Experiment: perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and ihfA are separated by 3 nucleotides ihfA and SO2088 are separated by 96 nucleotides SO2088 and hypA are separated by 85 nucleotides hypA and hypE overlap by 1 nucleotides
SO2086: pheT - phenylalanyl-tRNA synthetase, beta subunit (NCBI ptt file), at 2,181,856 to 2,184,243
pheT
SO2087: ihfA - integration host factor, alpha subunit (NCBI ptt file), at 2,184,247 to 2,184,543
ihfA
SO2088: SO2088 - lipid A biosynthesis acyltransferase, putative (NCBI ptt file), at 2,184,640 to 2,185,578
SO2088
SO2089: hypA - hydrogenase expression/formation protein HypA (NCBI ptt file), at 2,185,664 to 2,186,020
hypA
SO2090: hypE - hydrogenase expression/formation protein HypE (NCBI ptt file), at 2,186,020 to 2,187,039
hypE
Position (kb)
2184
2185
2186 Strain fitness (log2 ratio)
-2
-1
0
1 at 2184.948 kb on - strand, within SO2088 at 2184.950 kb on - strand, within SO2088 at 2185.166 kb on + strand, within SO2088 at 2185.359 kb on + strand, within SO2088 at 2185.719 kb on + strand, within hypA at 2185.769 kb on - strand, within hypA at 2185.871 kb on - strand, within hypA at 2185.900 kb on + strand, within hypA at 2186.151 kb on + strand, within hypE at 2186.267 kb on + strand, within hypE at 2186.274 kb on - strand, within hypE at 2186.454 kb on + strand, within hypE
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 50 mM remove 2,184,948 - SO2088 0.33 +0.0 2,184,950 - SO2088 0.33 -1.4 2,185,166 + SO2088 0.56 -1.8 2,185,359 + SO2088 0.77 -0.5 2,185,719 + hypA SO2089 0.15 +0.1 2,185,769 - hypA SO2089 0.29 +1.0 2,185,871 - hypA SO2089 0.58 +0.1 2,185,900 + hypA SO2089 0.66 +0.5 2,186,151 + hypE SO2090 0.13 -0.4 2,186,267 + hypE SO2090 0.24 -0.0 2,186,274 - hypE SO2090 0.25 +0.1 2,186,454 + hypE SO2090 0.43 +0.0
Or see this region's nucleotide sequence