Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2962 and Dsui_2963 overlap by 8 nucleotides Dsui_2963 and Dsui_2964 are separated by 3 nucleotides Dsui_2964 and Dsui_2965 are separated by 28 nucleotides
Dsui_2962: Dsui_2962 - cobalamin biosynthesis protein CbiD, at 3,169,727 to 3,170,923
_2962
Dsui_2963: Dsui_2963 - precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit,precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit, at 3,170,916 to 3,172,208
_2963
Dsui_2964: Dsui_2964 - precorrin-2 C20-methyltransferase, at 3,172,212 to 3,173,012
_2964
Dsui_2965: Dsui_2965 - cobalamin biosynthesis protein CbiG, at 3,173,041 to 3,173,826
_2965
Position (kb)
3170
3171
3172
3173 Strain fitness (log2 ratio)
-1
0
1
2 at 3169.943 kb on - strand, within Dsui_2962 at 3170.159 kb on - strand at 3170.250 kb on + strand, within Dsui_2962 at 3170.250 kb on + strand, within Dsui_2962 at 3170.250 kb on + strand, within Dsui_2962 at 3170.258 kb on - strand at 3170.258 kb on - strand at 3170.258 kb on - strand at 3170.365 kb on + strand, within Dsui_2962 at 3170.429 kb on + strand, within Dsui_2962 at 3170.444 kb on + strand, within Dsui_2962 at 3170.533 kb on + strand, within Dsui_2962 at 3170.578 kb on + strand, within Dsui_2962 at 3170.598 kb on + strand, within Dsui_2962 at 3170.626 kb on - strand at 3170.643 kb on + strand, within Dsui_2962 at 3170.930 kb on + strand at 3170.938 kb on - strand at 3170.938 kb on - strand at 3171.217 kb on - strand at 3171.324 kb on - strand at 3171.566 kb on - strand, within Dsui_2963 at 3171.580 kb on + strand, within Dsui_2963 at 3171.737 kb on + strand, within Dsui_2963 at 3171.806 kb on + strand, within Dsui_2963 at 3171.806 kb on + strand, within Dsui_2963 at 3171.814 kb on - strand, within Dsui_2963 at 3172.068 kb on + strand, within Dsui_2963 at 3172.129 kb on + strand at 3172.210 kb on - strand at 3172.359 kb on - strand, within Dsui_2964 at 3172.381 kb on + strand, within Dsui_2964 at 3172.498 kb on + strand, within Dsui_2964 at 3172.603 kb on - strand at 3172.668 kb on + strand, within Dsui_2964 at 3172.808 kb on + strand, within Dsui_2964 at 3172.914 kb on - strand, within Dsui_2964 at 3173.016 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 3,169,943 - Dsui_2962 0.18 +0.6 3,170,159 - +2.2 3,170,250 + Dsui_2962 0.44 -0.8 3,170,250 + Dsui_2962 0.44 +0.1 3,170,250 + Dsui_2962 0.44 -0.8 3,170,258 - -0.2 3,170,258 - -0.8 3,170,258 - +1.2 3,170,365 + Dsui_2962 0.53 -0.2 3,170,429 + Dsui_2962 0.59 -0.5 3,170,444 + Dsui_2962 0.60 -1.3 3,170,533 + Dsui_2962 0.67 +0.6 3,170,578 + Dsui_2962 0.71 -0.1 3,170,598 + Dsui_2962 0.73 +0.4 3,170,626 - +0.6 3,170,643 + Dsui_2962 0.77 -0.0 3,170,930 + -1.4 3,170,938 - +1.2 3,170,938 - +2.2 3,171,217 - +2.2 3,171,324 - +0.2 3,171,566 - Dsui_2963 0.50 -0.8 3,171,580 + Dsui_2963 0.51 -1.3 3,171,737 + Dsui_2963 0.63 -0.5 3,171,806 + Dsui_2963 0.69 +0.3 3,171,806 + Dsui_2963 0.69 -1.2 3,171,814 - Dsui_2963 0.69 -0.2 3,172,068 + Dsui_2963 0.89 -0.7 3,172,129 + +0.8 3,172,210 - +0.2 3,172,359 - Dsui_2964 0.18 +0.3 3,172,381 + Dsui_2964 0.21 -1.0 3,172,498 + Dsui_2964 0.36 -0.2 3,172,603 - -0.4 3,172,668 + Dsui_2964 0.57 -0.0 3,172,808 + Dsui_2964 0.74 -0.1 3,172,914 - Dsui_2964 0.88 -0.5 3,173,016 + +0.9
Or see this region's nucleotide sequence