Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2332 and Dsui_2333 are separated by 10 nucleotides Dsui_2333 and Dsui_2334 are separated by 151 nucleotides Dsui_2334 and Dsui_2335 are separated by 29 nucleotides Dsui_2335 and Dsui_2336 overlap by 4 nucleotides
Dsui_2332: Dsui_2332 - uracil-xanthine permease, at 2,485,682 to 2,486,923
_2332
Dsui_2333: Dsui_2333 - uracil phosphoribosyltransferase, at 2,486,934 to 2,487,560
_2333
Dsui_2334: Dsui_2334 - tRNA-dihydrouridine synthase, at 2,487,712 to 2,488,674
_2334
Dsui_2335: Dsui_2335 - Integral membrane protein CcmA involved in cell shape determination, at 2,488,704 to 2,489,153
_2335
Dsui_2336: Dsui_2336 - hypothetical protein, at 2,489,150 to 2,489,743
_2336
Position (kb)
2487
2488
2489 Strain fitness (log2 ratio)
-1
0
1
2 at 2486.801 kb on - strand at 2486.803 kb on + strand at 2486.811 kb on - strand at 2486.903 kb on - strand at 2486.908 kb on - strand at 2486.975 kb on - strand at 2486.983 kb on + strand at 2486.983 kb on + strand at 2486.983 kb on + strand at 2486.991 kb on - strand at 2486.991 kb on - strand at 2487.076 kb on - strand, within Dsui_2333 at 2487.112 kb on + strand at 2487.197 kb on + strand, within Dsui_2333 at 2487.229 kb on + strand at 2487.248 kb on - strand, within Dsui_2333 at 2487.259 kb on + strand at 2487.450 kb on - strand, within Dsui_2333 at 2487.620 kb on - strand at 2487.749 kb on - strand at 2488.015 kb on + strand, within Dsui_2334 at 2488.040 kb on + strand, within Dsui_2334 at 2488.040 kb on + strand, within Dsui_2334 at 2488.076 kb on - strand, within Dsui_2334 at 2488.124 kb on + strand, within Dsui_2334 at 2488.260 kb on + strand, within Dsui_2334 at 2488.362 kb on - strand, within Dsui_2334 at 2488.461 kb on + strand, within Dsui_2334 at 2488.521 kb on - strand, within Dsui_2334 at 2488.539 kb on + strand, within Dsui_2334 at 2488.606 kb on + strand at 2488.730 kb on + strand at 2488.742 kb on - strand at 2488.752 kb on - strand, within Dsui_2335 at 2488.849 kb on + strand at 2488.849 kb on + strand, within Dsui_2335 at 2488.849 kb on + strand, within Dsui_2335 at 2488.857 kb on - strand, within Dsui_2335 at 2488.857 kb on - strand, within Dsui_2335 at 2488.857 kb on - strand, within Dsui_2335 at 2488.914 kb on + strand, within Dsui_2335 at 2488.915 kb on + strand at 2489.033 kb on + strand, within Dsui_2335 at 2489.061 kb on + strand, within Dsui_2335 at 2489.067 kb on - strand, within Dsui_2335 at 2489.077 kb on - strand, within Dsui_2335 at 2489.094 kb on + strand, within Dsui_2335 at 2489.102 kb on - strand, within Dsui_2335 at 2489.130 kb on + strand at 2489.230 kb on + strand, within Dsui_2336 at 2489.238 kb on - strand, within Dsui_2336 at 2489.238 kb on - strand, within Dsui_2336 at 2489.238 kb on - strand, within Dsui_2336 at 2489.280 kb on - strand, within Dsui_2336 at 2489.285 kb on - strand, within Dsui_2336 at 2489.411 kb on - strand, within Dsui_2336 at 2489.443 kb on - strand, within Dsui_2336 at 2489.469 kb on - strand, within Dsui_2336 at 2489.469 kb on - strand, within Dsui_2336 at 2489.469 kb on - strand, within Dsui_2336 at 2489.469 kb on - strand, within Dsui_2336 at 2489.554 kb on + strand, within Dsui_2336 at 2489.648 kb on + strand, within Dsui_2336
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 2,486,801 - +0.6 2,486,803 + +1.2 2,486,811 - -0.3 2,486,903 - +0.4 2,486,908 - +0.3 2,486,975 - +0.0 2,486,983 + +0.6 2,486,983 + +0.4 2,486,983 + +1.2 2,486,991 - +0.2 2,486,991 - +0.6 2,487,076 - Dsui_2333 0.23 +0.0 2,487,112 + +1.6 2,487,197 + Dsui_2333 0.42 -0.1 2,487,229 + +1.6 2,487,248 - Dsui_2333 0.50 +0.9 2,487,259 + +0.2 2,487,450 - Dsui_2333 0.82 +0.4 2,487,620 - +0.2 2,487,749 - +0.6 2,488,015 + Dsui_2334 0.31 -1.6 2,488,040 + Dsui_2334 0.34 +1.9 2,488,040 + Dsui_2334 0.34 -0.1 2,488,076 - Dsui_2334 0.38 -0.1 2,488,124 + Dsui_2334 0.43 +0.4 2,488,260 + Dsui_2334 0.57 -1.0 2,488,362 - Dsui_2334 0.67 -0.7 2,488,461 + Dsui_2334 0.78 -0.2 2,488,521 - Dsui_2334 0.84 -0.9 2,488,539 + Dsui_2334 0.86 +1.3 2,488,606 + -0.1 2,488,730 + +0.6 2,488,742 - +0.5 2,488,752 - Dsui_2335 0.11 +0.1 2,488,849 + +2.2 2,488,849 + Dsui_2335 0.32 -0.4 2,488,849 + Dsui_2335 0.32 +0.8 2,488,857 - Dsui_2335 0.34 -0.5 2,488,857 - Dsui_2335 0.34 +0.6 2,488,857 - Dsui_2335 0.34 +0.2 2,488,914 + Dsui_2335 0.47 +0.6 2,488,915 + +2.2 2,489,033 + Dsui_2335 0.73 +0.8 2,489,061 + Dsui_2335 0.79 +2.2 2,489,067 - Dsui_2335 0.81 +1.9 2,489,077 - Dsui_2335 0.83 +0.6 2,489,094 + Dsui_2335 0.87 +0.0 2,489,102 - Dsui_2335 0.88 -0.8 2,489,130 + +2.2 2,489,230 + Dsui_2336 0.13 +0.3 2,489,238 - Dsui_2336 0.15 -0.2 2,489,238 - Dsui_2336 0.15 +0.8 2,489,238 - Dsui_2336 0.15 +1.5 2,489,280 - Dsui_2336 0.22 +1.1 2,489,285 - Dsui_2336 0.23 -0.7 2,489,411 - Dsui_2336 0.44 +0.1 2,489,443 - Dsui_2336 0.49 +0.1 2,489,469 - Dsui_2336 0.54 -0.2 2,489,469 - Dsui_2336 0.54 -0.1 2,489,469 - Dsui_2336 0.54 +0.8 2,489,469 - Dsui_2336 0.54 -0.2 2,489,554 + Dsui_2336 0.68 +0.1 2,489,648 + Dsui_2336 0.84 +0.6
Or see this region's nucleotide sequence