Experiment: MoYLS4 with Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2494 and DvMF_2495 are separated by 223 nucleotides DvMF_2495 and DvMF_2496 are separated by 116 nucleotides DvMF_2496 and DvMF_2497 are separated by 6 nucleotides DvMF_2497 and DvMF_2498 are separated by 547 nucleotides
DvMF_2494: DvMF_2494 - nucleotide sugar dehydrogenase (RefSeq), at 3,146,629 to 3,147,945
_2494
DvMF_2495: DvMF_2495 - hypothetical protein (RefSeq), at 3,148,169 to 3,148,375
_2495
DvMF_2496: DvMF_2496 - ubiquinone/menaquinone biosynthesis methyltransferase (RefSeq), at 3,148,492 to 3,149,670
_2496
DvMF_2497: DvMF_2497 - hypothetical protein (RefSeq), at 3,149,677 to 3,149,883
_2497
DvMF_2498: DvMF_2498 - hypothetical protein (RefSeq), at 3,150,431 to 3,151,990
_2498
Position (kb)
3148
3149
3150 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3147.622 kb on + strand, within DvMF_2494 at 3147.651 kb on - strand, within DvMF_2494 at 3147.656 kb on - strand, within DvMF_2494 at 3148.187 kb on + strand at 3148.187 kb on + strand at 3148.562 kb on + strand at 3148.589 kb on - strand at 3149.891 kb on + strand at 3150.287 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.0125 mg/ml remove 3,147,622 + DvMF_2494 0.75 -2.2 3,147,651 - DvMF_2494 0.78 -3.4 3,147,656 - DvMF_2494 0.78 -1.4 3,148,187 + +0.0 3,148,187 + -0.4 3,148,562 + -0.3 3,148,589 - -0.2 3,149,891 + +0.4 3,150,287 + -0.3
Or see this region's nucleotide sequence