1026 at 1043.000 kb on + strand at 1043.008 kb on - strand at 1043.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine as (N) remove 1,040,731 + HP15_1023 0.53 +1.4 1,040,731 + HP15_1023 0.53 +2.4 1,040,739 - HP15_1023 0.54 +2.0 1,040,819 - HP15_1023 0.59 -0.9 1,040,823 + HP15_1023 0.59 +0.7 1,040,823 + HP15_1023 0.59 +0.1 1,040,860 - HP15_1023 0.62 +1.3 1,040,906 - HP15_1023 0.65 +0.1 1,040,970 + HP15_1023 0.69 -0.2 1,041,190 - HP15_1023 0.84 +0.0 1,041,195 - HP15_1023 0.84 -0.1 1,041,213 - HP15_1023 0.85 +0.8 1,041,314 - +0.9 1,041,415 - -0.8 1,041,514 + +0.4 1,041,682 - HP15_1024 0.85 -0.2 1,041,682 - HP15_1024 0.85 -0.4 1,041,684 + HP15_1024 0.87 +0.7 1,041,692 - -1.9 1,041,708 + +0.4 1,041,716 - -0.2 1,041,763 - HP15_1025 0.14 +0.3 1,041,796 + HP15_1025 0.20 -0.9 1,041,978 + HP15_1025 0.56 -0.4 1,042,025 - HP15_1025 0.65 -1.2 1,042,214 + -0.2 1,042,369 - HP15_1026 0.19 +0.0 1,042,418 - HP15_1026 0.25 +0.8 1,042,465 - HP15_1026 0.31 -0.2 1,042,491 + HP15_1026 0.34 -0.1 1,042,538 - HP15_1026 0.40 -0.1 1,042,623 + HP15_1026 0.51 +0.3 1,042,631 - HP15_1026 0.52 +0.6 1,042,653 + HP15_1026 0.55 -0.1 1,042,718 - HP15_1026 0.63 -0.0 1,042,779 - HP15_1026 0.71 +0.5 1,042,813 + HP15_1026 0.76 -0.3 1,042,813 + HP15_1026 0.76 +0.7 1,042,825 + HP15_1026 0.77 -1.5 1,042,826 - HP15_1026 0.77 -0.3 1,043,000 + -0.0 1,043,008 - +0.5 1,043,181 - -0.6
Or see this region's nucleotide sequence