Experiment: Uridine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_2095 and upp are separated by 88 nucleotides upp and Sama_2097 are separated by 232 nucleotides Sama_2097 and purN overlap by 1 nucleotides purN and Sama_2099 are separated by 254 nucleotides
Sama_2095: Sama_2095 - hypothetical protein (RefSeq), at 2,539,113 to 2,540,435
_2095
Sama_2096: upp - uracil phosphoribosyltransferase (RefSeq), at 2,540,524 to 2,541,150
upp
Sama_2097: Sama_2097 - phosphoribosylaminoimidazole synthetase (RefSeq), at 2,541,383 to 2,542,420
_2097
Sama_2098: purN - phosphoribosylglycinamide formyltransferase (RefSeq), at 2,542,420 to 2,543,058
purN
Sama_2099: Sama_2099 - UMP phosphatase (EC 3.1.3.5) (from data) , at 2,543,313 to 2,544,059
_2099
Position (kb)
2541
2542
2543 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2540.385 kb on + strand at 2540.385 kb on + strand at 2540.431 kb on + strand at 2540.431 kb on + strand at 2540.431 kb on + strand at 2540.431 kb on + strand at 2540.432 kb on - strand at 2540.432 kb on - strand at 2540.432 kb on - strand at 2540.445 kb on + strand at 2540.445 kb on + strand at 2540.450 kb on + strand at 2540.450 kb on + strand at 2540.459 kb on + strand at 2540.460 kb on - strand at 2540.516 kb on - strand at 2540.516 kb on - strand at 2540.526 kb on - strand at 2540.526 kb on - strand at 2540.526 kb on - strand at 2540.526 kb on - strand at 2540.615 kb on + strand, within upp at 2540.698 kb on - strand, within upp at 2540.698 kb on - strand, within upp at 2540.847 kb on - strand, within upp at 2540.847 kb on - strand, within upp at 2540.851 kb on - strand, within upp at 2540.854 kb on + strand, within upp at 2540.854 kb on + strand, within upp at 2540.855 kb on - strand, within upp at 2540.855 kb on - strand, within upp at 2540.855 kb on - strand, within upp at 2540.880 kb on + strand, within upp at 2540.926 kb on - strand, within upp at 2540.926 kb on - strand, within upp at 2540.932 kb on + strand, within upp at 2540.932 kb on + strand, within upp at 2540.933 kb on - strand, within upp at 2540.933 kb on - strand, within upp at 2541.011 kb on - strand, within upp at 2541.011 kb on - strand, within upp at 2541.029 kb on + strand, within upp at 2541.030 kb on - strand, within upp at 2541.030 kb on - strand, within upp at 2541.030 kb on - strand, within upp at 2541.155 kb on - strand at 2541.155 kb on - strand at 2541.155 kb on - strand at 2541.155 kb on - strand at 2541.155 kb on - strand at 2541.330 kb on + strand at 2541.330 kb on + strand at 2541.362 kb on + strand at 2541.362 kb on + strand at 2541.367 kb on + strand at 2541.367 kb on + strand at 2541.367 kb on + strand at 2541.368 kb on - strand at 2541.368 kb on - strand at 2541.368 kb on - strand at 2541.370 kb on + strand at 2541.370 kb on + strand at 2541.370 kb on + strand at 2541.371 kb on - strand at 2541.407 kb on + strand at 2541.407 kb on + strand at 2541.408 kb on - strand at 2541.408 kb on - strand at 2541.410 kb on - strand at 2541.410 kb on - strand at 2541.410 kb on - strand at 2541.545 kb on + strand, within Sama_2097 at 2541.545 kb on + strand, within Sama_2097 at 2541.545 kb on + strand, within Sama_2097 at 2541.545 kb on + strand, within Sama_2097 at 2541.545 kb on + strand, within Sama_2097 at 2541.545 kb on + strand, within Sama_2097 at 2541.546 kb on - strand, within Sama_2097 at 2541.634 kb on + strand, within Sama_2097 at 2541.705 kb on - strand, within Sama_2097 at 2541.705 kb on - strand, within Sama_2097 at 2541.707 kb on + strand, within Sama_2097 at 2541.707 kb on + strand, within Sama_2097 at 2541.708 kb on - strand, within Sama_2097 at 2541.708 kb on - strand, within Sama_2097 at 2541.729 kb on + strand, within Sama_2097 at 2541.730 kb on - strand, within Sama_2097 at 2541.748 kb on - strand, within Sama_2097 at 2541.750 kb on + strand, within Sama_2097 at 2541.750 kb on + strand, within Sama_2097 at 2541.827 kb on + strand, within Sama_2097 at 2541.827 kb on + strand, within Sama_2097 at 2541.827 kb on + strand, within Sama_2097 at 2541.827 kb on + strand, within Sama_2097 at 2541.827 kb on + strand, within Sama_2097 at 2541.842 kb on + strand, within Sama_2097 at 2541.864 kb on + strand, within Sama_2097 at 2541.906 kb on + strand, within Sama_2097 at 2541.981 kb on + strand, within Sama_2097 at 2541.981 kb on + strand, within Sama_2097 at 2542.064 kb on + strand, within Sama_2097 at 2542.104 kb on + strand, within Sama_2097 at 2542.253 kb on + strand, within Sama_2097 at 2542.262 kb on + strand, within Sama_2097 at 2542.263 kb on - strand, within Sama_2097 at 2542.339 kb on + strand at 2542.387 kb on + strand at 2542.443 kb on + strand at 2542.443 kb on + strand at 2542.443 kb on + strand at 2542.443 kb on + strand at 2542.443 kb on + strand at 2542.530 kb on + strand, within purN at 2542.530 kb on + strand, within purN at 2542.543 kb on + strand, within purN at 2542.543 kb on + strand, within purN at 2542.543 kb on + strand, within purN at 2542.543 kb on + strand, within purN at 2542.543 kb on + strand, within purN at 2542.544 kb on - strand, within purN at 2542.574 kb on + strand, within purN at 2542.621 kb on + strand, within purN at 2542.622 kb on - strand, within purN at 2542.654 kb on + strand, within purN at 2542.654 kb on + strand, within purN at 2542.655 kb on - strand, within purN at 2542.686 kb on + strand, within purN at 2542.686 kb on + strand, within purN at 2542.740 kb on + strand, within purN at 2542.740 kb on + strand, within purN at 2542.740 kb on + strand, within purN at 2542.765 kb on + strand, within purN at 2542.883 kb on + strand, within purN at 2542.891 kb on + strand, within purN at 2542.891 kb on + strand, within purN at 2542.891 kb on + strand, within purN at 2542.891 kb on + strand, within purN at 2542.891 kb on + strand, within purN at 2542.928 kb on + strand, within purN at 2542.951 kb on + strand, within purN at 2543.012 kb on - strand at 2543.141 kb on - strand at 2543.193 kb on - strand at 2543.213 kb on - strand at 2543.226 kb on - strand at 2543.254 kb on + strand at 2543.254 kb on + strand at 2543.255 kb on - strand at 2543.259 kb on - strand at 2543.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine remove 2,540,385 + -0.6 2,540,385 + -0.8 2,540,431 + -0.1 2,540,431 + +1.1 2,540,431 + +0.7 2,540,431 + -0.1 2,540,432 - +0.3 2,540,432 - +1.0 2,540,432 - +2.5 2,540,445 + -0.2 2,540,445 + -0.3 2,540,450 + +0.0 2,540,450 + +0.1 2,540,459 + +0.0 2,540,460 - -0.8 2,540,516 - -0.2 2,540,516 - -2.6 2,540,526 - -0.0 2,540,526 - +1.1 2,540,526 - +0.5 2,540,526 - +0.1 2,540,615 + upp Sama_2096 0.15 -0.1 2,540,698 - upp Sama_2096 0.28 -0.0 2,540,698 - upp Sama_2096 0.28 -0.5 2,540,847 - upp Sama_2096 0.52 +0.7 2,540,847 - upp Sama_2096 0.52 +0.9 2,540,851 - upp Sama_2096 0.52 +0.1 2,540,854 + upp Sama_2096 0.53 +1.0 2,540,854 + upp Sama_2096 0.53 -0.2 2,540,855 - upp Sama_2096 0.53 -0.6 2,540,855 - upp Sama_2096 0.53 -0.2 2,540,855 - upp Sama_2096 0.53 +0.5 2,540,880 + upp Sama_2096 0.57 +0.1 2,540,926 - upp Sama_2096 0.64 -0.8 2,540,926 - upp Sama_2096 0.64 +0.1 2,540,932 + upp Sama_2096 0.65 +0.4 2,540,932 + upp Sama_2096 0.65 -0.3 2,540,933 - upp Sama_2096 0.65 +0.5 2,540,933 - upp Sama_2096 0.65 +0.2 2,541,011 - upp Sama_2096 0.78 -0.3 2,541,011 - upp Sama_2096 0.78 -0.2 2,541,029 + upp Sama_2096 0.81 +0.2 2,541,030 - upp Sama_2096 0.81 -0.6 2,541,030 - upp Sama_2096 0.81 -0.2 2,541,030 - upp Sama_2096 0.81 -0.6 2,541,155 - +0.2 2,541,155 - -0.3 2,541,155 - -1.6 2,541,155 - +0.4 2,541,155 - +0.5 2,541,330 + -1.8 2,541,330 + +0.6 2,541,362 + +0.5 2,541,362 + +0.1 2,541,367 + -0.6 2,541,367 + +0.2 2,541,367 + +1.2 2,541,368 - -2.0 2,541,368 - -2.3 2,541,368 - -2.2 2,541,370 + -0.3 2,541,370 + +1.9 2,541,370 + +0.5 2,541,371 - -2.3 2,541,407 + -3.8 2,541,407 + -2.8 2,541,408 - -2.6 2,541,408 - -2.3 2,541,410 - -4.2 2,541,410 - -2.9 2,541,410 - -3.0 2,541,545 + Sama_2097 0.16 -2.9 2,541,545 + Sama_2097 0.16 -1.0 2,541,545 + Sama_2097 0.16 -3.9 2,541,545 + Sama_2097 0.16 -3.5 2,541,545 + Sama_2097 0.16 -1.8 2,541,545 + Sama_2097 0.16 -2.6 2,541,546 - Sama_2097 0.16 -3.2 2,541,634 + Sama_2097 0.24 -2.0 2,541,705 - Sama_2097 0.31 -2.0 2,541,705 - Sama_2097 0.31 -2.6 2,541,707 + Sama_2097 0.31 -2.0 2,541,707 + Sama_2097 0.31 -1.5 2,541,708 - Sama_2097 0.31 -2.9 2,541,708 - Sama_2097 0.31 -2.5 2,541,729 + Sama_2097 0.33 -2.0 2,541,730 - Sama_2097 0.33 -3.6 2,541,748 - Sama_2097 0.35 -3.5 2,541,750 + Sama_2097 0.35 -2.0 2,541,750 + Sama_2097 0.35 -1.0 2,541,827 + Sama_2097 0.43 -2.3 2,541,827 + Sama_2097 0.43 -3.2 2,541,827 + Sama_2097 0.43 -3.5 2,541,827 + Sama_2097 0.43 -3.5 2,541,827 + Sama_2097 0.43 -4.0 2,541,842 + Sama_2097 0.44 -3.2 2,541,864 + Sama_2097 0.46 -3.2 2,541,906 + Sama_2097 0.50 -3.0 2,541,981 + Sama_2097 0.58 -2.5 2,541,981 + Sama_2097 0.58 -2.6 2,542,064 + Sama_2097 0.66 -3.3 2,542,104 + Sama_2097 0.69 -3.0 2,542,253 + Sama_2097 0.84 -4.0 2,542,262 + Sama_2097 0.85 -1.8 2,542,263 - Sama_2097 0.85 -1.0 2,542,339 + -2.3 2,542,387 + -1.5 2,542,443 + -1.8 2,542,443 + -1.5 2,542,443 + -1.5 2,542,443 + -4.0 2,542,443 + -2.8 2,542,530 + purN Sama_2098 0.17 -1.5 2,542,530 + purN Sama_2098 0.17 -3.6 2,542,543 + purN Sama_2098 0.19 -2.6 2,542,543 + purN Sama_2098 0.19 -1.3 2,542,543 + purN Sama_2098 0.19 -0.2 2,542,543 + purN Sama_2098 0.19 -2.0 2,542,543 + purN Sama_2098 0.19 -1.5 2,542,544 - purN Sama_2098 0.19 -1.0 2,542,574 + purN Sama_2098 0.24 -3.5 2,542,621 + purN Sama_2098 0.31 -1.8 2,542,622 - purN Sama_2098 0.32 -3.3 2,542,654 + purN Sama_2098 0.37 -2.9 2,542,654 + purN Sama_2098 0.37 -1.3 2,542,655 - purN Sama_2098 0.37 -2.3 2,542,686 + purN Sama_2098 0.42 -2.5 2,542,686 + purN Sama_2098 0.42 -3.0 2,542,740 + purN Sama_2098 0.50 -2.2 2,542,740 + purN Sama_2098 0.50 -1.5 2,542,740 + purN Sama_2098 0.50 -1.0 2,542,765 + purN Sama_2098 0.54 -1.8 2,542,883 + purN Sama_2098 0.72 -3.3 2,542,891 + purN Sama_2098 0.74 -1.3 2,542,891 + purN Sama_2098 0.74 -1.0 2,542,891 + purN Sama_2098 0.74 -1.0 2,542,891 + purN Sama_2098 0.74 -1.8 2,542,891 + purN Sama_2098 0.74 -2.3 2,542,928 + purN Sama_2098 0.79 -2.8 2,542,951 + purN Sama_2098 0.83 -2.0 2,543,012 - -1.0 2,543,141 - -0.5 2,543,193 - -0.6 2,543,213 - +1.5 2,543,226 - +0.5 2,543,254 + +0.4 2,543,254 + +0.1 2,543,255 - -1.3 2,543,259 - -0.3 2,543,345 - -0.5
Or see this region's nucleotide sequence