Experiment: perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcm and surE overlap by 4 nucleotides surE and SO3436 overlap by 1 nucleotides SO3436 and ispF are separated by 28 nucleotides ispF and ispD are separated by 52 nucleotides
SO3434: pcm - protein-L-isoaspartate O-methyltransferase (NCBI ptt file), at 3,579,274 to 3,579,909
pcm
SO3435: surE - stationary-phase survival protein SurE (NCBI ptt file), at 3,579,906 to 3,580,652
surE
SO3436: SO3436 - conserved hypothetical protein (NCBI ptt file), at 3,580,652 to 3,581,764
SO3436
SO3437: ispF - 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (NCBI ptt file), at 3,581,793 to 3,582,272
ispF
SO3438: ispD - 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (NCBI ptt file), at 3,582,325 to 3,583,074
ispD
Position (kb)
3580
3581
3582 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3579.655 kb on - strand, within pcm at 3579.721 kb on - strand, within pcm at 3579.736 kb on + strand, within pcm at 3579.744 kb on - strand, within pcm at 3579.750 kb on - strand, within pcm at 3580.043 kb on + strand, within surE at 3580.141 kb on + strand, within surE at 3580.218 kb on + strand, within surE at 3580.218 kb on + strand, within surE at 3580.262 kb on + strand, within surE at 3580.411 kb on + strand, within surE at 3580.422 kb on - strand, within surE at 3580.429 kb on - strand, within surE at 3580.553 kb on + strand, within surE at 3580.652 kb on + strand at 3580.695 kb on + strand at 3580.703 kb on - strand at 3580.733 kb on - strand at 3580.831 kb on - strand, within SO3436 at 3580.920 kb on - strand, within SO3436 at 3581.068 kb on - strand, within SO3436 at 3581.106 kb on + strand, within SO3436 at 3581.168 kb on + strand, within SO3436 at 3581.176 kb on - strand, within SO3436 at 3581.181 kb on - strand, within SO3436 at 3581.398 kb on - strand, within SO3436 at 3581.510 kb on + strand, within SO3436 at 3581.518 kb on - strand, within SO3436 at 3581.645 kb on + strand, within SO3436 at 3581.724 kb on + strand at 3581.745 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 50 mM remove 3,579,655 - pcm SO3434 0.60 -1.3 3,579,721 - pcm SO3434 0.70 -0.9 3,579,736 + pcm SO3434 0.73 -1.1 3,579,744 - pcm SO3434 0.74 -0.2 3,579,750 - pcm SO3434 0.75 -0.5 3,580,043 + surE SO3435 0.18 -2.5 3,580,141 + surE SO3435 0.31 -1.7 3,580,218 + surE SO3435 0.42 -3.1 3,580,218 + surE SO3435 0.42 -3.4 3,580,262 + surE SO3435 0.48 -1.6 3,580,411 + surE SO3435 0.68 +0.3 3,580,422 - surE SO3435 0.69 -1.4 3,580,429 - surE SO3435 0.70 -1.0 3,580,553 + surE SO3435 0.87 -2.0 3,580,652 + -2.0 3,580,695 + -2.6 3,580,703 - -1.5 3,580,733 - -0.8 3,580,831 - SO3436 0.16 -1.2 3,580,920 - SO3436 0.24 -1.5 3,581,068 - SO3436 0.37 -0.1 3,581,106 + SO3436 0.41 -1.8 3,581,168 + SO3436 0.46 -1.8 3,581,176 - SO3436 0.47 -0.2 3,581,181 - SO3436 0.48 -0.6 3,581,398 - SO3436 0.67 -1.3 3,581,510 + SO3436 0.77 -1.3 3,581,518 - SO3436 0.78 -0.4 3,581,645 + SO3436 0.89 -1.9 3,581,724 + -1.6 3,581,745 + -1.7
Or see this region's nucleotide sequence