Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0990 and Dsui_0991 overlap by 14 nucleotides Dsui_0991 and Dsui_0992 overlap by 20 nucleotides Dsui_0992 and Dsui_0993 overlap by 8 nucleotides Dsui_0993 and Dsui_0994 overlap by 1 nucleotides
Dsui_0990: Dsui_0990 - putative P-loop-containing kinase, at 1,017,863 to 1,018,735
_0990
Dsui_0991: Dsui_0991 - hypothetical protein, at 1,018,722 to 1,019,150
_0991
Dsui_0992: Dsui_0992 - putative membrane protein, at 1,019,131 to 1,019,706
_0992
Dsui_0993: Dsui_0993 - polyprenyl p-hydroxybenzoate/phenylacrylic acid decarboxylase, at 1,019,699 to 1,020,316
_0993
Dsui_0994: Dsui_0994 - peptidase S16, lon domain protein, at 1,020,316 to 1,020,957
_0994
Position (kb)
1019
1020 Strain fitness (log2 ratio)
-2
-1
0
1 at 1018.374 kb on + strand, within Dsui_0990 at 1018.764 kb on + strand at 1018.824 kb on - strand at 1018.935 kb on - strand at 1019.036 kb on - strand, within Dsui_0991 at 1019.279 kb on + strand, within Dsui_0992 at 1019.433 kb on + strand, within Dsui_0992 at 1019.488 kb on - strand at 1019.701 kb on + strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1020.458 kb on - strand, within Dsui_0994 at 1020.463 kb on - strand at 1020.500 kb on - strand, within Dsui_0994 at 1020.514 kb on + strand, within Dsui_0994
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 1,018,374 + Dsui_0990 0.59 +0.6 1,018,764 + +0.7 1,018,824 - +0.6 1,018,935 - +1.2 1,019,036 - Dsui_0991 0.73 -0.6 1,019,279 + Dsui_0992 0.26 -1.3 1,019,433 + Dsui_0992 0.52 -2.1 1,019,488 - +0.6 1,019,701 + -0.1 1,019,709 - -0.2 1,019,709 - -0.4 1,019,709 - +0.0 1,019,709 - -0.2 1,020,458 - Dsui_0994 0.22 -0.4 1,020,463 - -0.4 1,020,500 - Dsui_0994 0.29 +0.2 1,020,514 + Dsui_0994 0.31 -1.1
Or see this region's nucleotide sequence