Strain Fitness in Shewanella oneidensis MR-1 around SO2088

Experiment: perchlorate 50 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntpheT and ihfA are separated by 3 nucleotidesihfA and SO2088 are separated by 96 nucleotidesSO2088 and hypA are separated by 85 nucleotideshypA and hypE overlap by 1 nucleotides SO2086: pheT - phenylalanyl-tRNA synthetase, beta subunit (NCBI ptt file), at 2,181,856 to 2,184,243 pheT SO2087: ihfA - integration host factor, alpha subunit (NCBI ptt file), at 2,184,247 to 2,184,543 ihfA SO2088: SO2088 - lipid A biosynthesis acyltransferase, putative (NCBI ptt file), at 2,184,640 to 2,185,578 SO2088 SO2089: hypA - hydrogenase expression/formation protein HypA (NCBI ptt file), at 2,185,664 to 2,186,020 hypA SO2090: hypE - hydrogenase expression/formation protein HypE (NCBI ptt file), at 2,186,020 to 2,187,039 hypE Position (kb) 2184 2185 2186Strain fitness (log2 ratio) -2 -1 0 1at 2184.948 kb on - strand, within SO2088at 2184.950 kb on - strand, within SO2088at 2185.166 kb on + strand, within SO2088at 2185.359 kb on + strand, within SO2088at 2185.719 kb on + strand, within hypAat 2185.769 kb on - strand, within hypAat 2185.871 kb on - strand, within hypAat 2185.900 kb on + strand, within hypAat 2186.151 kb on + strand, within hypEat 2186.267 kb on + strand, within hypEat 2186.274 kb on - strand, within hypEat 2186.454 kb on + strand, within hypE

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 50 mM
remove
2,184,948 - SO2088 0.33 +0.0
2,184,950 - SO2088 0.33 -1.4
2,185,166 + SO2088 0.56 -1.8
2,185,359 + SO2088 0.77 -0.5
2,185,719 + hypA SO2089 0.15 +0.1
2,185,769 - hypA SO2089 0.29 +1.0
2,185,871 - hypA SO2089 0.58 +0.1
2,185,900 + hypA SO2089 0.66 +0.5
2,186,151 + hypE SO2090 0.13 -0.4
2,186,267 + hypE SO2090 0.24 -0.0
2,186,274 - hypE SO2090 0.25 +0.1
2,186,454 + hypE SO2090 0.43 +0.0

Or see this region's nucleotide sequence