Strain Fitness in Shewanella oneidensis MR-1 around SO3436

Experiment: perchlorate 50 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpcm and surE overlap by 4 nucleotidessurE and SO3436 overlap by 1 nucleotidesSO3436 and ispF are separated by 28 nucleotidesispF and ispD are separated by 52 nucleotides SO3434: pcm - protein-L-isoaspartate O-methyltransferase (NCBI ptt file), at 3,579,274 to 3,579,909 pcm SO3435: surE - stationary-phase survival protein SurE (NCBI ptt file), at 3,579,906 to 3,580,652 surE SO3436: SO3436 - conserved hypothetical protein (NCBI ptt file), at 3,580,652 to 3,581,764 SO3436 SO3437: ispF - 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (NCBI ptt file), at 3,581,793 to 3,582,272 ispF SO3438: ispD - 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (NCBI ptt file), at 3,582,325 to 3,583,074 ispD Position (kb) 3580 3581 3582Strain fitness (log2 ratio) -3 -2 -1 0 1at 3579.655 kb on - strand, within pcmat 3579.721 kb on - strand, within pcmat 3579.736 kb on + strand, within pcmat 3579.744 kb on - strand, within pcmat 3579.750 kb on - strand, within pcmat 3580.043 kb on + strand, within surEat 3580.141 kb on + strand, within surEat 3580.218 kb on + strand, within surEat 3580.218 kb on + strand, within surEat 3580.262 kb on + strand, within surEat 3580.411 kb on + strand, within surEat 3580.422 kb on - strand, within surEat 3580.429 kb on - strand, within surEat 3580.553 kb on + strand, within surEat 3580.652 kb on + strandat 3580.695 kb on + strandat 3580.703 kb on - strandat 3580.733 kb on - strandat 3580.831 kb on - strand, within SO3436at 3580.920 kb on - strand, within SO3436at 3581.068 kb on - strand, within SO3436at 3581.106 kb on + strand, within SO3436at 3581.168 kb on + strand, within SO3436at 3581.176 kb on - strand, within SO3436at 3581.181 kb on - strand, within SO3436at 3581.398 kb on - strand, within SO3436at 3581.510 kb on + strand, within SO3436at 3581.518 kb on - strand, within SO3436at 3581.645 kb on + strand, within SO3436at 3581.724 kb on + strandat 3581.745 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 50 mM
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3,579,655 - pcm SO3434 0.60 -1.3
3,579,721 - pcm SO3434 0.70 -0.9
3,579,736 + pcm SO3434 0.73 -1.1
3,579,744 - pcm SO3434 0.74 -0.2
3,579,750 - pcm SO3434 0.75 -0.5
3,580,043 + surE SO3435 0.18 -2.5
3,580,141 + surE SO3435 0.31 -1.7
3,580,218 + surE SO3435 0.42 -3.1
3,580,218 + surE SO3435 0.42 -3.4
3,580,262 + surE SO3435 0.48 -1.6
3,580,411 + surE SO3435 0.68 +0.3
3,580,422 - surE SO3435 0.69 -1.4
3,580,429 - surE SO3435 0.70 -1.0
3,580,553 + surE SO3435 0.87 -2.0
3,580,652 + -2.0
3,580,695 + -2.6
3,580,703 - -1.5
3,580,733 - -0.8
3,580,831 - SO3436 0.16 -1.2
3,580,920 - SO3436 0.24 -1.5
3,581,068 - SO3436 0.37 -0.1
3,581,106 + SO3436 0.41 -1.8
3,581,168 + SO3436 0.46 -1.8
3,581,176 - SO3436 0.47 -0.2
3,581,181 - SO3436 0.48 -0.6
3,581,398 - SO3436 0.67 -1.3
3,581,510 + SO3436 0.77 -1.3
3,581,518 - SO3436 0.78 -0.4
3,581,645 + SO3436 0.89 -1.9
3,581,724 + -1.6
3,581,745 + -1.7

Or see this region's nucleotide sequence