Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1310 and Dsui_1311 are separated by 85 nucleotides Dsui_1311 and Dsui_1312 are separated by 168 nucleotides Dsui_1312 and Dsui_1313 overlap by 4 nucleotides Dsui_1313 and Dsui_1314 are separated by 3 nucleotides Dsui_1314 and Dsui_1315 are separated by 15 nucleotides
Dsui_1310: Dsui_1310 - molecular chaperone (small heat shock protein), at 1,371,222 to 1,371,653
_1310
Dsui_1311: Dsui_1311 - hypothetical protein, at 1,371,739 to 1,372,083
_1311
Dsui_1312: Dsui_1312 - NTP pyrophosphohydrolase, at 1,372,252 to 1,372,719
_1312
Dsui_1313: Dsui_1313 - Barstar, RNAse (barnase) inhibitor, at 1,372,716 to 1,373,162
_1313
Dsui_1314: Dsui_1314 - guanyl-specific ribonuclease Sa, at 1,373,166 to 1,373,558
_1314
Dsui_1315: Dsui_1315 - aspartyl-tRNA synthetase, at 1,373,574 to 1,375,373
_1315
Position (kb)
1372
1373 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1371.267 kb on - strand, within Dsui_1310 at 1371.403 kb on + strand, within Dsui_1310 at 1371.413 kb on + strand, within Dsui_1310 at 1371.452 kb on - strand, within Dsui_1310 at 1371.493 kb on + strand, within Dsui_1310 at 1371.619 kb on - strand at 1371.724 kb on - strand at 1371.729 kb on - strand at 1371.751 kb on + strand at 1371.781 kb on - strand, within Dsui_1311 at 1371.800 kb on + strand at 1371.812 kb on - strand, within Dsui_1311 at 1371.824 kb on - strand, within Dsui_1311 at 1371.829 kb on + strand at 1371.980 kb on - strand, within Dsui_1311 at 1372.076 kb on - strand at 1372.206 kb on - strand at 1372.314 kb on + strand at 1372.356 kb on + strand at 1372.356 kb on + strand at 1372.360 kb on - strand, within Dsui_1312 at 1372.402 kb on - strand, within Dsui_1312 at 1372.422 kb on + strand at 1372.489 kb on - strand, within Dsui_1312 at 1372.492 kb on + strand, within Dsui_1312 at 1372.647 kb on + strand, within Dsui_1312 at 1372.649 kb on + strand, within Dsui_1312 at 1372.649 kb on - strand, within Dsui_1312 at 1372.789 kb on - strand, within Dsui_1313 at 1372.824 kb on + strand at 1372.837 kb on - strand, within Dsui_1313 at 1372.837 kb on - strand, within Dsui_1313 at 1372.856 kb on - strand, within Dsui_1313 at 1372.879 kb on + strand at 1372.896 kb on + strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.909 kb on - strand, within Dsui_1313 at 1373.110 kb on + strand, within Dsui_1313 at 1373.110 kb on + strand at 1373.118 kb on - strand at 1373.298 kb on + strand at 1373.305 kb on + strand, within Dsui_1314 at 1373.320 kb on + strand at 1373.375 kb on - strand, within Dsui_1314 at 1373.393 kb on + strand, within Dsui_1314 at 1373.394 kb on + strand at 1373.571 kb on + strand at 1373.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 1,371,267 - Dsui_1310 0.10 -0.4 1,371,403 + Dsui_1310 0.42 -0.4 1,371,413 + Dsui_1310 0.44 -0.8 1,371,452 - Dsui_1310 0.53 +0.1 1,371,493 + Dsui_1310 0.63 -0.8 1,371,619 - +0.2 1,371,724 - +0.6 1,371,729 - -0.9 1,371,751 + -0.3 1,371,781 - Dsui_1311 0.12 -0.7 1,371,800 + -0.8 1,371,812 - Dsui_1311 0.21 -0.2 1,371,824 - Dsui_1311 0.25 -0.2 1,371,829 + -0.6 1,371,980 - Dsui_1311 0.70 -0.0 1,372,076 - +0.2 1,372,206 - -0.2 1,372,314 + +3.2 1,372,356 + +0.9 1,372,356 + +1.6 1,372,360 - Dsui_1312 0.23 -0.6 1,372,402 - Dsui_1312 0.32 -0.8 1,372,422 + +1.2 1,372,489 - Dsui_1312 0.51 -0.7 1,372,492 + Dsui_1312 0.51 -0.3 1,372,647 + Dsui_1312 0.84 -0.6 1,372,649 + Dsui_1312 0.85 -0.7 1,372,649 - Dsui_1312 0.85 -0.4 1,372,789 - Dsui_1313 0.16 -0.1 1,372,824 + +1.2 1,372,837 - Dsui_1313 0.27 -0.1 1,372,837 - Dsui_1313 0.27 -1.9 1,372,856 - Dsui_1313 0.31 +0.2 1,372,879 + +2.2 1,372,896 + Dsui_1313 0.40 +0.6 1,372,904 - Dsui_1313 0.42 -1.0 1,372,904 - Dsui_1313 0.42 -0.5 1,372,904 - Dsui_1313 0.42 -0.7 1,372,904 - Dsui_1313 0.42 +1.6 1,372,909 - Dsui_1313 0.43 -1.8 1,373,110 + Dsui_1313 0.88 +0.9 1,373,110 + +1.6 1,373,118 - +0.1 1,373,298 + +3.2 1,373,305 + Dsui_1314 0.35 -0.1 1,373,320 + +2.2 1,373,375 - Dsui_1314 0.53 +0.7 1,373,393 + Dsui_1314 0.58 -0.4 1,373,394 + -0.1 1,373,571 + -0.7 1,373,579 - +0.1
Or see this region's nucleotide sequence